Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase

In this work, a very useful and accurate procedure, based on the spectrophotometric method published by the American Public Health Association in the Standards Methods for the Examination of Water and Wastewater, was developed to determine cell density of Isochrysis galbana performing a single direc...

ver descrição completa

Detalhes bibliográficos
Autores: Pavía Gómez, Mireia, García Romeral, Julia, Chirivella Martorell, Jerónimo, Serrano Aroca, Ángel
Formato: artículo
Fecha de publicación:2016
País:España
Recursos:Universidad Católica de Valencia San Vicente Mártir
Repositorio:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
Idioma:inglés
OAI Identifier:oai:riucv.ucv.es:20.500.12466/238
Acesso em linha:http://hdl.handle.net/20.500.12466/238
Access Level:acceso abierto
Palavra-chave:Microalgae
Water analysis
Microalgas
Análisis del agua
3390.01 Biotecnología de Microalgas
2508.11 Calidad de las Aguas
id ES_be2ca63dbfbeecd6d89132b50ac47900
oai_identifier_str oai:riucv.ucv.es:20.500.12466/238
network_acronym_str ES
network_name_str España
repository_id_str
spelling Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phasePavía Gómez, MireiaGarcía Romeral, JuliaChirivella Martorell, JerónimoSerrano Aroca, ÁngelMicroalgaeWater analysisMicroalgasAnálisis del agua3390.01 Biotecnología de Microalgas2508.11 Calidad de las AguasIn this work, a very useful and accurate procedure, based on the spectrophotometric method published by the American Public Health Association in the Standards Methods for the Examination of Water and Wastewater, was developed to determine cell density of Isochrysis galbana performing a single direct absorbance measurement in exponential phase of growth, which is the desirable operating mode for any microalgae production plant. Thus, Isochrysis galbana was cultured in serial batch cultures from a larger scale fed-batch culture. The growth performance of this species of microalgae under laboratory conditions was analysed by spectrophotometry at different wavelengths and cell counting in a haemocytometer (Neubauer chamber) showing that doubling times and cell death increased with increasing initial cell density. Besides, it was demonstrated that the absorbance of these cultures followed a linear trend as a function of time and cell density during the exponential phase of growth, results in which the developed direct method is based on.En este trabajo, se ha determinado un procedimiento muy útil y preciso basado en el método espectrofotométrico descrito por la Asociación Americana Pública de Salud en los Métodos Estándar para el Análisis de Aguas y Aguas Residuales, para calcular la densidad celular de cultivos de Isochrysis galbana realizando una única medida de absorbancia en fase exponencial de crecimiento que es el modo de trabajo deseable de cualquier planta de producción de microalgas. De este modo, se realizó una serie de cultivos de Isochrysis galbana en discontinuo a partir de otro cultivo a mayor escala con alimentación intermitente. Se ha analizado el nivel de crecimiento de esta especie de microalga por espectrofotometría a diferentes longitudes de onda y recuento de células en un hemocitómetro (cámara de Neubauer) mostrando que el tiemplo de duplicación y la muerte celular aumentó con el aumento de la densidad celular inicial. Además, se ha demostrado que la absorbancia de estos cultivos sigue una tendencia lineal en función del tiempo y densidad celular durante la fase de crecimiento exponencial, resultados en los cuales se basa el método directo desarrollado.20192019-10-2320162016-03-0120162016-03-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12466/238reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártirinstname:Universidad Católica de Valencia San Vicente MártirInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riucv.ucv.es:20.500.12466/2382026-06-19T08:32:07Z
dc.title.none.fl_str_mv Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
title Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
spellingShingle Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
Pavía Gómez, Mireia
Microalgae
Water analysis
Microalgas
Análisis del agua
3390.01 Biotecnología de Microalgas
2508.11 Calidad de las Aguas
title_short Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
title_full Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
title_fullStr Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
title_full_unstemmed Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
title_sort Direct spectrophotometric method to determine cell density of Isochrysis galbana in serial batch cultures from a larger scale fed-batch culture in exponential phase
dc.creator.none.fl_str_mv Pavía Gómez, Mireia
García Romeral, Julia
Chirivella Martorell, Jerónimo
Serrano Aroca, Ángel
author Pavía Gómez, Mireia
author_facet Pavía Gómez, Mireia
García Romeral, Julia
Chirivella Martorell, Jerónimo
Serrano Aroca, Ángel
author_role author
author2 García Romeral, Julia
Chirivella Martorell, Jerónimo
Serrano Aroca, Ángel
author2_role author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Microalgae
Water analysis
Microalgas
Análisis del agua
3390.01 Biotecnología de Microalgas
2508.11 Calidad de las Aguas
topic Microalgae
Water analysis
Microalgas
Análisis del agua
3390.01 Biotecnología de Microalgas
2508.11 Calidad de las Aguas
description In this work, a very useful and accurate procedure, based on the spectrophotometric method published by the American Public Health Association in the Standards Methods for the Examination of Water and Wastewater, was developed to determine cell density of Isochrysis galbana performing a single direct absorbance measurement in exponential phase of growth, which is the desirable operating mode for any microalgae production plant. Thus, Isochrysis galbana was cultured in serial batch cultures from a larger scale fed-batch culture. The growth performance of this species of microalgae under laboratory conditions was analysed by spectrophotometry at different wavelengths and cell counting in a haemocytometer (Neubauer chamber) showing that doubling times and cell death increased with increasing initial cell density. Besides, it was demonstrated that the absorbance of these cultures followed a linear trend as a function of time and cell density during the exponential phase of growth, results in which the developed direct method is based on.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-03-01
2016
2016-03-01
2019
2019-10-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12466/238
url http://hdl.handle.net/20.500.12466/238
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
instname:Universidad Católica de Valencia San Vicente Mártir
instname_str Universidad Católica de Valencia San Vicente Mártir
reponame_str RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
collection RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418263172087808
score 15,300719

Registos relacionados