Yeast as a Heterologous Model System to Uncover Type III Effector Function

Type III effectors (T3E) are key virulence proteins that are injected by bacterial pathogens inside the cells of their host to subvert cellular processes and contribute to disease. The budding yeast Saccharomyces cerevisiae represents an important heterologous system for the functional characterisat...

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Autores: Popa, Crina Mihaela, Coll, Núria S., Valls i Matheu, Marc, Sessa, G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/103383
Acceso en línea:https://hdl.handle.net/2445/103383
Access Level:acceso abierto
Palabra clave:Citoquines
Genètica molecular
Saccharomyces cerevisiae
Cytokines
Molecular genetics
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spelling Yeast as a Heterologous Model System to Uncover Type III Effector FunctionPopa, Crina MihaelaColl, Núria S.Valls i Matheu, MarcSessa, G.CitoquinesGenètica molecularSaccharomyces cerevisiaeCytokinesMolecular geneticsSaccharomyces cerevisiaeType III effectors (T3E) are key virulence proteins that are injected by bacterial pathogens inside the cells of their host to subvert cellular processes and contribute to disease. The budding yeast Saccharomyces cerevisiae represents an important heterologous system for the functional characterisation of T3E proteins in a eukaryotic environment. Importantly, yeast contains eukaryotic processes with low redundancy and are devoid of immunity mechanisms that counteract T3Es and mask their function. Expression in yeast of effectors from both plant and animal pathogens that perturb conserved cellular processes often resulted in robust phenotypes that were exploited to elucidate effector functions, biochemical properties, and host targets. The genetic tractability of yeast and its amenability for highthroughput functional studies contributed to the success of this system that, in recent years, has been used to study over 100 effectors. Here, we provide a critical view on this body of work and describe advantages and limitations inherent to the use of yeast in T3E research. 'Favourite' targets of T3Es in yeast are cytoskeleton components and small GTPases of the Rho family.We describe how mitogen-activated protein kinase (MAPK) signalling, vesicle trafficking, membrane structures, and programmed cell death are also often altered by T3Es in yeast and how this reflects their function in the natural host. We describe how effector structure-function studies and analysis of candidate targeted processes or pathways can be carried out in yeast. We critically analyse technologies that have been used in yeast to assign biochemical functions to T3Es, including transcriptomics and proteomics, as well as suppressor, gain-of-function, or synthetic lethality screens. We also describe how yeast can be used to select for molecules that block T3E function in search of new antibacterial drugs with medical applications. Finally, we provide our opinion on the limitations of S. cerevisiae as a model system and its most promising future applications.Public Library of Science (PLoS)2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/103383Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1371/journal.ppat.1005360PLoS Pathogens, 2016, vol. 12, num. 2, p. 1-17https://doi.org/10.1371/journal.ppat.1005360info:eu-repo/grantAgreement/EC/FP7/321738info:eu-repo/grantAgreement/EC/FP7/331392cc-by (c) Popa, C. et al., 2016http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1033832026-05-27T06:46:51Z
dc.title.none.fl_str_mv Yeast as a Heterologous Model System to Uncover Type III Effector Function
title Yeast as a Heterologous Model System to Uncover Type III Effector Function
spellingShingle Yeast as a Heterologous Model System to Uncover Type III Effector Function
Popa, Crina Mihaela
Citoquines
Genètica molecular
Saccharomyces cerevisiae
Cytokines
Molecular genetics
Saccharomyces cerevisiae
title_short Yeast as a Heterologous Model System to Uncover Type III Effector Function
title_full Yeast as a Heterologous Model System to Uncover Type III Effector Function
title_fullStr Yeast as a Heterologous Model System to Uncover Type III Effector Function
title_full_unstemmed Yeast as a Heterologous Model System to Uncover Type III Effector Function
title_sort Yeast as a Heterologous Model System to Uncover Type III Effector Function
dc.creator.none.fl_str_mv Popa, Crina Mihaela
Coll, Núria S.
Valls i Matheu, Marc
Sessa, G.
author Popa, Crina Mihaela
author_facet Popa, Crina Mihaela
Coll, Núria S.
Valls i Matheu, Marc
Sessa, G.
author_role author
author2 Coll, Núria S.
Valls i Matheu, Marc
Sessa, G.
author2_role author
author
author
dc.subject.none.fl_str_mv Citoquines
Genètica molecular
Saccharomyces cerevisiae
Cytokines
Molecular genetics
Saccharomyces cerevisiae
topic Citoquines
Genètica molecular
Saccharomyces cerevisiae
Cytokines
Molecular genetics
Saccharomyces cerevisiae
description Type III effectors (T3E) are key virulence proteins that are injected by bacterial pathogens inside the cells of their host to subvert cellular processes and contribute to disease. The budding yeast Saccharomyces cerevisiae represents an important heterologous system for the functional characterisation of T3E proteins in a eukaryotic environment. Importantly, yeast contains eukaryotic processes with low redundancy and are devoid of immunity mechanisms that counteract T3Es and mask their function. Expression in yeast of effectors from both plant and animal pathogens that perturb conserved cellular processes often resulted in robust phenotypes that were exploited to elucidate effector functions, biochemical properties, and host targets. The genetic tractability of yeast and its amenability for highthroughput functional studies contributed to the success of this system that, in recent years, has been used to study over 100 effectors. Here, we provide a critical view on this body of work and describe advantages and limitations inherent to the use of yeast in T3E research. 'Favourite' targets of T3Es in yeast are cytoskeleton components and small GTPases of the Rho family.We describe how mitogen-activated protein kinase (MAPK) signalling, vesicle trafficking, membrane structures, and programmed cell death are also often altered by T3Es in yeast and how this reflects their function in the natural host. We describe how effector structure-function studies and analysis of candidate targeted processes or pathways can be carried out in yeast. We critically analyse technologies that have been used in yeast to assign biochemical functions to T3Es, including transcriptomics and proteomics, as well as suppressor, gain-of-function, or synthetic lethality screens. We also describe how yeast can be used to select for molecules that block T3E function in search of new antibacterial drugs with medical applications. Finally, we provide our opinion on the limitations of S. cerevisiae as a model system and its most promising future applications.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/103383
url https://hdl.handle.net/2445/103383
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.ppat.1005360
PLoS Pathogens, 2016, vol. 12, num. 2, p. 1-17
https://doi.org/10.1371/journal.ppat.1005360
info:eu-repo/grantAgreement/EC/FP7/321738
info:eu-repo/grantAgreement/EC/FP7/331392
dc.rights.none.fl_str_mv cc-by (c) Popa, C. et al., 2016
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Popa, C. et al., 2016
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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