IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper

Currently, testing for immunoglobulin E (IgE) sensitization is the cornerstone of diagnostic evaluation in suspected allergic conditions. This review provides a thorough and updated critical appraisal of the most frequently used diagnostic tests, both in vivo and in vitro. It discusses skin tests, c...

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Autores: Ansotegui Zubeldia, Ignacio Javier|||0000-0002-6942-1511, Melioli, Giovanni, Canonica, G. Walter|||0000-0001-8467-2557, Caraballo, Luis, Villa, Elisa, Ebisawa, Motohiro|||0000-0003-4117-558X, Passalacqua, Giovanni|||0000-0002-5139-3604, Savi, Eleonora, Ebo, Didier|||0000-0003-0672-7529, Gómez, R. Maximiliano, Luengo Sánchez, Olga|||0000-0002-8905-2776, Oppenheimer, John J., Jensen-Jarolim, Erika, Fischer, David A., Haahtela, Tari|||0000-0003-4757-2156, Antila, Martti, Bousquet, Jean|||0000-0002-4061-4766, Cardona, Victoria|||0000-0003-2197-9767, Chiang, Wen Chin, Demoly, Pascal|||0000-0001-7827-7964, DuBuske, Lawrence M., Ferrer Puga, Marta, Gerth van Wijk, Roy, González Díaz, Sandra Nora, Gonzalez-Estrada, Alexei, Jares, E. J.|||0000-0001-9411-0582, Kalpaklioğlu, Ayse Füsun, Kase Tanno, Luciana, Kowalski, Marek L., Ledford, Dennis K., Monge Ortega, Olga Patricia, Morais Almeida, Mário, Pfaar, Oliver|||0000-0003-4374-9639, Poulsen, Lars K., Pawankar, Ruby, Renz, Harald E., Romano, Antonino G., Rosário Filho, Nelson A., Rosenwasser, Lanny, Sánchez Borges, Mario|||0000-0002-9308-6418, Scala, Enrico|||0000-0002-9391-9168, Senna, Gian-Enrico, Sisul, Juan Carlos, Tang, Mimi L. K., Thong, Bernard Yu-Hor, Valenta, Rudolf, Wood, Robert A., Zuberbier, Torsten|||0000-0002-1466-8875
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:227656
Acceso en línea:https://ddd.uab.cat/record/227656
https://dx.doi.org/urn:doi:10.1016/j.waojou.2019.100080
Access Level:acceso abierto
Palabra clave:IgE
Allergy
In vitro tests
Skin tests
Diagnostic strategies
AAAAI, American Academy of Allergy Asthma and Immunology
ABA, Allergen Bead Array
ACAAI, American College of Allergy Asthma and Immunology
AIT, allergen immunotherapy
AEC, Allergen Exposure Chambers
Anti-IgE, Antibody against IgE
AP, Alkaline Phosphatase
AU/mL, Allergenic Units milliLiter
BAT, Basophil Activation Test
BAU/mL, Biologic Allergenic Units milliLiter
CaFE, Calibrated Fluorescence Enhancement
CBA, Cytometric Bead Array
CCD, Cross-reactive Carbohydrate Determinants
CDER, Center for Drug Evaluation and Research (USA)
CL, Chemiluminescence
DBPCFC, Double-Blind Placebo-Controlled Food Challenge
EAACI, European Academy of Allergy and Immunology
EIA, Enzyme Immune Assay
ELISA, Enzyme Linked Immuno Sorbent Analysis
EMEA, European MEdicine Agencies
ENPP-3, EctoNucleotide Pyrophosphatase/Phosphodiesterase 3
FACS, Fluorescence-Activated Cell Sorting
FDA, Food and Drug Administration (U.S. Department of Health and Human Services)
FEIA, Fluorescent Enzyme Immunoassays
FcεRI, High affinity IgE receptor
H1, Histamine 1 receptor
H2, Histamine 2 receptor
HPO, Horseradish Peroxidase
IDT, Intradermal Test
IgE, immunoglobulin E
ISAC, Immuno-Solid phase Allergen Chip
IUIS, International Union of Immunological Societies
IVD, in vitro diagnostic tool
Ku/l, kilo Units of Allergen/Liter for allergen-specific IgE antibody assays
LAMP-3, Lysosomal-Associated Membrane Protein
Mab, Monoclonal Antibody
MRGPRX2, Mas-related G protein receptor 2
