Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells

Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinf...

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Detalles Bibliográficos
Autores: Ruiz Yance, Iris, Siguas, Junior, Bardales, Brandy, Robles Castañeda, Ingrid, Cordova, Karen, Ypushima, Alina, Estela Villar, Esteban, Quintana Criollo, Carlos, Estacio, Darwin, Rodríguez, José Luis
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/104025
Acceso en línea:https://hdl.handle.net/20.500.14352/104025
Access Level:acceso abierto
Palabra clave:615
Cytotoxicity
EA.hy926
Ipconazole fungicide
Oxidative stress
Proinflammation
Farmacología (Farmacia)
3209 Farmacología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/104025
network_acronym_str ES
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repository_id_str
spelling Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like CellsRuiz Yance, IrisSiguas, JuniorBardales, BrandyRobles Castañeda, IngridCordova, KarenYpushima, AlinaEstela Villar, EstebanQuintana Criollo, CarlosEstacio, DarwinRodríguez, José Luis615CytotoxicityEA.hy926Ipconazole fungicideOxidative stressProinflammationFarmacología (Farmacia)3209 FarmacologíaTriazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinflammation of EA.hy926 endothelial cells in response to ipconazole exposure. Using the MTT assay, ipconazole was found to produce a dose-dependent reduction (*** p < 0.001; concentrations of 20, 50 and 100 µM) of cell viability in EA.hy926 with an IC50 of 29 µM. Also, ipconazole induced a significant increase in ROS generation (** p < 0.01), caspase 3/7 (** p < 0.01), cell death (BAX, APAF1, BNIP3, CASP3 and AKT1) and proinflammatory (NLRP3, CASP1, IL1β, NFκB, IL6 and TNFα) biomarkers, as well as a reduction in antioxidant (NRF2 and GPx) biomarkers. These results demonstrated that oxidative stress, proinflammatory activity and cell death could be responsible for the cytotoxic effect produced by the fungicide ipconazole, such that this triazole compound should be considered as a possible risk factor in the development of alterations in cellular homeostasis.MDPIUniversidad Complutense de Madrid20232023-10-0520232023-10-05journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/104025reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1040252026-06-02T12:44:21Z
dc.title.none.fl_str_mv Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
title Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
spellingShingle Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
Ruiz Yance, Iris
615
Cytotoxicity
EA.hy926
Ipconazole fungicide
Oxidative stress
Proinflammation
Farmacología (Farmacia)
3209 Farmacología
title_short Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
title_full Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
title_fullStr Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
title_full_unstemmed Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
title_sort Potential Involvement of Oxidative Stress, Apoptosis and Proinflammation in Ipconazole-Induced Cytotoxicity in Human Endothelial-like Cells
dc.creator.none.fl_str_mv Ruiz Yance, Iris
Siguas, Junior
Bardales, Brandy
Robles Castañeda, Ingrid
Cordova, Karen
Ypushima, Alina
Estela Villar, Esteban
Quintana Criollo, Carlos
Estacio, Darwin
Rodríguez, José Luis
author Ruiz Yance, Iris
author_facet Ruiz Yance, Iris
Siguas, Junior
Bardales, Brandy
Robles Castañeda, Ingrid
Cordova, Karen
Ypushima, Alina
Estela Villar, Esteban
Quintana Criollo, Carlos
Estacio, Darwin
Rodríguez, José Luis
author_role author
author2 Siguas, Junior
Bardales, Brandy
Robles Castañeda, Ingrid
Cordova, Karen
Ypushima, Alina
Estela Villar, Esteban
Quintana Criollo, Carlos
Estacio, Darwin
Rodríguez, José Luis
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 615
Cytotoxicity
EA.hy926
Ipconazole fungicide
Oxidative stress
Proinflammation
Farmacología (Farmacia)
3209 Farmacología
topic 615
Cytotoxicity
EA.hy926
Ipconazole fungicide
Oxidative stress
Proinflammation
Farmacología (Farmacia)
3209 Farmacología
description Triazole fungicides are widely used in the world, mainly in agriculture, but their abuse and possible toxic effects are being reported in some in vivo and in vitro studies that have demonstrated their danger to human health. This in vitro study evaluated the cytotoxicity, oxidative stress and proinflammation of EA.hy926 endothelial cells in response to ipconazole exposure. Using the MTT assay, ipconazole was found to produce a dose-dependent reduction (*** p < 0.001; concentrations of 20, 50 and 100 µM) of cell viability in EA.hy926 with an IC50 of 29 µM. Also, ipconazole induced a significant increase in ROS generation (** p < 0.01), caspase 3/7 (** p < 0.01), cell death (BAX, APAF1, BNIP3, CASP3 and AKT1) and proinflammatory (NLRP3, CASP1, IL1β, NFκB, IL6 and TNFα) biomarkers, as well as a reduction in antioxidant (NRF2 and GPx) biomarkers. These results demonstrated that oxidative stress, proinflammatory activity and cell death could be responsible for the cytotoxic effect produced by the fungicide ipconazole, such that this triazole compound should be considered as a possible risk factor in the development of alterations in cellular homeostasis.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-10-05
2023
2023-10-05
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/104025
url https://hdl.handle.net/20.500.14352/104025
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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