Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification

[EN] Magnetic-chitosan particles were prepared following three different protocols enabling the preparation of particles with different sizes – nano (Nano-CMag, Micro (Micro-CMag) and Macro (Macro-CMag) – and used for pectinase immobilization and clarification of grape, apple and orange juices. The...

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Autores: Dal-Magro,Lucas, Silva de Moura, Kelly, Backes, Betina Elys, de-Menezes,Eliana Weber, Benvenutti, Edilson Valmir, Nicolodi, Sabrina, Klein, Manuela P., Fernández-Lafuente, Roberto, Rodrigues, Rafael C.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/209875
Acceso en línea:http://hdl.handle.net/10261/209875
Access Level:acceso abierto
Palabra clave:Chitosan
Magnetic particles
Pectin
Pectinase
Immobilization
Juice clarification
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spelling Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarificationDal-Magro,LucasSilva de Moura, KellyBackes, Betina Elysde-Menezes,Eliana WeberBenvenutti, Edilson ValmirNicolodi, SabrinaKlein, Manuela P.Fernández-Lafuente, RobertoRodrigues, Rafael C.ChitosanMagnetic particlesPectinPectinaseImmobilizationJuice clarification[EN] Magnetic-chitosan particles were prepared following three different protocols enabling the preparation of particles with different sizes – nano (Nano-CMag, Micro (Micro-CMag) and Macro (Macro-CMag) – and used for pectinase immobilization and clarification of grape, apple and orange juices. The particle size had a great effect in the kinetic parameters, Nano-CMag biocatalyst presented the highest V value (78.95 mg. min), followed by Micro-CMag and Macro-CMag, with V of 57.20 mg.min and 46.03 mg.min, respectively. However, the highest thermal stability was achieved using Macro-CMag, that was 8 and 3-times more stable than Nano-CMag and Micro-CMag biocatalysts, respectively. Pectinase immobilized on Macro-CMag kept 85% of its initial activity after 25 batch cycles in orange juice clarification. These results suggested that the chitosan magnetic biocatalysts presented great potential application as clarifying catalysts for the fruit juice industry and the great importance of the chitosan particles preparation on the final biocatalyst properties.This work was supported by grants and scholarships (L Dal Magro) from Capes, CNPq (process 403505/2013-5) and FAPERGS (process 17/2551-0000939-8). We gratefully recognize the support from the MINECO from Spanish Government, (project number CTQ2017-86170-R) The authors wish to thank Mr. Ramiro Martínez (Novozymes, Spain), Amazon group and LNF Latinoamericana for kindly supplying the enzymes used in this research, as well as Vitivinicola Jolimont (Canela, Brazil) for providing the grape juice.ElsevierMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/209875reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-86170-Rhttp://dx.doi.org/10.1016/j.btre.2019.e00373Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2098752026-05-22T06:33:51Z
dc.title.none.fl_str_mv Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
title Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
spellingShingle Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
Dal-Magro,Lucas
Chitosan
Magnetic particles
Pectin
Pectinase
Immobilization
Juice clarification
title_short Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
title_full Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
title_fullStr Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
title_full_unstemmed Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
title_sort Immobilization of pectinase on chitosan-magnetic particles: Influence of particle preparation protocol on enzyme properties for fruit juice clarification
dc.creator.none.fl_str_mv Dal-Magro,Lucas
Silva de Moura, Kelly
Backes, Betina Elys
de-Menezes,Eliana Weber
Benvenutti, Edilson Valmir
Nicolodi, Sabrina
Klein, Manuela P.
Fernández-Lafuente, Roberto
Rodrigues, Rafael C.
author Dal-Magro,Lucas
author_facet Dal-Magro,Lucas
Silva de Moura, Kelly
Backes, Betina Elys
de-Menezes,Eliana Weber
Benvenutti, Edilson Valmir
Nicolodi, Sabrina
Klein, Manuela P.
Fernández-Lafuente, Roberto
Rodrigues, Rafael C.
author_role author
author2 Silva de Moura, Kelly
Backes, Betina Elys
de-Menezes,Eliana Weber
Benvenutti, Edilson Valmir
Nicolodi, Sabrina
Klein, Manuela P.
Fernández-Lafuente, Roberto
Rodrigues, Rafael C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Chitosan
Magnetic particles
Pectin
Pectinase
Immobilization
Juice clarification
topic Chitosan
Magnetic particles
Pectin
Pectinase
Immobilization
Juice clarification
description [EN] Magnetic-chitosan particles were prepared following three different protocols enabling the preparation of particles with different sizes – nano (Nano-CMag, Micro (Micro-CMag) and Macro (Macro-CMag) – and used for pectinase immobilization and clarification of grape, apple and orange juices. The particle size had a great effect in the kinetic parameters, Nano-CMag biocatalyst presented the highest V value (78.95 mg. min), followed by Micro-CMag and Macro-CMag, with V of 57.20 mg.min and 46.03 mg.min, respectively. However, the highest thermal stability was achieved using Macro-CMag, that was 8 and 3-times more stable than Nano-CMag and Micro-CMag biocatalysts, respectively. Pectinase immobilized on Macro-CMag kept 85% of its initial activity after 25 batch cycles in orange juice clarification. These results suggested that the chitosan magnetic biocatalysts presented great potential application as clarifying catalysts for the fruit juice industry and the great importance of the chitosan particles preparation on the final biocatalyst properties.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/209875
url http://hdl.handle.net/10261/209875
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/CTQ2017-86170-R
http://dx.doi.org/10.1016/j.btre.2019.e00373
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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