Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus

The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Si...

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Detalles Bibliográficos
Autores: Jiménez-Zaragoza, Manuel, Yubero, Marina Pl, Martin-Forero, Esther, Castón, José R, Reguera, David, Luque, Daniel, de Pablo, Pedro J, Rodriguez Martinez, Javier M
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/9772
Acceso en línea:http://hdl.handle.net/20.500.12105/9772
Access Level:acceso abierto
Palabra clave:Finite Element Analysis
Microscopy, Atomic Force
Models, Biological
Nanoparticles
Rotavirus
Viral Proteins
Virion
Biophysical Phenomena
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spelling Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virusJiménez-Zaragoza, ManuelYubero, Marina PlMartin-Forero, EstherCastón, José RReguera, DavidLuque, Danielde Pablo, Pedro JRodriguez Martinez, Javier MFinite Element AnalysisMicroscopy, Atomic ForceModels, BiologicalNanoparticlesRotavirusViral ProteinsVirionBiophysical PhenomenaThe functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion.eLife Sciences PublicationsMinisterio de Economía y Competitividad (España)Comunidad de Madrid (España)20202020-04-2820182018-01-0120182018-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/20.500.12105/9772reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/97722026-06-12T12:43:37Z
dc.title.none.fl_str_mv Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
title Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
spellingShingle Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
Jiménez-Zaragoza, Manuel
Finite Element Analysis
Microscopy, Atomic Force
Models, Biological
Nanoparticles
Rotavirus
Viral Proteins
Virion
Biophysical Phenomena
title_short Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
title_full Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
title_fullStr Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
title_full_unstemmed Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
title_sort Biophysical properties of single rotavirus particles account for the functions of protein shells in a multilayered virus
dc.creator.none.fl_str_mv Jiménez-Zaragoza, Manuel
Yubero, Marina Pl
Martin-Forero, Esther
Castón, José R
Reguera, David
Luque, Daniel
de Pablo, Pedro J
Rodriguez Martinez, Javier M
author Jiménez-Zaragoza, Manuel
author_facet Jiménez-Zaragoza, Manuel
Yubero, Marina Pl
Martin-Forero, Esther
Castón, José R
Reguera, David
Luque, Daniel
de Pablo, Pedro J
Rodriguez Martinez, Javier M
author_role author
author2 Yubero, Marina Pl
Martin-Forero, Esther
Castón, José R
Reguera, David
Luque, Daniel
de Pablo, Pedro J
Rodriguez Martinez, Javier M
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Comunidad de Madrid (España)
dc.subject.none.fl_str_mv Finite Element Analysis
Microscopy, Atomic Force
Models, Biological
Nanoparticles
Rotavirus
Viral Proteins
Virion
Biophysical Phenomena
topic Finite Element Analysis
Microscopy, Atomic Force
Models, Biological
Nanoparticles
Rotavirus
Viral Proteins
Virion
Biophysical Phenomena
description The functions performed by the concentric shells of multilayered dsRNA viruses require specific protein interactions that can be directly explored through their mechanical properties. We studied the stiffness, breaking force, critical strain and mechanical fatigue of individual Triple, Double and Single layered rotavirus (RV) particles. Our results, in combination with Finite Element simulations, demonstrate that the mechanics of the external layer provides the resistance needed to counteract the stringent conditions of extracellular media. Our experiments, in combination with electrostatic analyses, reveal a strong interaction between the two outer layers and how it is suppressed by the removal of calcium ions, a key step for transcription initiation. The intermediate layer presents weak hydrophobic interactions with the inner layer that allow the assembly and favor the conformational dynamics needed for transcription. Our work shows how the biophysical properties of the three shells are finely tuned to produce an infective RV virion.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01
2018
2018-01-01
2020
2020-04-28
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12105/9772
url http://hdl.handle.net/20.500.12105/9772
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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