Conformational behavior, topographical features, and antioxidant activity of partly de-esterified arabinoxylans

This study aimed to investigate the effect of arabinoxylans (AX) partial de-esterification with feruloyl esterase on the polysaccharide conformational behavior, topographical features, and antioxidant activity. After enzyme treatment, the ferulic acid (FA) content in AX was reduced from 7.30 to 5.48...

Descripción completa

Detalles Bibliográficos
Autores: Anda-Flores, Y.D. (Yubia De)|||/items/649bd5cd-ff7a-4271-8a1e-13a3b069c54b, Carvajal-Millan, E. (Elizabeth)|||/items/65b195d8-6e00-40cc-982c-5e12314a8e35, Lizardi-Mendoza, J. (Jaime)|||/items/9776ff6d-0705-4958-bfe9-85a944a5089f, Rascon-Chu, A. (Augustin)|||/items/3d46ef0e-5ff7-4e15-8d3e-2d75f7160356, Tanori-Cordova, J. (Judith)|||/items/d67290dc-7772-4f8a-940d-bb42835d6c0f, Martínez-López, A.L. (Ana Luisa)|||/items/c09a6a4f-fa01-4f50-b224-ad0ce9731cea, Burgara-Estrella, A.J. (Alexel J.)|||/items/611bbd56-8b69-4164-afe0-3c432314a440, Pedroza-Montero, M.R. (Martin R.)|||/items/88882aa0-62ac-4f1d-bae6-5b4a0c0982f9
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/114119
Acceso en línea:https://hdl.handle.net/10171/114119
Access Level:acceso abierto
Palabra clave:Arabinoxylan
Ferulic acid
Macromolecular characteristics
Partial de-esterification
Descripción
Sumario:This study aimed to investigate the effect of arabinoxylans (AX) partial de-esterification with feruloyl esterase on the polysaccharide conformational behavior, topographical features, and antioxidant activity. After enzyme treatment, the ferulic acid (FA) content in AX was reduced from 7.30 to 5.48 µg FA/mg polysaccharide, and the molecule registered a small reduction in radius of gyration (RG), hydrodynamic radius (Rh), characteristic ratio (C∞), and persistence length (q). A slight decrease in α and a small increase in K constants in the Mark-Houwink-Sakurada equation for partially de-esterified AX (FAX) suggested a reduction in molecule structural rigidity and a more expanded coil conformation, respectively, in relation to AX. Fourier transform infrared spectroscopy spectra of AX and FAX presented a pattern characteristic for this polysaccharide. Atomic force microscopy topographic analysis of FAX showed a more regular surface without larger hollows in relation to AX. The antioxidant activity of FAX, compared to AX, was reduced by 30 and 41% using both 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS+) and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) methods, respectively. These results suggest that feruloyl esterase treatment of AX could offer a strategy to tailor AX chains conformation, morphological features, and antioxidant activity, impacting the development of advanced biomaterials for biomedical and pharmaceutical applications.

Similares en LA Referencia