Knee Cartilage and Subchondral Bone Evaluations by Magnetic Resonance Imaging Correlate with Histological Biomarkers in an Osteoarthritis Rabbit Model
Objective. o evaluate pathological changes in cartilage and subchondral bone M biomarkers in a rabbit model of osteoarthritis (O) and correlate these with histological variations. Design. ransection of the anterior cruciate ligament was performed on the right knee of eighteen 12-week-old New Zealand...
| Autores: | , , , , , , |
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| Tipo de documento: | artigo |
| Data de publicação: | 2022 |
| País: | España |
| Recursos: | Universidad Católica de Valencia San Vicente Mártir |
| Repositório: | RIUCV. Repositorio de la Universidad Católica de Valencia San Vicente Mártir |
| Idioma: | inglês |
| OAI Identifier: | oai:riucv.ucv.es:20.500.12466/5015 |
| Acesso em linha: | http://hdl.handle.net/20.500.12466/5015 |
| Access Level: | Acceso aberto |
| Palavra-chave: | 3T MR Biomarkers Cartilage Diagnosis Diagnostics Osteoarthritis Subchondral bone 3109 Ciencias Veterinarias |
| Resumo: | Objective. o evaluate pathological changes in cartilage and subchondral bone M biomarkers in a rabbit model of osteoarthritis (O) and correlate these with histological variations. Design. ransection of the anterior cruciate ligament was performed on the right knee of eighteen 12-week-old New Zealand white rabbits to induce O. 3-esla M images were obtained from 18 healthy control knees (left) and 18 knees with O (right). maging biomarkers included volume, thickness, 1 and 2* cartilage parametric maps, and several subchondral bone features: bone volume to total volume ratio, trabecular thickness, trabecular spacing, trabecular number (bN), 2D and 3D fractal dimensions, and quality of trabecular score (QS). Microscopic analysis of the lateral femoral condyles was set as the ground truth. Results. When healthy and osteoarthritic knees were compared, significant differences were seen in the 1 and 2* values of the femur and tibia cartilage and in the subchondral bone volume to total volume, bN, and QS of both the lateral and medial aspects of the femur and tibia. Histological findings revealed significant osteoarthritic changes between healthy and osteoarthritic knees in stain, structure, chondrocyte density, total score, and subchondral bone biomarker levels. positive correlation was found between histological staining, structure, chondrocyte density, and total score variables in 1 and 2* cartilage biomarkers. negative correlation was observed between histological subchondral bone variables and magnetic resonance D2D and QS biomarkers. Conclusion. Quantification of several cartilage and subchondral bone imaging biomarkers in a rabbit model of O allows the detection of significant changes, which are correlated with histological findings. |
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