MBAD, Molecule Based Allergy Diagnostics
NIH, National Institutes of Health (USA)
NMBAs, NeuroMuscular Blocking Agents
NPA, Negative Percent Agreement
NSAIDs, Non-Steroidal Anti-Inflammatory Drugs
Pnpp, p -Nitrophenylphosphate
PPA, Positive Percent Agreement
PPT, Prick-Prick Test
Sige, specific IgE
RAST, Radio Allergo Sorbent Test
SCAR, severe cutaneous adverse drug reactions
SPT, Skin prick test
W/v, weight /volume
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dc.title.none.fl_str_mv IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
title IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
spellingShingle IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
Ansotegui Zubeldia, Ignacio Javier|||0000-0002-6942-1511
IgE
Allergy
In vitro tests
Skin tests
Diagnostic strategies
AAAAI, American Academy of Allergy Asthma and Immunology
ABA, Allergen Bead Array
ACAAI, American College of Allergy Asthma and Immunology
AIT, allergen immunotherapy
AEC, Allergen Exposure Chambers
Anti-IgE, Antibody against IgE
AP, Alkaline Phosphatase
AU/mL, Allergenic Units milliLiter
BAT, Basophil Activation Test
BAU/mL, Biologic Allergenic Units milliLiter
CaFE, Calibrated Fluorescence Enhancement
CBA, Cytometric Bead Array
CCD, Cross-reactive Carbohydrate Determinants
CDER, Center for Drug Evaluation and Research (USA)
CL, Chemiluminescence
DBPCFC, Double-Blind Placebo-Controlled Food Challenge
EAACI, European Academy of Allergy and Immunology
EIA, Enzyme Immune Assay
ELISA, Enzyme Linked Immuno Sorbent Analysis
EMEA, European MEdicine Agencies
ENPP-3, EctoNucleotide Pyrophosphatase/Phosphodiesterase 3
FACS, Fluorescence-Activated Cell Sorting
FDA, Food and Drug Administration (U.S. Department of Health and Human Services)
FEIA, Fluorescent Enzyme Immunoassays
FcεRI, High affinity IgE receptor
H1, Histamine 1 receptor
H2, Histamine 2 receptor
HPO, Horseradish Peroxidase
IDT, Intradermal Test
IgE, immunoglobulin E
ISAC, Immuno-Solid phase Allergen Chip
IUIS, International Union of Immunological Societies
IVD, in vitro diagnostic tool
Ku/l, kilo Units of Allergen/Liter for allergen-specific IgE antibody assays
LAMP-3, Lysosomal-Associated Membrane Protein
Mab, Monoclonal Antibody
MRGPRX2, Mas-related G protein receptor 2
MBAD, Molecule Based Allergy Diagnostics
NIH, National Institutes of Health (USA)
NMBAs, NeuroMuscular Blocking Agents
NPA, Negative Percent Agreement
NSAIDs, Non-Steroidal Anti-Inflammatory Drugs
Pnpp, p -Nitrophenylphosphate
PPA, Positive Percent Agreement
PPT, Prick-Prick Test
Sige, specific IgE
RAST, Radio Allergo Sorbent Test
SCAR, severe cutaneous adverse drug reactions
SPT, Skin prick test
W/v, weight /volume
title_short IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
title_full IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
title_fullStr IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
title_full_unstemmed IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
title_sort IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paper
dc.creator.none.fl_str_mv Ansotegui Zubeldia, Ignacio Javier|||0000-0002-6942-1511
Melioli, Giovanni
Canonica, G. Walter|||0000-0001-8467-2557
Caraballo, Luis
Villa, Elisa
Ebisawa, Motohiro|||0000-0003-4117-558X
Passalacqua, Giovanni|||0000-0002-5139-3604
Savi, Eleonora
Ebo, Didier|||0000-0003-0672-7529
Gómez, R. Maximiliano
Luengo Sánchez, Olga|||0000-0002-8905-2776
Oppenheimer, John J.
Jensen-Jarolim, Erika
Fischer, David A.
