DPANN symbiont of Haloferax volcanii accelerates xylan degradation by the non-host haloarchaeon Halorhabdus sp

This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics dem...

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Detalles Bibliográficos
Autores: Reva, O.N., La Cono, V., Marturano, L., Crisafi, F., Smedile, F., Mudaliyar, M., Ghosal, D., Selivanova, E.A., Ignatenko, M.E., Ferrer, Miguel, Fernandez-Lopez, L., Krupovic, M., Yakimov, M.M.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/425666
Acceso en línea:http://hdl.handle.net/10261/425666
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85215853352&doi=10.1016%2Fj.isci.2025.111749&partnerID=40&md5=9c7777073c1daea0db115d97b9b59f8d
Access Level:acceso abierto
Palabra clave:Biochemistry
Environmental Biotechnology
Microbiology
Descripción
Sumario:This study examines a natural consortium of halophilic archaea, comprising xylan-degrading Halorhabdus sp. SVX81, consortium cohabitant Haloferax volcanii SVX82 (formerly H. lucentense SVX82), and its DPANN ectosymbiont Ca. Nanohalococcus occultus SVXNc. Transcriptomics and targeted metabolomics demonstrated that the tripartite consortium outperformed individual and the Halorhabdus sp. SVX81 with H. volcanii SVX82 bipartite cultures in xylan degradation, exhibiting a division of labor: the DPANN symbiont processed glycolysis products, while other members performed xylan depolymerization and biosynthesis of essential compounds. Electron microscopy and cryo-electron tomography revealed the formation of heterocellular biofilms interlinked by DPANN cells. The findings demonstrated that DPANN symbionts can interact directly with other members of microbial communities, which are not their primary hosts, influencing their gene expression. However, DPANN proliferation requires their primary host presence. The study highlights the collective contribution of consortium members to xylan degradation and their potential for biotechnological applications in the management of hypersaline environments. © 2025 The Author(s)

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