Replication Data for: Affinity Tag Selection as a Process Tool to Eliminate Catalase Contamination in H₂O₂-Generating Biocatalysts
This dataset characterizes various N-Acetylglucosamine oxidase (NagOX) constructs fused with different affinity and solubility-enhancing tags (6xHis, SUMO, CBM3, and CBM9) to evaluate their hydrogen peroxide (H2O2) production. 6xHis-SUMO-NagOX and 6xHis-SUMO-CBM3-NagOX were selected for comparative...
| Autores: | , , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Consorci de Serveis Universitaris de Catalunya (CSUC) |
| Repositorio: | CORA.Repositori de Dades de Recerca |
| OAI Identifier: | oai:dnet:cora.rdr____::e25054879ca0a9b9717a9314777b65e7 |
| Acceso en línea: | https://doi.org/10.34810/DATA2997 |
| Access Level: | acceso abierto |
| Palabra clave: | Chemistry Earth and Environmental Sciences Engineering Affinity fusion tag Carbohydrate binding module (CBM) In situ Hydrogen peroxide (H2O2) generation Enzyme purification Cellulose Carbohydrate oxidases |
| Sumario: | This dataset characterizes various N-Acetylglucosamine oxidase (NagOX) constructs fused with different affinity and solubility-enhancing tags (6xHis, SUMO, CBM3, and CBM9) to evaluate their hydrogen peroxide (H2O2) production. 6xHis-SUMO-NagOX and 6xHis-SUMO-CBM3-NagOX were selected for comparative immobilization and purification studies, using the absence of catalase activity as a key selection criterion. 6xHis-SUMO-CBM9-NagOX was excluded from further testing due to observed proteolysis, which compromised the enzyme's structural integrity. Ultimately, the CBM3/Cellulose system (Perloza MT100 medium) yielded a higher purification factor and superior immobilization results, as it successfully eliminated catalase from the final biocatalyst. |
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