Haahtela, Tari|||0000-0003-4757-2156
Antila, Martti
Bousquet, Jean|||0000-0002-4061-4766
Cardona, Victoria|||0000-0003-2197-9767
Chiang, Wen Chin
Demoly, Pascal|||0000-0001-7827-7964
DuBuske, Lawrence M.
Ferrer Puga, Marta
Gerth van Wijk, Roy
González Díaz, Sandra Nora
Gonzalez-Estrada, Alexei
Jares, E. J.|||0000-0001-9411-0582
Kalpaklioğlu, Ayse Füsun
Kase Tanno, Luciana
Kowalski, Marek L.
Ledford, Dennis K.
Monge Ortega, Olga Patricia
Morais Almeida, Mário
Pfaar, Oliver|||0000-0003-4374-9639
Poulsen, Lars K.
Pawankar, Ruby
Renz, Harald E.
Romano, Antonino G.
Rosário Filho, Nelson A.
Rosenwasser, Lanny
Sánchez Borges, Mario|||0000-0002-9308-6418
Scala, Enrico|||0000-0002-9391-9168
Senna, Gian-Enrico
Sisul, Juan Carlos
Tang, Mimi L. K.
Thong, Bernard Yu-Hor
Valenta, Rudolf
Wood, Robert A.
Zuberbier, Torsten|||0000-0002-1466-8875
author Ansotegui Zubeldia, Ignacio Javier|||0000-0002-6942-1511
author_facet Ansotegui Zubeldia, Ignacio Javier|||0000-0002-6942-1511
Melioli, Giovanni
Canonica, G. Walter|||0000-0001-8467-2557
Caraballo, Luis
Villa, Elisa
Ebisawa, Motohiro|||0000-0003-4117-558X
Passalacqua, Giovanni|||0000-0002-5139-3604
Savi, Eleonora
Ebo, Didier|||0000-0003-0672-7529
Gómez, R. Maximiliano
Luengo Sánchez, Olga|||0000-0002-8905-2776
Oppenheimer, John J.
Jensen-Jarolim, Erika
Fischer, David A.
Haahtela, Tari|||0000-0003-4757-2156
Antila, Martti
Bousquet, Jean|||0000-0002-4061-4766
Cardona, Victoria|||0000-0003-2197-9767
Chiang, Wen Chin
Demoly, Pascal|||0000-0001-7827-7964
DuBuske, Lawrence M.
Ferrer Puga, Marta
Gerth van Wijk, Roy
González Díaz, Sandra Nora
Gonzalez-Estrada, Alexei
Jares, E. J.|||0000-0001-9411-0582
Kalpaklioğlu, Ayse Füsun
Kase Tanno, Luciana
Kowalski, Marek L.
Ledford, Dennis K.
Monge Ortega, Olga Patricia
Morais Almeida, Mário
Pfaar, Oliver|||0000-0003-4374-9639
Poulsen, Lars K.
Pawankar, Ruby
Renz, Harald E.
Romano, Antonino G.
Rosário Filho, Nelson A.
Rosenwasser, Lanny
Sánchez Borges, Mario|||0000-0002-9308-6418
Scala, Enrico|||0000-0002-9391-9168
Senna, Gian-Enrico
Sisul, Juan Carlos
Tang, Mimi L. K.
Thong, Bernard Yu-Hor
Valenta, Rudolf
Wood, Robert A.
Zuberbier, Torsten|||0000-0002-1466-8875
author_role author
author2 Melioli, Giovanni
Canonica, G. Walter|||0000-0001-8467-2557
Caraballo, Luis
Villa, Elisa
Ebisawa, Motohiro|||0000-0003-4117-558X
Passalacqua, Giovanni|||0000-0002-5139-3604
Savi, Eleonora
Ebo, Didier|||0000-0003-0672-7529
Gómez, R. Maximiliano
Luengo Sánchez, Olga|||0000-0002-8905-2776
Oppenheimer, John J.
Jensen-Jarolim, Erika
Fischer, David A.
Haahtela, Tari|||0000-0003-4757-2156
Antila, Martti
Bousquet, Jean|||0000-0002-4061-4766
Cardona, Victoria|||0000-0003-2197-9767
Chiang, Wen Chin
Demoly, Pascal|||0000-0001-7827-7964
DuBuske, Lawrence M.
