Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio

In the present work, the volume exclusion phenomenon, also known as macromolecular crowding, has been applied to the field of enzyme kinetics. It has been approached by adding polymeric obstacles in the media of different enzymatic reactions. The concentration and size of these obstacles have been c...

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Autores: Balcells Nadal, Cristina, Pastor, Isabel, Pitulice, Laura, Hernández, Claudia, Via Nadal, Mireia, Garcés, Josep Lluís, Madurga Díez, Sergio, Vilaseca i Font, Eudald, Isvoran, Adriana, Cascante i Serratosa, Marta, Mas i Pujadas, Francesc
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2015
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/158838
Acesso em linha:https://hdl.handle.net/2445/158838
Access Level:Acceso aberto
Palavra-chave:Cinètica enzimàtica
Enzyme kinetics
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spelling Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratioBalcells Nadal, CristinaPastor, IsabelPitulice, LauraHernández, ClaudiaVia Nadal, MireiaGarcés, Josep LluísMadurga Díez, SergioVilaseca i Font, EudaldIsvoran, AdrianaCascante i Serratosa, MartaMas i Pujadas, FrancescCinètica enzimàticaEnzyme kineticsIn the present work, the volume exclusion phenomenon, also known as macromolecular crowding, has been applied to the field of enzyme kinetics. It has been approached by adding polymeric obstacles in the media of different enzymatic reactions. The concentration and size of these obstacles have been changed systematically in order to obtain kinetic information about each reaction. Results indicate that the performance of a certain enzyme always depends on the amount of excluded volume. However, only large, oligomeric proteins display an obstacle size-dependent behavior. Besides, crowding can hinder diffusion to the extent of being capable of shifting reaction control from activation to diffusion.Universitatea de Vest din Timişoara2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/158838Articles publicats en revistes (Ciència dels Materials i Química Física)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://newfrontchem.iqstorm.ro/New Frontiers in Chemistry, 2015, vol. 24, num. 1, p. 3-16(c) Universitatea de Vest din Timişoara, 2015info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1588382026-05-27T06:46:51Z
dc.title.none.fl_str_mv Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
title Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
spellingShingle Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
Balcells Nadal, Cristina
Cinètica enzimàtica
Enzyme kinetics
title_short Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
title_full Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
title_fullStr Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
title_full_unstemmed Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
title_sort Macromolecular crowding upon in-vivo-like enzyme-kinetics: effect of enzyme-obstacle size ratio
dc.creator.none.fl_str_mv Balcells Nadal, Cristina
Pastor, Isabel
Pitulice, Laura
Hernández, Claudia
Via Nadal, Mireia
Garcés, Josep Lluís
Madurga Díez, Sergio
Vilaseca i Font, Eudald
Isvoran, Adriana
Cascante i Serratosa, Marta
Mas i Pujadas, Francesc
author Balcells Nadal, Cristina
author_facet Balcells Nadal, Cristina
Pastor, Isabel
Pitulice, Laura
Hernández, Claudia
Via Nadal, Mireia
Garcés, Josep Lluís
Madurga Díez, Sergio
Vilaseca i Font, Eudald
Isvoran, Adriana
Cascante i Serratosa, Marta
Mas i Pujadas, Francesc
author_role author
author2 Pastor, Isabel
Pitulice, Laura
Hernández, Claudia
Via Nadal, Mireia
Garcés, Josep Lluís
Madurga Díez, Sergio
Vilaseca i Font, Eudald
Isvoran, Adriana
Cascante i Serratosa, Marta
Mas i Pujadas, Francesc
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cinètica enzimàtica
Enzyme kinetics
topic Cinètica enzimàtica
Enzyme kinetics
description In the present work, the volume exclusion phenomenon, also known as macromolecular crowding, has been applied to the field of enzyme kinetics. It has been approached by adding polymeric obstacles in the media of different enzymatic reactions. The concentration and size of these obstacles have been changed systematically in order to obtain kinetic information about each reaction. Results indicate that the performance of a certain enzyme always depends on the amount of excluded volume. However, only large, oligomeric proteins display an obstacle size-dependent behavior. Besides, crowding can hinder diffusion to the extent of being capable of shifting reaction control from activation to diffusion.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/158838
url https://hdl.handle.net/2445/158838
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://newfrontchem.iqstorm.ro/
New Frontiers in Chemistry, 2015, vol. 24, num. 1, p. 3-16
dc.rights.none.fl_str_mv (c) Universitatea de Vest din Timişoara, 2015
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Universitatea de Vest din Timişoara, 2015
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universitatea de Vest din Timişoara
publisher.none.fl_str_mv Universitatea de Vest din Timişoara
dc.source.none.fl_str_mv Articles publicats en revistes (Ciència dels Materials i Química Física)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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