Potential of Trametes trogii culture fluids and its purified laccase for the decolorization of different types of recalcitrant dyes without the addition of redox mediators
Trametes trogii BAFC 463 culture fluids (containing 110Uml<sup>-1</sup>laccase; 0.94Uml<sup>-1</sup> manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/412959 |
| Acceso en línea: | http://hdl.handle.net/10261/412959 https://api.elsevier.com/content/abstract/scopus_id/79955828431 |
| Access Level: | acceso abierto |
| Palabra clave: | White-rot fungi Dye decolorization Laccase Manganese peroxidase Redox mediators http://metadata.un.org/sdg/3 http://metadata.un.org/sdg/9 Ensure healthy lives and promote well-being for all at all ages Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation |
| Sumario: | Trametes trogii BAFC 463 culture fluids (containing 110Uml<sup>-1</sup>laccase; 0.94Uml<sup>-1</sup> manganese peroxidase), as well as its purified laccase were capable of decolorizing azoic, indigoid, triphenylmethane, anthraquinonic and heterocyclic dyes, in the absence of redox mediators. Six dyes: RBBR, Indigo Carmine, Xylidine, Malachite Green, Gentian Violet and Bromophenol Blue were almost completely degraded (more than 85% decolorization after 1 d) by either laccase or T. trogii itself in culture, proving the role of the enzyme in dye decolorization. The purified laccase also decolorized 65% of Fast Blue RR and 30% of Azure B and Methylene Blue after 24h. The use of redox mediators significantly increased the decolorization rates (90% decolorization of Azure B after 1h). 1-hydroxybenzotriazole resulted the best redox mediator, but the natural mediator p-hydroxybenzoic acid also demonstrated its efficiency for dye decolorization. Due to their ability to decolorize recalcitrant dyes without addition of redox mediators, high laccase activities, high thermostability and efficient decolorization at 70°C and pH 7.0, even in the presence of high concentrations of heavy metals (100mM Cu<sup>+2</sup>, Pb<sup>+2</sup> or Cd<sup>+2</sup>) or in a synthetic dyebath, T. trogii culture fluids could be effectively used to decolorize synthetic dyes from effluents. © 2011 Elsevier Ltd. |
|---|