Rapid conjugative mobilization of a 100kb segment of Bacillus subtilis chromosomal DNA is mediated by a helper plasmid with no ability for self-transfer
Background: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. Results: In this study, the oriT s...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/181643 |
| Acceso en línea: | http://hdl.handle.net/10261/181643 |
| Access Level: | acceso abierto |
| Palabra clave: | Gram positive Conjugation Bacillus subtilis Plasmid transfer |
| Sumario: | Background: The conjugative plasmid, pLS20, isolated from Bacillus subtilis natto, has an outstanding capacity for rapid self-transfer. In addition, it can function as a helper plasmid, mediating the mobilization of an independently replicating co-resident plasmid. Results: In this study, the oriT sequence of pLS20cat (oriT ) was eliminated to obtain the plasmid, pLS20catoriT. This resulted in the complete loss of the conjugative transfer of the plasmid but still allowed it to mobilize a co-resident mobilizable plasmid. Moreover, pLS20catoriT was able to mobilize longer DNA segments, up to 113kb of chromosomal DNA containing oriT , after mixing the liquid cultures of the donor and recipient for only 15min. Conclusions: The chromosomal DNA mobilization mediated by pLS20catoriT will allow us to develop a novel genetic tool for the rapid, easy, and repetitive mobilization of longer DNA segments into a recipient chromosome. |
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