Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for f...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de documento: | artigo |
| Data de publicação: | 2021 |
| País: | España |
| Recursos: | Universitat Autònoma de Barcelona |
| Repositório: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglês |
| OAI Identifier: | oai:ddd.uab.cat:248433 |
| Acesso em linha: | https://ddd.uab.cat/record/248433 https://dx.doi.org/urn:doi:10.1098/rspb.2021.0020 |
| Access Level: | Acceso aberto |
| Palavra-chave: | Dogs Palaeofaeces Palaeoproteomics Zooms Nunalleq Alaska Archaeology |
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Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteomeRunge, Anne Kathrine|||0000-0003-2421-4831Hendy, Jessica|||0000-0002-3718-1058Korzow Richter, Kristine|||0000-0003-3591-6900Masson-MacLean, EdouardBritton, KateMackie, Meaghan|||0000-0003-0763-7592McGrath, Krista Michelle|||0000-0003-0692-1217Collins, Matthew|||0000-0003-4226-5501Cappellini, Enrico|||0000-0001-7885-7811Speller, Camilla|||0000-0001-7128-9903DogsPalaeofaecesPalaeoproteomicsZoomsNunalleq AlaskaArchaeologyThe domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes.Universitat Autònoma de Barcelona. Departament de Prehistòria 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/248433https://dx.doi.org/urn:doi:10.1098/rspb.2021.0020reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengEuropean Commission https://doi.org/10.13039/501100000780 676154open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2484332026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| title |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| spellingShingle |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome Runge, Anne Kathrine|||0000-0003-2421-4831 Dogs Palaeofaeces Palaeoproteomics Zooms Nunalleq Alaska Archaeology |
| title_short |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| title_full |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| title_fullStr |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| title_full_unstemmed |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| title_sort |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
| dc.creator.none.fl_str_mv |
Runge, Anne Kathrine|||0000-0003-2421-4831 Hendy, Jessica|||0000-0002-3718-1058 Korzow Richter, Kristine|||0000-0003-3591-6900 Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan|||0000-0003-0763-7592 McGrath, Krista Michelle|||0000-0003-0692-1217 Collins, Matthew|||0000-0003-4226-5501 Cappellini, Enrico|||0000-0001-7885-7811 Speller, Camilla|||0000-0001-7128-9903 |
| author |
Runge, Anne Kathrine|||0000-0003-2421-4831 |
| author_facet |
Runge, Anne Kathrine|||0000-0003-2421-4831 Hendy, Jessica|||0000-0002-3718-1058 Korzow Richter, Kristine|||0000-0003-3591-6900 Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan|||0000-0003-0763-7592 McGrath, Krista Michelle|||0000-0003-0692-1217 Collins, Matthew|||0000-0003-4226-5501 Cappellini, Enrico|||0000-0001-7885-7811 Speller, Camilla|||0000-0001-7128-9903 |
| author_role |
author |
| author2 |
Hendy, Jessica|||0000-0002-3718-1058 Korzow Richter, Kristine|||0000-0003-3591-6900 Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan|||0000-0003-0763-7592 McGrath, Krista Michelle|||0000-0003-0692-1217 Collins, Matthew|||0000-0003-4226-5501 Cappellini, Enrico|||0000-0001-7885-7811 Speller, Camilla|||0000-0001-7128-9903 |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona. Departament de Prehistòria |
| dc.subject.none.fl_str_mv |
Dogs Palaeofaeces Palaeoproteomics Zooms Nunalleq Alaska Archaeology |
| topic |
Dogs Palaeofaeces Palaeoproteomics Zooms Nunalleq Alaska Archaeology |
| description |
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2 2021-01-01 2021 2021-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/248433 https://dx.doi.org/urn:doi:10.1098/rspb.2021.0020 |
| url |
https://ddd.uab.cat/record/248433 https://dx.doi.org/urn:doi:10.1098/rspb.2021.0020 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
European Commission https://doi.org/10.13039/501100000780 676154 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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