Ferrer Puga, Marta
Gerth van Wijk, Roy
González Díaz, Sandra Nora
Gonzalez-Estrada, Alexei
Jares, E. J.|||0000-0001-9411-0582
Kalpaklioğlu, Ayse Füsun
Kase Tanno, Luciana
Kowalski, Marek L.
Ledford, Dennis K.
Monge Ortega, Olga Patricia
Morais Almeida, Mário
Pfaar, Oliver|||0000-0003-4374-9639
Poulsen, Lars K.
Pawankar, Ruby
Renz, Harald E.
Romano, Antonino G.
Rosário Filho, Nelson A.
Rosenwasser, Lanny
Sánchez Borges, Mario|||0000-0002-9308-6418
Scala, Enrico|||0000-0002-9391-9168
Senna, Gian-Enrico
Sisul, Juan Carlos
Tang, Mimi L. K.
Thong, Bernard Yu-Hor
Valenta, Rudolf
Wood, Robert A.
Zuberbier, Torsten|||0000-0002-1466-8875
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
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author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv IgE
Allergy
In vitro tests
Skin tests
Diagnostic strategies
AAAAI, American Academy of Allergy Asthma and Immunology
ABA, Allergen Bead Array
ACAAI, American College of Allergy Asthma and Immunology
AIT, allergen immunotherapy
AEC, Allergen Exposure Chambers
Anti-IgE, Antibody against IgE
AP, Alkaline Phosphatase
AU/mL, Allergenic Units milliLiter
BAT, Basophil Activation Test
BAU/mL, Biologic Allergenic Units milliLiter
CaFE, Calibrated Fluorescence Enhancement
CBA, Cytometric Bead Array
CCD, Cross-reactive Carbohydrate Determinants
CDER, Center for Drug Evaluation and Research (USA)
CL, Chemiluminescence
DBPCFC, Double-Blind Placebo-Controlled Food Challenge
EAACI, European Academy of Allergy and Immunology
EIA, Enzyme Immune Assay
ELISA, Enzyme Linked Immuno Sorbent Analysis
EMEA, European MEdicine Agencies
ENPP-3, EctoNucleotide Pyrophosphatase/Phosphodiesterase 3
FACS, Fluorescence-Activated Cell Sorting
FDA, Food and Drug Administration (U.S. Department of Health and Human Services)
FEIA, Fluorescent Enzyme Immunoassays
FcεRI, High affinity IgE receptor
H1, Histamine 1 receptor
H2, Histamine 2 receptor
HPO, Horseradish Peroxidase
IDT, Intradermal Test
IgE, immunoglobulin E
ISAC, Immuno-Solid phase Allergen Chip
IUIS, International Union of Immunological Societies
IVD, in vitro diagnostic tool
Ku/l, kilo Units of Allergen/Liter for allergen-specific IgE antibody assays
LAMP-3, Lysosomal-Associated Membrane Protein
Mab, Monoclonal Antibody
MRGPRX2, Mas-related G protein receptor 2
MBAD, Molecule Based Allergy Diagnostics
NIH, National Institutes of Health (USA)
NMBAs, NeuroMuscular Blocking Agents
NPA, Negative Percent Agreement
NSAIDs, Non-Steroidal Anti-Inflammatory Drugs
Pnpp, p -Nitrophenylphosphate
PPA, Positive Percent Agreement
PPT, Prick-Prick Test
Sige, specific IgE
RAST, Radio Allergo Sorbent Test
SCAR, severe cutaneous adverse drug reactions
SPT, Skin prick test
W/v, weight /volume
topic IgE
Allergy
In vitro tests
Skin tests
Diagnostic strategies
AAAAI, American Academy of Allergy Asthma and Immunology
ABA, Allergen Bead Array
ACAAI, American College of Allergy Asthma and Immunology
AIT, allergen immunotherapy
AEC, Allergen Exposure Chambers
Anti-IgE, Antibody against IgE
AP, Alkaline Phosphatase
AU/mL, Allergenic Units milliLiter
BAT, Basophil Activation Test
BAU/mL, Biologic Allergenic Units milliLiter
CaFE, Calibrated Fluorescence Enhancement
CBA, Cytometric Bead Array
CCD, Cross-reactive Carbohydrate Determinants
CDER, Center for Drug Evaluation and Research (USA)
CL, Chemiluminescence
DBPCFC, Double-Blind Placebo-Controlled Food Challenge
EAACI, European Academy of Allergy and Immunology
EIA, Enzyme Immune Assay
