Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators

Trametes trogii BAFC 463 culture fluids (containing 110Uml<sup>-1</sup>laccase; 0.94Uml<sup>-1</sup> manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of...

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Autores: Grassi, Emanuel, Scodeller, Pablo, Filiel, Nestor, Carballo, Romina, Levin, Laura
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/412959
Acceso en línea:http://hdl.handle.net/10261/412959
https://api.elsevier.com/content/abstract/scopus_id/79955828431
Access Level:acceso abierto
Palabra clave:White-rot fungi
Dye decolorization
Laccase
Manganese peroxidase
Redox mediators
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
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spelling Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediatorsGrassi, EmanuelScodeller, PabloFiliel, NestorCarballo, RominaLevin, LauraWhite-rot fungiDye decolorizationLaccaseManganese peroxidaseRedox mediatorshttp://metadata.un.org/sdg/3http://metadata.un.org/sdg/9Ensure healthy lives and promote well-being for all at all agesBuild resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationTrametes trogii BAFC 463 culture fluids (containing 110Uml<sup>-1</sup>laccase; 0.94Uml<sup>-1</sup> manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70°C and pH 7.0, even in the presence of high concentrations of heavy metals (100mM Cu<sup>+2</sup>, Pb<sup>+2</sup> or Cd<sup>+2</sup>) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents. © 2011 Elsevier Ltd.The authors are grateful to Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina) and University of Buenos Aires for financial support.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262011info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/412959https://api.elsevier.com/content/abstract/scopus_id/79955828431reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInternational Biodeterioration and Biodegradationhttps://doi.org/10.1016/j.ibiod.2011.03.007Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4129592026-05-22T06:33:51Z
dc.title.none.fl_str_mv Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
spellingShingle Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
Grassi, Emanuel
White-rot fungi
Dye decolorization
Laccase
Manganese peroxidase
Redox mediators
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
title_short Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_full Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_fullStr Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_full_unstemmed Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
title_sort Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
dc.creator.none.fl_str_mv Grassi, Emanuel
Scodeller, Pablo
Filiel, Nestor
Carballo, Romina
Levin, Laura
author Grassi, Emanuel
author_facet Grassi, Emanuel
Scodeller, Pablo
Filiel, Nestor
Carballo, Romina
Levin, Laura
author_role author
author2 Scodeller, Pablo
Filiel, Nestor
Carballo, Romina
Levin, Laura
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv White-rot fungi
Dye decolorization
Laccase
Manganese peroxidase
Redox mediators
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
topic White-rot fungi
Dye decolorization
Laccase
Manganese peroxidase
Redox mediators
http://metadata.un.org/sdg/3
http://metadata.un.org/sdg/9
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
description Trametes trogii BAFC 463 culture fluids (containing 110Uml<sup>-1</sup>laccase; 0.94Uml<sup>-1</sup> manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70°C and pH 7.0, even in the presence of high concentrations of heavy metals (100mM Cu<sup>+2</sup>, Pb<sup>+2</sup> or Cd<sup>+2</sup>) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents. © 2011 Elsevier Ltd.
publishDate 2011
dc.date.none.fl_str_mv 2011
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/412959
https://api.elsevier.com/content/abstract/scopus_id/79955828431
url http://hdl.handle.net/10261/412959
https://api.elsevier.com/content/abstract/scopus_id/79955828431
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International Biodeterioration and Biodegradation
https://doi.org/10.1016/j.ibiod.2011.03.007
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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