Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice

Aim: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected. Background: Bacteriophage is of particular interest as a biocontrol agent in the prevention of fo...

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Autores: Nikkhahi, Farhad, Dallal, Mohammad Mehdi Soltan, Alimohammadi, Mahmood, Foroushani, Abbas Rahimi, Rajabi, Zahra, Fardsanei, Fatemeh, Bonab, Parisa Torabi, Mostafa Imeni, Seyed
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:UVic-UCC
Repositorio:RiUVic. Repositori institucional de la UVic-UCC
OAI Identifier:oai:dspace.uvic.cat:10854/5021
Acceso en línea:http://hdl.handle.net/10854/5021
https://doi.org/10.22037/ghfbb.v0i0.1003
Access Level:acceso abierto
Palabra clave:Bacteriòfags
Salmonel·la
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spelling Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in miceNikkhahi, FarhadDallal, Mohammad Mehdi SoltanAlimohammadi, MahmoodForoushani, Abbas RahimiRajabi, ZahraFardsanei, FatemehBonab, Parisa TorabiMostafa Imeni, SeyedBacteriòfagsSalmonel·laAim: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected. Background: Bacteriophage is of particular interest as a biocontrol agent in the prevention of food-borne illnesses. In recent years tend to use bacteriophages to control pathogenic bacteria has increased. A bacteriophage is considered to be a potent antibiotic alternative for treating bacterial infections. Methods: the specific phages against Salmonella Enteritidis was isolated and candidates for therapy of mice will be selected. Mouses divided into the six specific groups. Groups of mice were as follows: A: Bacteri (control) B: Bacteri+ bacteriophage (Simultaneous), C: Bacteri + bacteriophage Four days later, D: Bacteriophage + bacteri four days later E: Bacteri+ Ciprofloxacin (Simultaneous) F: Bacteri+ ciprofloxacin+ bacteriophage (Simultaneous). Results: In this study, a lytic bacteriophage is isolated and it shows that phage has a head size of 46 nm and without a tail, by using an electron microscope. Oral administration of a single dose of 2 × 109 PFU/mouse bacteriophage enable to protect mouse against salmonellosis and it causes treatment of salmonellosis in mice. Conclusion: The use of this phage compared to ciprofloxacin shows that in addition of the treatment of mouse, it also prevents weight loss.Research Institute for Gastroenterology and Liver Diseases2017201720172017info:eu-repo/semantics/articleinfo:eu-repo/publishedVersionapplication/pdf6 p.application/pdfhttp://hdl.handle.net/10854/5021https://doi.org/10.22037/ghfbb.v0i0.1003reponame:RiUVic. Repositori institucional de la UVic-UCCinstname:UVic-UCCInglésAquest document està subjecte a aquesta llicència Creative Commonshttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:dspace.uvic.cat:10854/50212026-06-07T19:15:21Z
dc.title.none.fl_str_mv Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
title Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
spellingShingle Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
Nikkhahi, Farhad
Bacteriòfags
Salmonel·la
title_short Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
title_full Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
title_fullStr Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
title_full_unstemmed Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
title_sort Phage therapy: assessment of the efficacy of a bacteriophage isolated in the treatment of salmonellosis induced by Salmonella enteritidis in mice
dc.creator.none.fl_str_mv Nikkhahi, Farhad
Dallal, Mohammad Mehdi Soltan
Alimohammadi, Mahmood
Foroushani, Abbas Rahimi
Rajabi, Zahra
Fardsanei, Fatemeh
Bonab, Parisa Torabi
Mostafa Imeni, Seyed
author Nikkhahi, Farhad
author_facet Nikkhahi, Farhad
Dallal, Mohammad Mehdi Soltan
Alimohammadi, Mahmood
Foroushani, Abbas Rahimi
Rajabi, Zahra
Fardsanei, Fatemeh
Bonab, Parisa Torabi
Mostafa Imeni, Seyed
author_role author
author2 Dallal, Mohammad Mehdi Soltan
Alimohammadi, Mahmood
Foroushani, Abbas Rahimi
Rajabi, Zahra
Fardsanei, Fatemeh
Bonab, Parisa Torabi
Mostafa Imeni, Seyed
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bacteriòfags
Salmonel·la
topic Bacteriòfags
Salmonel·la
description Aim: This work aims to isolate and perform comparative studies of a phages active against a Salmonella enteritidis strain from Iran. Also, suitable phage candidates for therapy of mice will be selected. Background: Bacteriophage is of particular interest as a biocontrol agent in the prevention of food-borne illnesses. In recent years tend to use bacteriophages to control pathogenic bacteria has increased. A bacteriophage is considered to be a potent antibiotic alternative for treating bacterial infections. Methods: the specific phages against Salmonella Enteritidis was isolated and candidates for therapy of mice will be selected. Mouses divided into the six specific groups. Groups of mice were as follows: A: Bacteri (control) B: Bacteri+ bacteriophage (Simultaneous), C: Bacteri + bacteriophage Four days later, D: Bacteriophage + bacteri four days later E: Bacteri+ Ciprofloxacin (Simultaneous) F: Bacteri+ ciprofloxacin+ bacteriophage (Simultaneous). Results: In this study, a lytic bacteriophage is isolated and it shows that phage has a head size of 46 nm and without a tail, by using an electron microscope. Oral administration of a single dose of 2 × 109 PFU/mouse bacteriophage enable to protect mouse against salmonellosis and it causes treatment of salmonellosis in mice. Conclusion: The use of this phage compared to ciprofloxacin shows that in addition of the treatment of mouse, it also prevents weight loss.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/publishedVersion
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10854/5021
https://doi.org/10.22037/ghfbb.v0i0.1003
url http://hdl.handle.net/10854/5021
https://doi.org/10.22037/ghfbb.v0i0.1003
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Aquest document està subjecte a aquesta llicència Creative Commons
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Aquest document està subjecte a aquesta llicència Creative Commons
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
6 p.
application/pdf
dc.publisher.none.fl_str_mv Research Institute for Gastroenterology and Liver Diseases
publisher.none.fl_str_mv Research Institute for Gastroenterology and Liver Diseases
dc.source.none.fl_str_mv reponame:RiUVic. Repositori institucional de la UVic-UCC
instname:UVic-UCC
instname_str UVic-UCC
reponame_str RiUVic. Repositori institucional de la UVic-UCC
collection RiUVic. Repositori institucional de la UVic-UCC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300724

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