Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium

The current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b.w.) were...

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Autores: Rodríguez-Vázquez, Lucía, Martí-Clúa, Joaquín|||0000-0002-6774-0092
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:288677
Acceso en línea:https://ddd.uab.cat/record/288677
https://dx.doi.org/urn:doi:10.1002/cne.25005
Access Level:acceso abierto
Palabra clave:5-bromo-2'-deoxyuridine
RRID: AB_10013660
RRID: AB_2313609
RRID: AB_258588
RRID: AB_476884
RRID: AB_628110
Apoptosis
Cerebellar neuroepithelium
Embryonic life
Immunohistochemistry
Transmission electron microscopy
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spelling Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepitheliumRodríguez-Vázquez, LucíaMartí-Clúa, Joaquín|||0000-0002-6774-00925-bromo-2'-deoxyuridineRRID: AB_10013660RRID: AB_2313609RRID: AB_258588RRID: AB_476884RRID: AB_628110ApoptosisCerebellar neuroepitheliumEmbryonic lifeImmunohistochemistryTransmission electron microscopyThe current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b.w.) were analyzed using immunohistochemical and ultrastructural procedures. The pregnant rats were injected with BrdU at embryonic day 13, and their fetuses were sacrificed from 5 to 35 hr after exposure. The quantification of several parameters such as the density of mitotic figures, and BrdU and proliferating cell nuclear antigen (PCNA)-reactive cells showed that, in comparison with the saline injected rats, the administration of BrdU impairs the proliferative behavior of neuroepithelial cells. The above-mentioned parameters were significantly reduced in rats injected with 100 mu g/g b.w. of BrdU. The reduction was more evident using 200 mu g/g b.w. The most severe effects were found with 300 mu g/g b.w. of BrdU. The present findings also revealed that high doses of BrdU lead to the activation of apoptotic cellular events as evidenced by both terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and immunohistochemistry for active caspase-3. In comparison with saline rats, many apoptotic cells were found in rats injected with 100 mu g/g b.w. of BrdU. The number of dying cells increased with 200 mu g/g b.w. The most important number of apoptotic cells were observed in animals injected with 300 mu g/g b.w. of BrdU. Ultrastructural studies confirmed the presence of neuroblasts at different stages of apoptosis. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/288677https://dx.doi.org/urn:doi:10.1002/cne.25005reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.https://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2886772026-06-06T12:50:31Z
dc.title.none.fl_str_mv Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
title Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
spellingShingle Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
Rodríguez-Vázquez, Lucía
5-bromo-2'-deoxyuridine
RRID: AB_10013660
RRID: AB_2313609
RRID: AB_258588
RRID: AB_476884
RRID: AB_628110
Apoptosis
Cerebellar neuroepithelium
Embryonic life
Immunohistochemistry
Transmission electron microscopy
title_short Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
title_full Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
title_fullStr Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
title_full_unstemmed Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
title_sort Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
dc.creator.none.fl_str_mv Rodríguez-Vázquez, Lucía
Martí-Clúa, Joaquín|||0000-0002-6774-0092
author Rodríguez-Vázquez, Lucía
author_facet Rodríguez-Vázquez, Lucía
Martí-Clúa, Joaquín|||0000-0002-6774-0092
author_role author
author2 Martí-Clúa, Joaquín|||0000-0002-6774-0092
author2_role author
dc.subject.none.fl_str_mv 5-bromo-2'-deoxyuridine
RRID: AB_10013660
RRID: AB_2313609
RRID: AB_258588
RRID: AB_476884
RRID: AB_628110
Apoptosis
Cerebellar neuroepithelium
Embryonic life
Immunohistochemistry
Transmission electron microscopy
topic 5-bromo-2'-deoxyuridine
RRID: AB_10013660
RRID: AB_2313609
RRID: AB_258588
RRID: AB_476884
RRID: AB_628110
Apoptosis
Cerebellar neuroepithelium
Embryonic life
Immunohistochemistry
Transmission electron microscopy
description The current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b.w.) were analyzed using immunohistochemical and ultrastructural procedures. The pregnant rats were injected with BrdU at embryonic day 13, and their fetuses were sacrificed from 5 to 35 hr after exposure. The quantification of several parameters such as the density of mitotic figures, and BrdU and proliferating cell nuclear antigen (PCNA)-reactive cells showed that, in comparison with the saline injected rats, the administration of BrdU impairs the proliferative behavior of neuroepithelial cells. The above-mentioned parameters were significantly reduced in rats injected with 100 mu g/g b.w. of BrdU. The reduction was more evident using 200 mu g/g b.w. The most severe effects were found with 300 mu g/g b.w. of BrdU. The present findings also revealed that high doses of BrdU lead to the activation of apoptotic cellular events as evidenced by both terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and immunohistochemistry for active caspase-3. In comparison with saline rats, many apoptotic cells were found in rats injected with 100 mu g/g b.w. of BrdU. The number of dying cells increased with 200 mu g/g b.w. The most important number of apoptotic cells were observed in animals injected with 300 mu g/g b.w. of BrdU. Ultrastructural studies confirmed the presence of neuroblasts at different stages of apoptosis.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/288677
https://dx.doi.org/urn:doi:10.1002/cne.25005
url https://ddd.uab.cat/record/288677
https://dx.doi.org/urn:doi:10.1002/cne.25005
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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