The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae

The tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of la...

Descripción completa

Detalles Bibliográficos
Autores: Cuevas Bermúdez, Abel, Martínez Fernández, Verónica, Garrido Godino, Ana Isabel, Jordán Pla, Antonio, Peñate Salas, Xenia, Martín Expósito, Manuel, Gutiérrez Pozo, Gabriel, Govind, Chhabi K., Chávez de Diego, Sebastián, Pelechano, Vicent, Navarro, Francisco
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/179283
Acceso en línea:https://hdl.handle.net/11441/179283
https://doi.org/10.1016/j.bbagrm.2023.194995
Access Level:acceso abierto
Palabra clave:Prefoldin-like
Transcription
RNA polymerases
Alternative polyadenylation
29 mRNA stability
Chromatin
Saccharomyces cerevisiae
id ES_be8f7a8b46dff4b0a191df75db137770
oai_identifier_str oai:idus.us.es:11441/179283
network_acronym_str ES
network_name_str España
repository_id_str
spelling The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiaeCuevas Bermúdez, AbelMartínez Fernández, VerónicaGarrido Godino, Ana IsabelJordán Pla, AntonioPeñate Salas, XeniaMartín Expósito, ManuelGutiérrez Pozo, GabrielGovind, Chhabi K.Chávez de Diego, SebastiánPelechano, VicentNavarro, FranciscoPrefoldin-likeTranscriptionRNA polymerasesAlternative polyadenylation29 mRNA stabilityChromatinSaccharomyces cerevisiaeThe tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of lack of Bud27 on the chromatin association of RSC and RNA pol II, and on nucleosome positioning. Our results demonstrate that RSC binds chromatin in gene bodies and lack of Bud27 alters this association, mainly around polyA sites. This alteration impacts chromatin organization and leads to the accumulation of RNA pol II molecules around polyA sites, likely due to pausing or arrest. Our data suggest that RSC is necessary to maintain chromatin organization around those sites, and any alteration of this organization results in the widespread use of alternative polyA sites. Finally, we also find a similar molecular phenotype that occurs upon TOR inhibition with rapamycin, which suggests that alternative polyadenylation observed upon TOR inhibition is likely Bud27-dependent.ElsevierGenéticaMinisterio de Ciencia e Innovación (MICIN). EspañaAgencia Estatal de Investigación. EspañaEuropean Union (UE)Junta de AndalucíaSwedish Research Council2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/179283https://doi.org/10.1016/j.bbagrm.2023.194995reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBiochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1867, 194995.PID2020-112853GB-C33PID2020-112853GB-C32RED2018-102467-TFEDER-UJA 1260360P20-00792VR 2019-02335VR 2020-01480VR 2021-06112https://doi.org/10.1016/j.bbagrm.2023.194995info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1792832026-06-17T12:51:07Z
dc.title.none.fl_str_mv The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
title The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
spellingShingle The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
Cuevas Bermúdez, Abel
Prefoldin-like
Transcription
RNA polymerases
Alternative polyadenylation
29 mRNA stability
Chromatin
Saccharomyces cerevisiae
title_short The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
title_full The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
title_fullStr The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
title_full_unstemmed The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
title_sort The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae
dc.creator.none.fl_str_mv Cuevas Bermúdez, Abel
Martínez Fernández, Verónica
Garrido Godino, Ana Isabel
Jordán Pla, Antonio
Peñate Salas, Xenia
Martín Expósito, Manuel
Gutiérrez Pozo, Gabriel
Govind, Chhabi K.
Chávez de Diego, Sebastián
Pelechano, Vicent
Navarro, Francisco
author Cuevas Bermúdez, Abel
author_facet Cuevas Bermúdez, Abel
Martínez Fernández, Verónica
Garrido Godino, Ana Isabel
Jordán Pla, Antonio
Peñate Salas, Xenia
Martín Expósito, Manuel
Gutiérrez Pozo, Gabriel
Govind, Chhabi K.
Chávez de Diego, Sebastián
Pelechano, Vicent
Navarro, Francisco
author_role author
author2 Martínez Fernández, Verónica
Garrido Godino, Ana Isabel
Jordán Pla, Antonio
Peñate Salas, Xenia
Martín Expósito, Manuel
Gutiérrez Pozo, Gabriel
Govind, Chhabi K.
Chávez de Diego, Sebastián
Pelechano, Vicent
Navarro, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Genética
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
European Union (UE)
Junta de Andalucía
Swedish Research Council
dc.subject.none.fl_str_mv Prefoldin-like
Transcription
RNA polymerases
Alternative polyadenylation
29 mRNA stability
Chromatin
Saccharomyces cerevisiae
topic Prefoldin-like
Transcription
RNA polymerases
Alternative polyadenylation
29 mRNA stability
Chromatin
Saccharomyces cerevisiae
description The tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of lack of Bud27 on the chromatin association of RSC and RNA pol II, and on nucleosome positioning. Our results demonstrate that RSC binds chromatin in gene bodies and lack of Bud27 alters this association, mainly around polyA sites. This alteration impacts chromatin organization and leads to the accumulation of RNA pol II molecules around polyA sites, likely due to pausing or arrest. Our data suggest that RSC is necessary to maintain chromatin organization around those sites, and any alteration of this organization results in the widespread use of alternative polyA sites. Finally, we also find a similar molecular phenotype that occurs upon TOR inhibition with rapamycin, which suggests that alternative polyadenylation observed upon TOR inhibition is likely Bud27-dependent.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/179283
https://doi.org/10.1016/j.bbagrm.2023.194995
url https://hdl.handle.net/11441/179283
https://doi.org/10.1016/j.bbagrm.2023.194995
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 1867, 194995.
PID2020-112853GB-C33
PID2020-112853GB-C32
RED2018-102467-T
FEDER-UJA 1260360
P20-00792
VR 2019-02335
VR 2020-01480
VR 2021-06112
https://doi.org/10.1016/j.bbagrm.2023.194995
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418295569940480
score 15,812429

Similares en LA Referencia