Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain

Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for M...

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Autores: Rivaya, Belén|||0000-0003-2562-6248, Le Roy, Chloé|||0000-0003-3235-9264, Jordana-Lluch, Elena, Fernandez Rivas, Gema|||0000-0003-4854-5934, Casañ, Cristina, González Soler, Victoria|||0000-0002-0983-2052, Wang-Wang, Jun Hao|||0000-0003-2609-6453, Bébéar, Cécile|||0000-0001-6065-2332, Matas, Lurdes|||0000-0001-6117-4096, Pereyre, Sabine|||0000-0003-1757-4206
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:302552
Acceso en línea:https://ddd.uab.cat/record/302552
https://dx.doi.org/urn:doi:10.3390/antibiotics11040485
Access Level:acceso abierto
Palabra clave:Mycoplasma genitalium
Macrolide
Fluoroquinolone
Resistance
Spain
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spelling Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, SpainRivaya, Belén|||0000-0003-2562-6248Le Roy, Chloé|||0000-0003-3235-9264Jordana-Lluch, ElenaFernandez Rivas, Gema|||0000-0003-4854-5934Casañ, CristinaGonzález Soler, Victoria|||0000-0002-0983-2052Wang-Wang, Jun Hao|||0000-0003-2609-6453Bébéar, Cécile|||0000-0001-6065-2332Matas, Lurdes|||0000-0001-6117-4096Pereyre, Sabine|||0000-0003-1757-4206Mycoplasma genitaliumMacrolideFluoroquinoloneResistanceSpainMacrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus ® MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the parC gene. Regarding MG detection, Allplex and Resistance Plus ® showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient's management applying resistance-guided treatment strategies. 22022-01-0120222022-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/302552https://dx.doi.org/urn:doi:10.3390/antibiotics11040485reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3025522026-06-06T12:50:31Z
dc.title.none.fl_str_mv Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
title Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
spellingShingle Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
Rivaya, Belén|||0000-0003-2562-6248
Mycoplasma genitalium
Macrolide
Fluoroquinolone
Resistance
Spain
title_short Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
title_full Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
title_fullStr Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
title_full_unstemmed Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
title_sort Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain
dc.creator.none.fl_str_mv Rivaya, Belén|||0000-0003-2562-6248
Le Roy, Chloé|||0000-0003-3235-9264
Jordana-Lluch, Elena
Fernandez Rivas, Gema|||0000-0003-4854-5934
Casañ, Cristina
González Soler, Victoria|||0000-0002-0983-2052
Wang-Wang, Jun Hao|||0000-0003-2609-6453
Bébéar, Cécile|||0000-0001-6065-2332
Matas, Lurdes|||0000-0001-6117-4096
Pereyre, Sabine|||0000-0003-1757-4206
author Rivaya, Belén|||0000-0003-2562-6248
author_facet Rivaya, Belén|||0000-0003-2562-6248
Le Roy, Chloé|||0000-0003-3235-9264
Jordana-Lluch, Elena
Fernandez Rivas, Gema|||0000-0003-4854-5934
Casañ, Cristina
González Soler, Victoria|||0000-0002-0983-2052
Wang-Wang, Jun Hao|||0000-0003-2609-6453
Bébéar, Cécile|||0000-0001-6065-2332
Matas, Lurdes|||0000-0001-6117-4096
Pereyre, Sabine|||0000-0003-1757-4206
author_role author
author2 Le Roy, Chloé|||0000-0003-3235-9264
Jordana-Lluch, Elena
Fernandez Rivas, Gema|||0000-0003-4854-5934
Casañ, Cristina
González Soler, Victoria|||0000-0002-0983-2052
Wang-Wang, Jun Hao|||0000-0003-2609-6453
Bébéar, Cécile|||0000-0001-6065-2332
Matas, Lurdes|||0000-0001-6117-4096
Pereyre, Sabine|||0000-0003-1757-4206
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycoplasma genitalium
Macrolide
Fluoroquinolone
Resistance
Spain
topic Mycoplasma genitalium
Macrolide
Fluoroquinolone
Resistance
Spain
description Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus ® MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the parC gene. Regarding MG detection, Allplex and Resistance Plus ® showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient's management applying resistance-guided treatment strategies.
publishDate 2022
dc.date.none.fl_str_mv 2
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/302552
https://dx.doi.org/urn:doi:10.3390/antibiotics11040485
url https://ddd.uab.cat/record/302552
https://dx.doi.org/urn:doi:10.3390/antibiotics11040485
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
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collection Dipòsit Digital de Documents de la UAB
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