ELISA, Enzyme Linked Immuno Sorbent Analysis
EMEA, European MEdicine Agencies
ENPP-3, EctoNucleotide Pyrophosphatase/Phosphodiesterase 3
FACS, Fluorescence-Activated Cell Sorting
FDA, Food and Drug Administration (U.S. Department of Health and Human Services)
FEIA, Fluorescent Enzyme Immunoassays
FcεRI, High affinity IgE receptor
H1, Histamine 1 receptor
H2, Histamine 2 receptor
HPO, Horseradish Peroxidase
IDT, Intradermal Test
IgE, immunoglobulin E
ISAC, Immuno-Solid phase Allergen Chip
IUIS, International Union of Immunological Societies
IVD, in vitro diagnostic tool
Ku/l, kilo Units of Allergen/Liter for allergen-specific IgE antibody assays
LAMP-3, Lysosomal-Associated Membrane Protein
Mab, Monoclonal Antibody
MRGPRX2, Mas-related G protein receptor 2
MBAD, Molecule Based Allergy Diagnostics
NIH, National Institutes of Health (USA)
NMBAs, NeuroMuscular Blocking Agents
NPA, Negative Percent Agreement
NSAIDs, Non-Steroidal Anti-Inflammatory Drugs
Pnpp, p -Nitrophenylphosphate
PPA, Positive Percent Agreement
PPT, Prick-Prick Test
Sige, specific IgE
RAST, Radio Allergo Sorbent Test
SCAR, severe cutaneous adverse drug reactions
SPT, Skin prick test
W/v, weight /volume
description Currently, testing for immunoglobulin E (IgE) sensitization is the cornerstone of diagnostic evaluation in suspected allergic conditions. This review provides a thorough and updated critical appraisal of the most frequently used diagnostic tests, both in vivo and in vitro. It discusses skin tests, challenges, and serological and cellular in vitro tests, and provides an overview of indications, advantages and disadvantages of each in conditions such as respiratory, food, venom, drug, and occupational allergy. Skin prick testing remains the first line approach in most instances; the added value of serum specific IgE to whole allergen extracts or components, as well as the role of basophil activation tests, is evaluated. Unproven, non-validated, diagnostic tests are also discussed. Throughout the review, the reader must bear in mind the relevance of differentiating between sensitization and allergy; the latter entails not only allergic sensitization, but also clinically relevant symptoms triggered by the culprit allergen.
publishDate 2020
dc.date.none.fl_str_mv 2
2020-01-01
2020
2020-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/227656
https://dx.doi.org/urn:doi:10.1016/j.waojou.2019.100080
url https://ddd.uab.cat/record/227656
https://dx.doi.org/urn:doi:10.1016/j.waojou.2019.100080
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling IgE allergy diagnostics and other relevant tests in allergy, a World Allergy Organization position paperAnsotegui Zubeldia, Ignacio Javier|||0000-0002-6942-1511Melioli, GiovanniCanonica, G. Walter|||0000-0001-8467-2557Caraballo, LuisVilla, ElisaEbisawa, Motohiro|||0000-0003-4117-558XPassalacqua, Giovanni|||0000-0002-5139-3604Savi, EleonoraEbo, Didier|||0000-0003-0672-7529Gómez, R. MaximilianoLuengo Sánchez, Olga|||0000-0002-8905-2776Oppenheimer, John J.Jensen-Jarolim, ErikaFischer, David A.Haahtela, Tari|||0000-0003-4757-2156Antila, MarttiBousquet, Jean|||0000-0002-4061-4766Cardona, Victoria|||0000-0003-2197-9767Chiang, Wen ChinDemoly, Pascal|||0000-0001-7827-7964DuBuske, Lawrence M.Ferrer Puga, MartaGerth van Wijk, RoyGonzález Díaz, Sandra NoraGonzalez-Estrada, AlexeiJares, E. J.