DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp

This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics dem...

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Detalhes bibliográficos
Autores: Reva, O.N., La Cono, V., Marturano, L., Crisafi, F., Smedile, F., Mudaliyar, M., Ghosal, D., Selivanova, E.A., Ignatenko, M.E., Ferrer, Miguel, Fernandez-Lopez, L., Krupovic, M., Yakimov, M.M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/425666
Acesso em linha:http://hdl.handle.net/10261/425666
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85215853352&doi=10.1016%2Fj.isci.2025.111749&partnerID=40&md5=9c7777073c1daea0db115d97b9b59f8d
Access Level:acceso abierto
Palavra-chave:Biochemistry
Environmental Biotechnology
Microbiology
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spelling DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus spReva, O.N.La Cono, V.Marturano, L.Crisafi, F.Smedile, F.Mudaliyar, M.Ghosal, D.Selivanova, E.A.Ignatenko, M.E.Ferrer, MiguelFernandez-Lopez, L.Krupovic, M.Yakimov, M.M.BiochemistryEnvironmental BiotechnologyMicrobiologyThis study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics demonstrated that the tripartite consortium outperformed individual and the Halorhabdus sp. SVX81 with H. volcanii SVX82 bipartite cultures in xylan degradation, exhibiting a division of labor: the DPANN symbiont processed glycolysis products, while other members performed xylan depolymerization and biosynthesis of essential compounds. Electron microscopy and cryo-electron tomography revealed the formation of heterocellular biofilms interlinked by DPANN cells. The findings demonstrated that DPANN symbionts can interact directly with other members of microbial communities, which are not their primary hosts, influencing their gene expression. However, DPANN proliferation requires their primary host presence. The study highlights the collective contribution of consortium members to xylan degradation and their potential for biotechnological applications in the management of hypersaline environments. © 2025 The Author(s)Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/425666https://www.scopus.com/inward/record.uri?eid=2-s2.0-85215853352&doi=10.1016%2Fj.isci.2025.111749&partnerID=40&md5=9c7777073c1daea0db115d97b9b59f8dreponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.isci.2025.111749https://doi.org/10.1016/j.isci.2025.111749Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4256662026-05-22T06:33:51Z
dc.title.none.fl_str_mv DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
title DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
spellingShingle DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
Reva, O.N.
Biochemistry
Environmental Biotechnology
Microbiology
title_short DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
title_full DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
title_fullStr DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
title_full_unstemmed DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
title_sort DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp
dc.creator.none.fl_str_mv Reva, O.N.
La Cono, V.
Marturano, L.
Crisafi, F.
Smedile, F.
Mudaliyar, M.
Ghosal, D.
Selivanova, E.A.
Ignatenko, M.E.
Ferrer, Miguel
Fernandez-Lopez, L.
Krupovic, M.
Yakimov, M.M.
author Reva, O.N.
author_facet Reva, O.N.
La Cono, V.
Marturano, L.
Crisafi, F.
Smedile, F.
Mudaliyar, M.
Ghosal, D.
Selivanova, E.A.
Ignatenko, M.E.
Ferrer, Miguel
Fernandez-Lopez, L.
Krupovic, M.
Yakimov, M.M.
author_role author
author2 La Cono, V.
Marturano, L.
Crisafi, F.
Smedile, F.
Mudaliyar, M.
Ghosal, D.
Selivanova, E.A.
Ignatenko, M.E.
Ferrer, Miguel
Fernandez-Lopez, L.
Krupovic, M.
Yakimov, M.M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biochemistry
Environmental Biotechnology
Microbiology
topic Biochemistry
Environmental Biotechnology
Microbiology
description This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics demonstrated that the tripartite consortium outperformed individual and the Halorhabdus sp. SVX81 with H. volcanii SVX82 bipartite cultures in xylan degradation, exhibiting a division of labor: the DPANN symbiont processed glycolysis products, while other members performed xylan depolymerization and biosynthesis of essential compounds. Electron microscopy and cryo-electron tomography revealed the formation of heterocellular biofilms interlinked by DPANN cells. The findings demonstrated that DPANN symbionts can interact directly with other members of microbial communities, which are not their primary hosts, influencing their gene expression. However, DPANN proliferation requires their primary host presence. The study highlights the collective contribution of consortium members to xylan degradation and their potential for biotechnological applications in the management of hypersaline environments. © 2025 The Author(s)
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/425666
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85215853352&doi=10.1016%2Fj.isci.2025.111749&partnerID=40&md5=9c7777073c1daea0db115d97b9b59f8d
url http://hdl.handle.net/10261/425666
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85215853352&doi=10.1016%2Fj.isci.2025.111749&partnerID=40&md5=9c7777073c1daea0db115d97b9b59f8d
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1016/j.isci.2025.111749
https://doi.org/10.1016/j.isci.2025.111749
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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