Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20

Bacterial conjugation is the main horizontal gene transfer route responsible for the spread of antibiotic resistance, virulence and toxin genes. During conjugation, DNA is transferred from a donor to a recipient cell via a sophisticated channel connecting the two cells. Conjugation not only affects...

ver descrição completa

Detalhes bibliográficos
Autores: Meijer, Wilfried J. J.|||0000-0003-1842-0049, Boer, Roeland|||0000-0001-5949-6627, Ares, Saúl, Alfonso, Carlos|||0000-0001-7165-4800, Rojo, Fernando|||0000-0003-1848-7745, Luque-Ortega, Juan Román|||0000-0003-3206-7480, Wu, Ling Juan
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:238544
Acesso em linha:https://ddd.uab.cat/record/238544
https://dx.doi.org/urn:doi:10.3389/fmolb.2021.648468
Access Level:acceso abierto
Palavra-chave:Quorum sensing
Bacillus subtilis
Gram-positive bacteria
Antibiotic resistance
Horizontal gene transfer
DNA looping
Transcriptional regulation
Conjugation
id ES_b43609c17e6e9dff7261dfd1374b46b3
oai_identifier_str oai:ddd.uab.cat:238544
network_acronym_str ES
network_name_str España
repository_id_str
spelling Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20Meijer, Wilfried J. J.|||0000-0003-1842-0049Boer, Roeland|||0000-0001-5949-6627Ares, SaúlAlfonso, Carlos|||0000-0001-7165-4800Rojo, Fernando|||0000-0003-1848-7745Luque-Ortega, Juan Román|||0000-0003-3206-7480Wu, Ling JuanQuorum sensingBacillus subtilisGram-positive bacteriaAntibiotic resistanceHorizontal gene transferDNA loopingTranscriptional regulationConjugationBacterial conjugation is the main horizontal gene transfer route responsible for the spread of antibiotic resistance, virulence and toxin genes. During conjugation, DNA is transferred from a donor to a recipient cell via a sophisticated channel connecting the two cells. Conjugation not only affects many different aspects of the plasmid and the host, ranging from the properties of the membrane and the cell surface of the donor, to other developmental processes such as competence, it probably also poses a burden on the donor cell due to the expression of the large number of genes involved in the conjugation process. Therefore, expression of the conjugation genes must be strictly controlled. Over the past decade, the regulation of the conjugation genes present on the conjugative Bacillus subtilis plasmid pLS20 has been studied using a variety of methods including genetic, biochemical, biophysical and structural approaches. This review focuses on the interplay between Rco, Rap and Phr*, the proteins that control the activity of the main conjugation promoter P located upstream of the conjugation operon. Proper expression of the conjugation genes requires the following two fundamental elements. First, conjugation is repressed by default and an intercellular quorum-signaling system is used to sense conditions favorable for conjugation. Second, different layers of regulation act together to repress the P promoter in a strict manner but allowing rapid activation. During conjugation, ssDNA is exported from the cell by a membrane-embedded DNA translocation machine. Another membrane-embedded DNA translocation machine imports ssDNA in competent cells. Evidences are reviewed indicating that conjugation and competence are probably mutually exclusive processes. Some of the questions that remain unanswered are discussed. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/238544https://dx.doi.org/urn:doi:10.3389/fmolb.2021.648468reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-108778GB-C21Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2016-77883-C2-2-PMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 FIS2015-72574-EXPAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104544GB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-109320GB-100Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FIS2016-78313-PAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 SEV-2017-0712Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PGC2018-094143-B-I00open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2385442026-06-06T12:50:31Z
dc.title.none.fl_str_mv Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
title Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
spellingShingle Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
Meijer, Wilfried J. J.|||0000-0003-1842-0049
Quorum sensing
Bacillus subtilis
Gram-positive bacteria
Antibiotic resistance
Horizontal gene transfer
DNA looping
Transcriptional regulation
Conjugation
title_short Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
title_full Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
title_fullStr Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
title_full_unstemmed Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
title_sort Multiple Layered Control of the Conjugation Process of the Bacillus subtilis Plasmid pLS20
dc.creator.none.fl_str_mv Meijer, Wilfried J. J.|||0000-0003-1842-0049
Boer, Roeland|||0000-0001-5949-6627
Ares, Saúl
Alfonso, Carlos|||0000-0001-7165-4800
Rojo, Fernando|||0000-0003-1848-7745
Luque-Ortega, Juan Román|||0000-0003-3206-7480
Wu, Ling Juan
author Meijer, Wilfried J. J.|||0000-0003-1842-0049
author_facet Meijer, Wilfried J. J.|||0000-0003-1842-0049
Boer, Roeland|||0000-0001-5949-6627
Ares, Saúl
Alfonso, Carlos|||0000-0001-7165-4800
Rojo, Fernando|||0000-0003-1848-7745
Luque-Ortega, Juan Román|||0000-0003-3206-7480
Wu, Ling Juan
author_role author
author2 Boer, Roeland|||0000-0001-5949-6627
Ares, Saúl
Alfonso, Carlos|||0000-0001-7165-4800
Rojo, Fernando|||0000-0003-1848-7745
Luque-Ortega, Juan Román|||0000-0003-3206-7480
Wu, Ling Juan
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Quorum sensing
Bacillus subtilis
Gram-positive bacteria
Antibiotic resistance
Horizontal gene transfer
DNA looping
Transcriptional regulation
Conjugation
topic Quorum sensing
Bacillus subtilis
Gram-positive bacteria
Antibiotic resistance
Horizontal gene transfer
DNA looping
Transcriptional regulation
Conjugation
description Bacterial conjugation is the main horizontal gene transfer route responsible for the spread of antibiotic resistance, virulence and toxin genes. During conjugation, DNA is transferred from a donor to a recipient cell via a sophisticated channel connecting the two cells. Conjugation not only affects many different aspects of the plasmid and the host, ranging from the properties of the membrane and the cell surface of the donor, to other developmental processes such as competence, it probably also poses a burden on the donor cell due to the expression of the large number of genes involved in the conjugation process. Therefore, expression of the conjugation genes must be strictly controlled. Over the past decade, the regulation of the conjugation genes present on the conjugative Bacillus subtilis plasmid pLS20 has been studied using a variety of methods including genetic, biochemical, biophysical and structural approaches. This review focuses on the interplay between Rco, Rap and Phr*, the proteins that control the activity of the main conjugation promoter P located upstream of the conjugation operon. Proper expression of the conjugation genes requires the following two fundamental elements. First, conjugation is repressed by default and an intercellular quorum-signaling system is used to sense conditions favorable for conjugation. Second, different layers of regulation act together to repress the P promoter in a strict manner but allowing rapid activation. During conjugation, ssDNA is exported from the cell by a membrane-embedded DNA translocation machine. Another membrane-embedded DNA translocation machine imports ssDNA in competent cells. Evidences are reviewed indicating that conjugation and competence are probably mutually exclusive processes. Some of the questions that remain unanswered are discussed.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/238544
https://dx.doi.org/urn:doi:10.3389/fmolb.2021.648468
url https://ddd.uab.cat/record/238544
https://dx.doi.org/urn:doi:10.3389/fmolb.2021.648468
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-108778GB-C21
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2016-77883-C2-2-P
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FIS2015-72574-EXP
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104544GB-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-109320GB-100
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 FIS2016-78313-P
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SEV-2017-0712
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PGC2018-094143-B-I00
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869417241619988480
score 15,300719

Registos relacionados