|||0000-0001-9411-0582Kalpaklioğlu, Ayse FüsunKase Tanno, LucianaKowalski, Marek L.Ledford, Dennis K.Monge Ortega, Olga PatriciaMorais Almeida, MárioPfaar, Oliver|||0000-0003-4374-9639Poulsen, Lars K.Pawankar, RubyRenz, Harald E.Romano, Antonino G.Rosário Filho, Nelson A.Rosenwasser, LannySánchez Borges, Mario|||0000-0002-9308-6418Scala, Enrico|||0000-0002-9391-9168Senna, Gian-EnricoSisul, Juan CarlosTang, Mimi L. K.Thong, Bernard Yu-HorValenta, RudolfWood, Robert A.Zuberbier, Torsten|||0000-0002-1466-8875IgEAllergyIn vitro testsSkin testsDiagnostic strategiesAAAAI, American Academy of Allergy Asthma and ImmunologyABA, Allergen Bead ArrayACAAI, American College of Allergy Asthma and ImmunologyAIT, allergen immunotherapyAEC, Allergen Exposure ChambersAnti-IgE, Antibody against IgEAP, Alkaline PhosphataseAU/mL, Allergenic Units milliLiterBAT, Basophil Activation TestBAU/mL, Biologic Allergenic Units milliLiterCaFE, Calibrated Fluorescence EnhancementCBA, Cytometric Bead ArrayCCD, Cross-reactive Carbohydrate DeterminantsCDER, Center for Drug Evaluation and Research (USA)CL, ChemiluminescenceDBPCFC, Double-Blind Placebo-Controlled Food ChallengeEAACI, European Academy of Allergy and ImmunologyEIA, Enzyme Immune AssayELISA, Enzyme Linked Immuno Sorbent AnalysisEMEA, European MEdicine AgenciesENPP-3, EctoNucleotide Pyrophosphatase/Phosphodiesterase 3FACS, Fluorescence-Activated Cell SortingFDA, Food and Drug Administration (U.S. Department of Health and Human Services)FEIA, Fluorescent Enzyme ImmunoassaysFcεRI, High affinity IgE receptorH1, Histamine 1 receptorH2, Histamine 2 receptorHPO, Horseradish PeroxidaseIDT, Intradermal TestIgE, immunoglobulin EISAC, Immuno-Solid phase Allergen ChipIUIS, International Union of Immunological SocietiesIVD, in vitro diagnostic toolKu/l, kilo Units of Allergen/Liter for allergen-specific IgE antibody assaysLAMP-3, Lysosomal-Associated Membrane ProteinMab, Monoclonal AntibodyMRGPRX2, Mas-related G protein receptor 2MBAD, Molecule Based Allergy DiagnosticsNIH, National Institutes of Health (USA)NMBAs, NeuroMuscular Blocking AgentsNPA, Negative Percent AgreementNSAIDs, Non-Steroidal Anti-Inflammatory DrugsPnpp, p -NitrophenylphosphatePPA, Positive Percent AgreementPPT, Prick-Prick TestSige, specific IgERAST, Radio Allergo Sorbent TestSCAR, severe cutaneous adverse drug reactionsSPT, Skin prick testW/v, weight /volumeCurrently, testing for immunoglobulin E (IgE) sensitization is the cornerstone of diagnostic evaluation in suspected allergic conditions. This review provides a thorough and updated critical appraisal of the most frequently used diagnostic tests, both in vivo and in vitro. It discusses skin tests, challenges, and serological and cellular in vitro tests, and provides an overview of indications, advantages and disadvantages of each in conditions such as respiratory, food, venom, drug, and occupational allergy. Skin prick testing remains the first line approach in most instances; the added value of serum specific IgE to whole allergen extracts or components, as well as the role of basophil activation tests, is evaluated. Unproven, non-validated, diagnostic tests are also discussed. Throughout the review, the reader must bear in mind the relevance of differentiating between sensitization and allergy; the latter entails not only allergic sensitization, but also clinically relevant symptoms triggered by the culprit allergen.Universitat Autònoma de Barcelona 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/227656https://dx.doi.org/urn:doi:10.1016/j.waojou.2019.100080reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2276562026-06-06T12:50:31Z
score 15.300724