Integration of actomyosin contractility with cell-cell adhesion during dorsal closure

In this work, we combine genetic perturbation, time-lapse imaging and quantitative image analysis to investigate how pulsatile actomyosin contractility drives cell oscillations, apical cell contraction and tissue closure during morphogenesis of the amnioserosa, the main force-generating tissue durin...

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Detalhes bibliográficos
Autores: Duque, Julia, Gorfinkiel, Nicole
Formato: artículo
Fecha de publicación:2016
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/151669
Acesso em linha:http://hdl.handle.net/10261/151669
Access Level:acceso abierto
Palavra-chave:Oscillations
Apical contraction
Actomyosin
Adhesion
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spelling Integration of actomyosin contractility with cell-cell adhesion during dorsal closureDuque, JuliaGorfinkiel, NicoleOscillationsApical contractionActomyosinAdhesionIn this work, we combine genetic perturbation, time-lapse imaging and quantitative image analysis to investigate how pulsatile actomyosin contractility drives cell oscillations, apical cell contraction and tissue closure during morphogenesis of the amnioserosa, the main force-generating tissue during the dorsal closure in Drosophila. We show that Myosin activity determines the oscillatory and contractile behaviour of amnioserosa cells. Reducing Myosin activity prevents cell shape oscillations and reduces cell contractility. By contrast, increasing Myosin activity increases the amplitude of cell shape oscillations and the time cells spend in the contracted phase relative to the expanded phase during an oscillatory cycle, promoting cell contractility and tissue closure. Furthermore, we show that in AS cells, Rok controls Myosin foci formation and Mbs regulates not only Myosin phosphorylation but also adhesion dynamics through control of Moesin phosphorylation, showing that Mbs coordinates actomyosin contractility with cell-cell adhesion during amnioserosa morphogenesis.Ministerio de Ciencia e Innovación (BFU-2011-25828 and ‘Ramón y Cajal’ fellowship award) and a European Commission Marie Curie Career Integration Grant (PCIG09-GA-2011-293479). J.D. is a recipient of a PhD FPI-fellowship (BES-2012-051839) from the Ministerio de Ciencia e InnovaciónPeer ReviewedCompany of BiologistsMinisterio de Ciencia e Innovación (España)European CommissionMinisterio de Ciencia e Innovación (España)2017201720162017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/151669reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1516692026-05-22T06:33:51Z
dc.title.none.fl_str_mv Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
title Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
spellingShingle Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
Duque, Julia
Oscillations
Apical contraction
Actomyosin
Adhesion
title_short Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
title_full Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
title_fullStr Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
title_full_unstemmed Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
title_sort Integration of actomyosin contractility with cell-cell adhesion during dorsal closure
dc.creator.none.fl_str_mv Duque, Julia
Gorfinkiel, Nicole
author Duque, Julia
author_facet Duque, Julia
Gorfinkiel, Nicole
author_role author
author2 Gorfinkiel, Nicole
author2_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Ministerio de Ciencia e Innovación (España)
dc.subject.none.fl_str_mv Oscillations
Apical contraction
Actomyosin
Adhesion
topic Oscillations
Apical contraction
Actomyosin
Adhesion
description In this work, we combine genetic perturbation, time-lapse imaging and quantitative image analysis to investigate how pulsatile actomyosin contractility drives cell oscillations, apical cell contraction and tissue closure during morphogenesis of the amnioserosa, the main force-generating tissue during the dorsal closure in Drosophila. We show that Myosin activity determines the oscillatory and contractile behaviour of amnioserosa cells. Reducing Myosin activity prevents cell shape oscillations and reduces cell contractility. By contrast, increasing Myosin activity increases the amplitude of cell shape oscillations and the time cells spend in the contracted phase relative to the expanded phase during an oscillatory cycle, promoting cell contractility and tissue closure. Furthermore, we show that in AS cells, Rok controls Myosin foci formation and Mbs regulates not only Myosin phosphorylation but also adhesion dynamics through control of Moesin phosphorylation, showing that Mbs coordinates actomyosin contractility with cell-cell adhesion during amnioserosa morphogenesis.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/151669
url http://hdl.handle.net/10261/151669
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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