Administration of 5-bromo-2'-deoxyuridine interferes with neuroblast proliferation and promotes apoptotic cell death in the rat cerebellar neuroepithelium
The current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b.w.) were...
| Autores: | , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:288677 |
| Acceso en línea: | https://ddd.uab.cat/record/288677 https://dx.doi.org/urn:doi:10.1002/cne.25005 |
| Access Level: | acceso abierto |
| Palabra clave: | 5-bromo-2'-deoxyuridine RRID: AB_10013660 RRID: AB_2313609 RRID: AB_258588 RRID: AB_476884 RRID: AB_628110 Apoptosis Cerebellar neuroepithelium Embryonic life Immunohistochemistry Transmission electron microscopy |
| Sumario: | The current study was conducted to assess whether a single administration of 5-bromo-2'-deoxyuridine (BrdU) interferes with cell proliferation and leads to the activation of apoptotic cellular events in the prenatal cerebellum. BrdU effects across a wide range of doses (25-300 mu g/g b.w.) were analyzed using immunohistochemical and ultrastructural procedures. The pregnant rats were injected with BrdU at embryonic day 13, and their fetuses were sacrificed from 5 to 35 hr after exposure. The quantification of several parameters such as the density of mitotic figures, and BrdU and proliferating cell nuclear antigen (PCNA)-reactive cells showed that, in comparison with the saline injected rats, the administration of BrdU impairs the proliferative behavior of neuroepithelial cells. The above-mentioned parameters were significantly reduced in rats injected with 100 mu g/g b.w. of BrdU. The reduction was more evident using 200 mu g/g b.w. The most severe effects were found with 300 mu g/g b.w. of BrdU. The present findings also revealed that high doses of BrdU lead to the activation of apoptotic cellular events as evidenced by both terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay and immunohistochemistry for active caspase-3. In comparison with saline rats, many apoptotic cells were found in rats injected with 100 mu g/g b.w. of BrdU. The number of dying cells increased with 200 mu g/g b.w. The most important number of apoptotic cells were observed in animals injected with 300 mu g/g b.w. of BrdU. Ultrastructural studies confirmed the presence of neuroblasts at different stages of apoptosis. |
|---|