Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts

This dataset characterizes various N-Acetylglucosamine oxidase (NagOX) constructs fused with different affinity and solubility-enhancing tags (6xHis, SUMO, CBM3, and CBM9) to evaluate their hydrogen peroxide (H2O2) production. 6xHis-SUMO-NagOX and 6xHis-SUMO-CBM3-NagOX were selected for comparative...

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Detalhes bibliográficos
Autores: Fredes, Yerko, Dimitri Gómez, Ivan, Guerra-Mas, Gerard, Casablancas, Antoni, Santema, Lars, Fraaije, Marco, Romero, Oscar, Guillén, Marina
Formato: conjunto de datos
Fecha de publicación:2026
País:España
Recursos:Consorci de Serveis Universitaris de Catalunya (CSUC)
Repositorio:CORA.Repositori de Dades de Recerca
OAI Identifier:oai:dnet:cora.rdr____::e25054879ca0a9b9717a9314777b65e7
Acesso em linha:https://doi.org/10.34810/DATA2997
Access Level:acceso abierto
Palavra-chave:Chemistry
Earth and Environmental Sciences
Engineering
Affinity fusion tag
Carbohydrate binding module (CBM)
In situ Hydrogen peroxide (H2O2) generation
Enzyme purification
Cellulose
Carbohydrate oxidases
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spelling Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating BiocatalystsFredes, YerkoDimitri Gómez, IvanGuerra-Mas, GerardCasablancas, AntoniSantema, LarsFraaije, MarcoRomero, OscarGuillén, MarinaChemistryEarth and Environmental SciencesEngineeringAffinity fusion tagCarbohydrate binding module (CBM)In situ Hydrogen peroxide (H2O2) generationEnzyme purificationCelluloseCarbohydrate oxidasesThis dataset characterizes various N-Acetylglucosamine oxidase (NagOX) constructs fused with different affinity and solubility-enhancing tags (6xHis, SUMO, CBM3, and CBM9) to evaluate their hydrogen peroxide (H2O2) production. 6xHis-SUMO-NagOX and 6xHis-SUMO-CBM3-NagOX were selected for comparative immobilization and purification studies, using the absence of catalase activity as a key selection criterion. 6xHis-SUMO-CBM9-NagOX was excluded from further testing due to observed proteolysis, which compromised the enzyme's structural integrity. Ultimately, the CBM3/Cellulose system (Perloza MT100 medium) yielded a higher purification factor and superior immobilization results, as it successfully eliminated catalase from the final biocatalyst.CORA.Repositori de Dades de RecercaFredes Tapia, Yerko2026info:eu-repo/semantics/datasethttps://doi.org/10.34810/DATA2997reponame:CORA.Repositori de Dades de Recercainstname:Consorci de Serveis Universitaris de Catalunya (CSUC)Inglésinfo:eu-repo/semantics/openAccessCC0 1.0oai:dnet:cora.rdr____::e25054879ca0a9b9717a9314777b65e72026-06-17T12:20:17Z
dc.title.none.fl_str_mv Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
title Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
spellingShingle Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
Fredes, Yerko
Chemistry
Earth and Environmental Sciences
Engineering
Affinity fusion tag
Carbohydrate binding module (CBM)
In situ Hydrogen peroxide (H2O2) generation
Enzyme purification
Cellulose
Carbohydrate oxidases
title_short Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
title_full Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
title_fullStr Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
title_full_unstemmed Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
title_sort Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
dc.creator.none.fl_str_mv Fredes, Yerko
Dimitri Gómez, Ivan
Guerra-Mas, Gerard
Casablancas, Antoni
Santema, Lars
Fraaije, Marco
Romero, Oscar
Guillén, Marina
author Fredes, Yerko
author_facet Fredes, Yerko
Dimitri Gómez, Ivan
Guerra-Mas, Gerard
Casablancas, Antoni
Santema, Lars
Fraaije, Marco
Romero, Oscar
Guillén, Marina
author_role author
author2 Dimitri Gómez, Ivan
Guerra-Mas, Gerard
Casablancas, Antoni
Santema, Lars
Fraaije, Marco
Romero, Oscar
Guillén, Marina
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fredes Tapia, Yerko
dc.subject.none.fl_str_mv Chemistry
Earth and Environmental Sciences
Engineering
Affinity fusion tag
Carbohydrate binding module (CBM)
In situ Hydrogen peroxide (H2O2) generation
Enzyme purification
Cellulose
Carbohydrate oxidases
topic Chemistry
Earth and Environmental Sciences
Engineering
Affinity fusion tag
Carbohydrate binding module (CBM)
In situ Hydrogen peroxide (H2O2) generation
Enzyme purification
Cellulose
Carbohydrate oxidases
description This dataset characterizes various N-Acetylglucosamine oxidase (NagOX) constructs fused with different affinity and solubility-enhancing tags (6xHis, SUMO, CBM3, and CBM9) to evaluate their hydrogen peroxide (H2O2) production. 6xHis-SUMO-NagOX and 6xHis-SUMO-CBM3-NagOX were selected for comparative immobilization and purification studies, using the absence of catalase activity as a key selection criterion. 6xHis-SUMO-CBM9-NagOX was excluded from further testing due to observed proteolysis, which compromised the enzyme's structural integrity. Ultimately, the CBM3/Cellulose system (Perloza MT100 medium) yielded a higher purification factor and superior immobilization results, as it successfully eliminated catalase from the final biocatalyst.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://doi.org/10.34810/DATA2997
url https://doi.org/10.34810/DATA2997
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
CC0 1.0
eu_rights_str_mv openAccess
rights_invalid_str_mv CC0 1.0
dc.publisher.none.fl_str_mv CORA.Repositori de Dades de Recerca
publisher.none.fl_str_mv CORA.Repositori de Dades de Recerca
dc.source.none.fl_str_mv reponame:CORA.Repositori de Dades de Recerca
instname:Consorci de Serveis Universitaris de Catalunya (CSUC)
instname_str Consorci de Serveis Universitaris de Catalunya (CSUC)
reponame_str CORA.Repositori de Dades de Recerca
collection CORA.Repositori de Dades de Recerca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418403079389184
score 15,81155

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