Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue

New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle. Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride. However, it is difficult to o...

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Autores: García Lizarribar, Andrea, Fernández Garibay, Xiomara, Velasco Mallorquí, Ferran, García Castaño, F. Javier, Samitier i Martí, Josep, Ramon Azcon, Javier
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/125984
Acceso en línea:https://hdl.handle.net/2445/125984
Access Level:acceso abierto
Palabra clave:Materials biomèdics
Impressió 3D
Teixits (Histologia)
Biomedical materials
Three-dimensional printing
Tissues
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spelling Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissueGarcía Lizarribar, AndreaFernández Garibay, XiomaraVelasco Mallorquí, FerranGarcía Castaño, F. JavierSamitier i Martí, JosepRamon Azcon, JavierMaterials biomèdicsImpressió 3DTeixits (Histologia)Biomedical materialsThree-dimensional printingTissuesNew biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle. Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride. However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering. In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures. In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented. Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends. Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA. All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks. Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.2018info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2445/125984Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: http://dx.doi.org/10.1002/mabi.201800167Macromolecular Bioscience, 2018, vol. 18, num. 10, p. 1800167https://doi.org/10.1002/mabi.201800167info:eu-repo/grantAgreement/EC/H2020/714317(c) Wiley-VCH, 2018info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1259842026-05-27T06:46:51Z
dc.title.none.fl_str_mv Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
title Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
spellingShingle Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
García Lizarribar, Andrea
Materials biomèdics
Impressió 3D
Teixits (Histologia)
Biomedical materials
Three-dimensional printing
Tissues
title_short Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
title_full Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
title_fullStr Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
title_full_unstemmed Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
title_sort Composite biomaterials as long-lasting scaffolds for 3D bioprinting of highly aligned muscle tissue
dc.creator.none.fl_str_mv García Lizarribar, Andrea
Fernández Garibay, Xiomara
Velasco Mallorquí, Ferran
García Castaño, F. Javier
Samitier i Martí, Josep
Ramon Azcon, Javier
author García Lizarribar, Andrea
author_facet García Lizarribar, Andrea
Fernández Garibay, Xiomara
Velasco Mallorquí, Ferran
García Castaño, F. Javier
Samitier i Martí, Josep
Ramon Azcon, Javier
author_role author
author2 Fernández Garibay, Xiomara
Velasco Mallorquí, Ferran
García Castaño, F. Javier
Samitier i Martí, Josep
Ramon Azcon, Javier
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Materials biomèdics
Impressió 3D
Teixits (Histologia)
Biomedical materials
Three-dimensional printing
Tissues
topic Materials biomèdics
Impressió 3D
Teixits (Histologia)
Biomedical materials
Three-dimensional printing
Tissues
description New biocompatible materials have enabled the direct 3D printing of complex functional living tissues, such as skeletal and cardiac muscle. Gelatinmethacryloyl (GelMA) is a photopolymerizable hydrogel composed of natural gelatin functionalized with methacrylic anhydride. However, it is difficult to obtain a single hydrogel that meets all the desirable properties for tissue engineering. In particular, GelMA hydrogels lack versatility in their mechanical properties and lasting 3D structures. In this work, a library of composite biomaterials to obtain versatile, lasting, and mechanically tunable scaffolds are presented. Two polysaccharides, alginate and carboxymethyl cellulose chemically functionalized with methacrylic anhydride, and a synthetic material, such as poly(ethylene glycol) diacrylate are combined with GelMA to obtain photopolymerizable hydrogel blends. Physical properties of the obtained composite hydrogels are screened and optimized for the growth and development of skeletal muscle fibers from C2C12 murine cells, and compared with pristine GelMA. All these composites show high resistance to degradation maintaining the 3D structure with high fidelity over several weeks. Altogether, in this study a library of biocompatible novel and totally versatile composite biomaterials are developed and characterized, with tunable mechanical properties that give structure and support myotube formation and alignment.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/125984
url https://hdl.handle.net/2445/125984
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: http://dx.doi.org/10.1002/mabi.201800167
Macromolecular Bioscience, 2018, vol. 18, num. 10, p. 1800167
https://doi.org/10.1002/mabi.201800167
info:eu-repo/grantAgreement/EC/H2020/714317
dc.rights.none.fl_str_mv (c) Wiley-VCH, 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Wiley-VCH, 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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