RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity

14 p.-4 fig.-3 tab.

Detalles Bibliográficos
Autores: Vidal Quist, José Cristian, Declercq, Jozefien, Vanhee, Stijn, Lambrecht, Bart N., Gómez-Rial, José, Vidal, Carmen, Aydogdu, Eylem, Rombauts, Stéphane, Hernández-Crespo, Pedro
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/285175
Acceso en línea:http://hdl.handle.net/10261/285175
Access Level:acceso abierto
Palabra clave:Allergen
Dermatophagoides
RNA virus
House dust mites
Physiology
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spelling RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicityVidal Quist, José CristianDeclercq, JozefienVanhee, StijnLambrecht, Bart N.Gómez-Rial, JoséVidal, CarmenAydogdu, EylemRombauts, StéphaneHernández-Crespo, PedroAllergenDermatophagoidesRNA virusHouse dust mitesPhysiology14 p.-4 fig.-3 tab.RNA viruses have recently been detected in association with house dust mites, including laboratory cultures, dust samples, and mite-derived pharmaceuticals used for allergy diagnosis. The aim of this study is to assess the incidence of viral infection on Dermatophagoides pteronyssinus physiology and on the allergenic performance of extracts derived from its culture. Transcriptional changes between genetically identical control and virus-infected mite colonies were analyzed by RNAseq with the support of a new D. pteronyssinus high-quality annotated genome (56.8 Mb, 108 scaffolds, N50=2.73 Mb, 96.7% BUSCO-completeness). Extracts of cultures and bodies from both colonies were compared by inspecting major allergen accumulation by ELISA, allergen-related enzymatic activities by specific assays, airway inflammation in a mouse model of allergic asthma, and binding to allergic patient's sera IgE by ImmunoCAP. Viral infection induced a significant transcriptional response, including several immunity and stress-response genes, and affected the expression of seven allergens, putative isoallergens and allergen orthologs. Major allergens were unaffected except for Der p 23 that was upregulated, increasing ELISA titers up to 29% in infected-mite extracts. By contrast, serine protease allergens Der p 3, 6 and 9 were downregulated, being trypsin and chymotrypsin enzymatic activities reduced up to 21% in extracts. None of the parameters analyzed in our mouse model, nor binding to human IgE were significantly different when comparing control and infected-mite extracts. Despite the described physiological impact of viral infection on the mites, no significant consequences for the allergenicity of derived extracts or their practical use in allergy diagnosis have been detected. This article is protected by copyright. All rights reserved.SV is supported by a senior postdoctoral fellowship from FWO Flanders (grant 1244321N), JD is supported by a doctoral fellowship from FWO Flanders (grant 11B7720N), and BNL received a European Research Council Advanced Grant (ERC-2017-ADG-789384), as well as a University of Ghent Methusalem Grant.Peer reviewedJohn Wiley & SonsResearch Foundation - FlandersEuropean CommissionGhent UniversityVidal Quist, José Cristian [0000-0002-3931-2592]Vanhee, Stijn [0000-0001-5305-1651]Lambrecht, Bart N. [0000-0003-4376-6834]Gómez-Rial, José [0000-0002-3173-8484]Vidal, Carmen [0000-0002-2844-2754]Rombauts, Stéphane [0000-0002-3985-4981]Hernández-Crespo, Pedro [0000-0001-9350-3640]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/285175reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1111/imb.12822Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2851752026-05-22T06:33:51Z
dc.title.none.fl_str_mv RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
title RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
spellingShingle RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
Vidal Quist, José Cristian
Allergen
Dermatophagoides
RNA virus
House dust mites
Physiology
title_short RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
title_full RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
title_fullStr RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
title_full_unstemmed RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
title_sort RNA viruses alter house dust mite physiology and allergen production with no detected consequences for allergenicity
dc.creator.none.fl_str_mv Vidal Quist, José Cristian
Declercq, Jozefien
Vanhee, Stijn
Lambrecht, Bart N.
Gómez-Rial, José
Vidal, Carmen
Aydogdu, Eylem
Rombauts, Stéphane
Hernández-Crespo, Pedro
author Vidal Quist, José Cristian
author_facet Vidal Quist, José Cristian
Declercq, Jozefien
Vanhee, Stijn
Lambrecht, Bart N.
Gómez-Rial, José
Vidal, Carmen
Aydogdu, Eylem
Rombauts, Stéphane
Hernández-Crespo, Pedro
author_role author
author2 Declercq, Jozefien
Vanhee, Stijn
Lambrecht, Bart N.
Gómez-Rial, José
Vidal, Carmen
Aydogdu, Eylem
Rombauts, Stéphane
Hernández-Crespo, Pedro
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Research Foundation - Flanders
European Commission
Ghent University
Vidal Quist, José Cristian [0000-0002-3931-2592]
Vanhee, Stijn [0000-0001-5305-1651]
Lambrecht, Bart N. [0000-0003-4376-6834]
Gómez-Rial, José [0000-0002-3173-8484]
Vidal, Carmen [0000-0002-2844-2754]
Rombauts, Stéphane [0000-0002-3985-4981]
Hernández-Crespo, Pedro [0000-0001-9350-3640]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Allergen
Dermatophagoides
RNA virus
House dust mites
Physiology
topic Allergen
Dermatophagoides
RNA virus
House dust mites
Physiology
description 14 p.-4 fig.-3 tab.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/285175
url http://hdl.handle.net/10261/285175
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1111/imb.12822
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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