Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models

Potential risk of nanoparticles present in food additives should be assessed. Although food-grade additive E171 (TiO2) has been banned by European Union due to the potential occurrence of nanometric TiO2, it is still present in stock products and permitted in other countries. TiO2 (nano)particles fr...

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Detalles Bibliográficos
Autores: Espada-Bernabé, Elena, Moreno Martín, Gustavo, Gómez Gómez, Beatriz, Madrid Albarrán, María Yolanda
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/92083
Acceso en línea:https://hdl.handle.net/20.500.14352/92083
Access Level:acceso abierto
Palabra clave:543
Chocolate candies
TiO2 (nano)particles
spICP-MS
In vitro gastrointestinal digestion
Caco-2 cells
Ciencias
2301 Química Analítica
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spelling Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro modelsEspada-Bernabé, ElenaMoreno Martín, GustavoGómez Gómez, BeatrizMadrid Albarrán, María Yolanda543Chocolate candiesTiO2 (nano)particlesspICP-MSIn vitro gastrointestinal digestionCaco-2 cellsCiencias2301 Química AnalíticaPotential risk of nanoparticles present in food additives should be assessed. Although food-grade additive E171 (TiO2) has been banned by European Union due to the potential occurrence of nanometric TiO2, it is still present in stock products and permitted in other countries. TiO2 (nano)particles from the outer coating of colored chocolate candies were determined by TEM (Transmission Electron Microscopy), ICP-MS (Inductively Coupled Plasma Mass Spectrometry) and spICP-MS (Single-Particle-ICP-MS). Total titanium content was in the range of 1219 ± 83 μg/g, except for brown and white candies. Percentage of TiO2 particles bellow 100 nm was under 25% regardless the color, with most frequent size between 120 and 160 nm. In vitro gastrointestinal assays reveal differences in bioaccessibility percentages between whole candy (14%) or aqueous extracts of the coating of candy (37%). More than 90% of bioaccessible titanium was found in particulate form. Caco-2 cells viability decreased around 65% after 24 h exposed to intestinal fraction.ElsevierUniversidad Complutense de Madrid20232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/92083reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/920832026-06-02T12:44:21Z
dc.title.none.fl_str_mv Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
title Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
spellingShingle Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
Espada-Bernabé, Elena
543
Chocolate candies
TiO2 (nano)particles
spICP-MS
In vitro gastrointestinal digestion
Caco-2 cells
Ciencias
2301 Química Analítica
title_short Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
title_full Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
title_fullStr Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
title_full_unstemmed Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
title_sort Assesing the behaviour of particulate/nanoparticulate form of E171 (TiO2) food additive in colored chocolate candies before and after in vitro oral ingestion by spICP-MS, TEM and cellular in vitro models
dc.creator.none.fl_str_mv Espada-Bernabé, Elena
Moreno Martín, Gustavo
Gómez Gómez, Beatriz
Madrid Albarrán, María Yolanda
author Espada-Bernabé, Elena
author_facet Espada-Bernabé, Elena
Moreno Martín, Gustavo
Gómez Gómez, Beatriz
Madrid Albarrán, María Yolanda
author_role author
author2 Moreno Martín, Gustavo
Gómez Gómez, Beatriz
Madrid Albarrán, María Yolanda
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Chocolate candies
TiO2 (nano)particles
spICP-MS
In vitro gastrointestinal digestion
Caco-2 cells
Ciencias
2301 Química Analítica
topic 543
Chocolate candies
TiO2 (nano)particles
spICP-MS
In vitro gastrointestinal digestion
Caco-2 cells
Ciencias
2301 Química Analítica
description Potential risk of nanoparticles present in food additives should be assessed. Although food-grade additive E171 (TiO2) has been banned by European Union due to the potential occurrence of nanometric TiO2, it is still present in stock products and permitted in other countries. TiO2 (nano)particles from the outer coating of colored chocolate candies were determined by TEM (Transmission Electron Microscopy), ICP-MS (Inductively Coupled Plasma Mass Spectrometry) and spICP-MS (Single-Particle-ICP-MS). Total titanium content was in the range of 1219 ± 83 μg/g, except for brown and white candies. Percentage of TiO2 particles bellow 100 nm was under 25% regardless the color, with most frequent size between 120 and 160 nm. In vitro gastrointestinal assays reveal differences in bioaccessibility percentages between whole candy (14%) or aqueous extracts of the coating of candy (37%). More than 90% of bioaccessible titanium was found in particulate form. Caco-2 cells viability decreased around 65% after 24 h exposed to intestinal fraction.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/92083
url https://hdl.handle.net/20.500.14352/92083
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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