Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems

24 p.-8 fig.

Detalles Bibliográficos
Autores: Maestro, Beatriz, Velasco, Isabel, Castillejo, Isabel, Arevalo-Rodriguez, Miguel, Cebolla, Ángel, Sanz, Jesús M.
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/344809
Acceso en línea:http://hdl.handle.net/10261/344809
Access Level:acceso abierto
Palabra clave:Aqueous two-phase systems
Protein purification
Affinity chromatography
Poly(ethylene glycol)
Choline-binding module
Liquid–liquid extraction
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spelling Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systemsMaestro, BeatrizVelasco, IsabelCastillejo, IsabelArevalo-Rodriguez, MiguelCebolla, ÁngelSanz, Jesús M.Aqueous two-phase systemsProtein purificationAffinity chromatographyPoly(ethylene glycol)Choline-binding moduleLiquid–liquid extraction24 p.-8 fig.We present a novel procedure for affinity partitioning of recombinant proteins fused to the choline-binding module C-LytA in aqueous two-phase systems containing poly(ethylene glycol) (PEG). Proteins tagged with the C-LytA module and exposed to the two-phase systems are quantitatively localized in the PEG-rich phase, whereas subsequent addition of the natural ligand choline specifically shifts their localization to the PEG-poor phase by displacement of the polymer from the binding sites. The described procedure is simple, scalable and reproducible, and has been successfully applied to the purification of four diverse proteins, resulting in high yields and purity.This work was partly funded by the Spanish Ministerio de Educación y Ciencia (MEC) (Grants CIT-010000-2005-32 and FIT-010000-2003-110.Peer reviewedElsevierMinisterio de Educación y Ciencia (España)Maestro, Beatriz [0000-0001-5317-650X]Cebolla, Ángel [0000-0002-6976-4522]Sanz, Jesús M. [0000-0002-4421-9376]202420242008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/344809reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.chroma.2008.08.106Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3448092026-05-22T06:33:51Z
dc.title.none.fl_str_mv Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
title Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
spellingShingle Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
Maestro, Beatriz
Aqueous two-phase systems
Protein purification
Affinity chromatography
Poly(ethylene glycol)
Choline-binding module
Liquid–liquid extraction
title_short Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
title_full Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
title_fullStr Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
title_full_unstemmed Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
title_sort Affinity partitioning of proteins tagged with choline-binding modules in aqueous two-phase systems
dc.creator.none.fl_str_mv Maestro, Beatriz
Velasco, Isabel
Castillejo, Isabel
Arevalo-Rodriguez, Miguel
Cebolla, Ángel
Sanz, Jesús M.
author Maestro, Beatriz
author_facet Maestro, Beatriz
Velasco, Isabel
Castillejo, Isabel
Arevalo-Rodriguez, Miguel
Cebolla, Ángel
Sanz, Jesús M.
author_role author
author2 Velasco, Isabel
Castillejo, Isabel
Arevalo-Rodriguez, Miguel
Cebolla, Ángel
Sanz, Jesús M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Educación y Ciencia (España)
Maestro, Beatriz [0000-0001-5317-650X]
Cebolla, Ángel [0000-0002-6976-4522]
Sanz, Jesús M. [0000-0002-4421-9376]
dc.subject.none.fl_str_mv Aqueous two-phase systems
Protein purification
Affinity chromatography
Poly(ethylene glycol)
Choline-binding module
Liquid–liquid extraction
topic Aqueous two-phase systems
Protein purification
Affinity chromatography
Poly(ethylene glycol)
Choline-binding module
Liquid–liquid extraction
description 24 p.-8 fig.
publishDate 2008
dc.date.none.fl_str_mv 2008
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/344809
url http://hdl.handle.net/10261/344809
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.chroma.2008.08.106
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869418107243593728
score 15,811543

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