The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae

The tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of la...

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Detalles Bibliográficos
Autores: Cuevas Bermúdez, Abel, Martínez Fernández, Verónica, Garrido Godino, Ana Isabel, Jordán Pla, Antonio, Peñate Salas, Xenia, Martín Expósito, Manuel, Gutiérrez Pozo, Gabriel, Govind, Chhabi K., Chávez de Diego, Sebastián, Pelechano, Vicent, Navarro, Francisco
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/179283
Acceso en línea:https://hdl.handle.net/11441/179283
https://doi.org/10.1016/j.bbagrm.2023.194995
Access Level:acceso abierto
Palabra clave:Prefoldin-like
Transcription
RNA polymerases
Alternative polyadenylation
29 mRNA stability
Chromatin
Saccharomyces cerevisiae
Descripción
Sumario:The tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of lack of Bud27 on the chromatin association of RSC and RNA pol II, and on nucleosome positioning. Our results demonstrate that RSC binds chromatin in gene bodies and lack of Bud27 alters this association, mainly around polyA sites. This alteration impacts chromatin organization and leads to the accumulation of RNA pol II molecules around polyA sites, likely due to pausing or arrest. Our data suggest that RSC is necessary to maintain chromatin organization around those sites, and any alteration of this organization results in the widespread use of alternative polyA sites. Finally, we also find a similar molecular phenotype that occurs upon TOR inhibition with rapamycin, which suggests that alternative polyadenylation observed upon TOR inhibition is likely Bud27-dependent.

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