A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes
A novel microfluidic paper-based analytical device with dual colorimetric and electrochemical detection (dual μPAD) was developed for the assessment of transferrin saturation (TSAT) in samples from ischemic stroke patients. TSAT was calculated from the ratio between transferrin-bound iron, which was...
| Autores: | , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:311554 |
| Acceso en línea: | https://ddd.uab.cat/record/311554 https://dx.doi.org/urn:doi:10.1039/d4lc00398e |
| Access Level: | acceso abierto |
| Palabra clave: | Colorimetric detection ELectrochemical detection Electrochemicals Ischemic strokes Paper-based analytical devices Point-of-care testing Preloading Reagent-free Stroke patients Total iron binding capacities Colorimetry Electrochemical Techniques Equipment Design Humans Iron Ischemic Stroke Lab-On-A-Chip Devices Microfluidic Analytical Techniques Paper Point-of-Care Testing Transferrin |
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A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokesDortez Herranz, Silvia|||0000-0002-1999-5605Pacheco Jerez, Marta|||0000-0001-9546-9108Gasull Dalmau, Teresa|||0000-0002-9321-1741Crevillen, Agustín G.|||0000-0002-4470-6502Escarpa, Alberto|||0000-0002-7302-0948Colorimetric detectionELectrochemical detectionElectrochemicalsIschemic strokesPaper-based analytical devicesPoint-of-care testingPreloadingReagent-freeStroke patientsTotal iron binding capacitiesColorimetryElectrochemical TechniquesEquipment DesignHumansIronIschemic StrokeLab-On-A-Chip DevicesMicrofluidic Analytical TechniquesPaperPoint-of-Care TestingTransferrinA novel microfluidic paper-based analytical device with dual colorimetric and electrochemical detection (dual μPAD) was developed for the assessment of transferrin saturation (TSAT) in samples from ischemic stroke patients. TSAT was calculated from the ratio between transferrin-bound iron, which was colorimetrically measured, and the total iron-binding capacity, which was electrochemically measured. To this end, a μPAD was smartly designed, which integrated both colorimetric and electrochemical detection reservoirs, communicating via a microchannel acting as a chemical reactor, and with preloading/storing capabilities (reagent-free device). This approach allowed the dual and simultaneous determination of both parameters, providing an improvement in the reliability of the results due to an independent signal principle and processing. The μPADs were validated by analyzing a certified reference material, showing excellent accuracy (E ≤ 5%) and precision (RSD ≤ 2%). Then they were applied to the analysis of diagnosed serum samples from ischemic stroke patients. The results were compared to those provided by a free-interference method (urea-PAGE). Impressively, both methods exhibited a good correlation (r = 0.96, p < 0.05) and no significant differences were found between them (slope 1.0 ± 0.1 and the intercept 1 ± 4, p < 0.05), demonstrating the excellent accuracy of our approach during the analysis of complex samples from ischemic stroke patients, using just 90 μL of clinical samples and taking less than 90 min in comparison with the 18 hours required by the urea-PAGE approach. The developed fully integrated colorimetric-electrochemical μPAD is a promising ready to use reagent-free device for the point-of-care testing of TSAT, which can be used to assist physicians in the fast diagnosis and prognosis of ischemic strokes, where the decision-time is crucial for the patient's survival. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/311554https://dx.doi.org/urn:doi:10.1039/d4lc00398ereponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 CTQ2017-86441-C2-1-RAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-118154GB-I00Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00925open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3115542026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| title |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| spellingShingle |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes Dortez Herranz, Silvia|||0000-0002-1999-5605 Colorimetric detection ELectrochemical detection Electrochemicals Ischemic strokes Paper-based analytical devices Point-of-care testing Preloading Reagent-free Stroke patients Total iron binding capacities Colorimetry Electrochemical Techniques Equipment Design Humans Iron Ischemic Stroke Lab-On-A-Chip Devices Microfluidic Analytical Techniques Paper Point-of-Care Testing Transferrin |
