Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain

Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for M...

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Detalles Bibliográficos
Autores: Rivaya, Belén|||0000-0003-2562-6248, Le Roy, Chloé|||0000-0003-3235-9264, Jordana-Lluch, Elena, Fernandez Rivas, Gema|||0000-0003-4854-5934, Casañ, Cristina, González Soler, Victoria|||0000-0002-0983-2052, Wang-Wang, Jun Hao|||0000-0003-2609-6453, Bébéar, Cécile|||0000-0001-6065-2332, Matas, Lurdes|||0000-0001-6117-4096, Pereyre, Sabine|||0000-0003-1757-4206
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:302552
Acceso en línea:https://ddd.uab.cat/record/302552
https://dx.doi.org/urn:doi:10.3390/antibiotics11040485
Access Level:acceso abierto
Palabra clave:Mycoplasma genitalium
Macrolide
Fluoroquinolone
Resistance
Spain
Descripción
Sumario:Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus ® MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the parC gene. Regarding MG detection, Allplex and Resistance Plus ® showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient's management applying resistance-guided treatment strategies.

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