| title_short |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| title_full |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| title_fullStr |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| title_full_unstemmed |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| title_sort |
A dual colorimetric-electrochemical microfluidic paper-based analytical device for point-of-care testing of ischemic strokes |
| dc.creator.none.fl_str_mv |
Dortez Herranz, Silvia|||0000-0002-1999-5605 Pacheco Jerez, Marta|||0000-0001-9546-9108 Gasull Dalmau, Teresa|||0000-0002-9321-1741 Crevillen, Agustín G.|||0000-0002-4470-6502 Escarpa, Alberto|||0000-0002-7302-0948 |
| author |
Dortez Herranz, Silvia|||0000-0002-1999-5605 |
| author_facet |
Dortez Herranz, Silvia|||0000-0002-1999-5605 Pacheco Jerez, Marta|||0000-0001-9546-9108 Gasull Dalmau, Teresa|||0000-0002-9321-1741 Crevillen, Agustín G.|||0000-0002-4470-6502 Escarpa, Alberto|||0000-0002-7302-0948 |
| author_role |
author |
| author2 |
Pacheco Jerez, Marta|||0000-0001-9546-9108 Gasull Dalmau, Teresa|||0000-0002-9321-1741 Crevillen, Agustín G.|||0000-0002-4470-6502 Escarpa, Alberto|||0000-0002-7302-0948 |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Colorimetric detection ELectrochemical detection Electrochemicals Ischemic strokes Paper-based analytical devices Point-of-care testing Preloading Reagent-free Stroke patients Total iron binding capacities Colorimetry Electrochemical Techniques Equipment Design Humans Iron Ischemic Stroke Lab-On-A-Chip Devices Microfluidic Analytical Techniques Paper Point-of-Care Testing Transferrin |
| topic |
Colorimetric detection ELectrochemical detection Electrochemicals Ischemic strokes Paper-based analytical devices Point-of-care testing Preloading Reagent-free Stroke patients Total iron binding capacities Colorimetry Electrochemical Techniques Equipment Design Humans Iron Ischemic Stroke Lab-On-A-Chip Devices Microfluidic Analytical Techniques Paper Point-of-Care Testing Transferrin |
| description |
A novel microfluidic paper-based analytical device with dual colorimetric and electrochemical detection (dual μPAD) was developed for the assessment of transferrin saturation (TSAT) in samples from ischemic stroke patients. TSAT was calculated from the ratio between transferrin-bound iron, which was colorimetrically measured, and the total iron-binding capacity, which was electrochemically measured. To this end, a μPAD was smartly designed, which integrated both colorimetric and electrochemical detection reservoirs, communicating via a microchannel acting as a chemical reactor, and with preloading/storing capabilities (reagent-free device). This approach allowed the dual and simultaneous determination of both parameters, providing an improvement in the reliability of the results due to an independent signal principle and processing. The μPADs were validated by analyzing a certified reference material, showing excellent accuracy (E ≤ 5%) and precision (RSD ≤ 2%). Then they were applied to the analysis of diagnosed serum samples from ischemic stroke patients. The results were compared to those provided by a free-interference method (urea-PAGE). Impressively, both methods exhibited a good correlation (r = 0.96, p < 0.05) and no significant differences were found between them (slope 1.0 ± 0.1 and the intercept 1 ± 4, p < 0.05), demonstrating the excellent accuracy of our approach during the analysis of complex samples from ischemic stroke patients, using just 90 μL of clinical samples and taking less than 90 min in comparison with the 18 hours required by the urea-PAGE approach. The developed fully integrated colorimetric-electrochemical μPAD is a promising ready to use reagent-free device for the point-of-care testing of TSAT, which can be used to assist physicians in the fast diagnosis and prognosis of ischemic strokes, where the decision-time is crucial for the patient's survival. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2 2024-01-01 2024 2024-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/311554 https://dx.doi.org/urn:doi:10.1039/d4lc00398e |
| url |
https://ddd.uab.cat/record/311554 https://dx.doi.org/urn:doi:10.1039/d4lc00398e |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 CTQ2017-86441-C2-1-R Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-118154GB-I00 Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00925 |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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