Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital

Studies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. The aim of this work is to describe the bacterial communities inhabiting the gastric content (GC) of preterm neonates. For that purpose, GC samples were collected weekly from a tota...

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Autores: Moles, L, Gomez, M, Jimenez, E, Bustos, G, de Andres, J, Melgar, A, Escuder, D, Fernandez, L, del Campo, R, Rodriguez, JM
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p10391
Acceso en línea:https://fisabio.portalinvestigacion.com/publicaciones/10391
Access Level:acceso abierto
Palabra clave:preterm infants
stomach
gastric content
microbiome
microbiota
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spelling Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the HospitalMoles, LGomez, MJimenez, EBustos, Gde Andres, JMelgar, AEscuder, DFernandez, Ldel Campo, RRodriguez, JMpreterm infantsstomachgastric contentmicrobiomemicrobiotaStudies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. The aim of this work is to describe the bacterial communities inhabiting the gastric content (GC) of preterm neonates. For that purpose, GC samples were collected weekly from a total of 13 preterm neonates during their first month of life within their hospital stay. Samples were analyzed by using both culture-dependent and -independent techniques. The former allowed the isolation of bacteria belonging mainly to the genera Enterococcus, Staphylococcus, Streptococcus, Serratia, Klebsiella, and Escherichia. The cultured dominant species in the GC samples during all the hospitalization period were Enterococcus faecalis and Staphylococcus epidermidis. Multilocus sequence typing (MLST) analysis revealed the presence of high-risk clonal complexes associated with the hospital environment, which may colonize enteral feeding tubes. Similarly, the 16S rRNA sequencing showed that Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Corynebacterium, and Propionibacterium were the dominant genera present at 75% of the gastric samples. However, the genera Serratia, Klebsiella, and Streptococcus were the most abundant. Own mother's milk (OMM) and donor milk (DM) were collected after their pass through the external feeding tubes to assess their bacterial content. OMM and DM had a similar bacterial pattern to GC. Based on these data, the GC of preterm neonates is dominated by Proteobacteria and Firmicutes and harbors high-risk bacterial clones, which may colonize enteral feeding tubes, and therefore the feeds that pass through them.FRONTIERS MEDIA SA2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/10391Frontiers in NutritionISSN: 2296861Xreponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p103912026-06-11T12:45:17Z
dc.title.none.fl_str_mv Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
title Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
spellingShingle Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
Moles, L
preterm infants
stomach
gastric content
microbiome
microbiota
title_short Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
title_full Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
title_fullStr Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
title_full_unstemmed Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
title_sort Bacterial Diversity of the Gastric Content of Preterm Infants during Their First Month of Life at the Hospital
dc.creator.none.fl_str_mv Moles, L
Gomez, M
Jimenez, E
Bustos, G
de Andres, J
Melgar, A
Escuder, D
Fernandez, L
del Campo, R
Rodriguez, JM
author Moles, L
author_facet Moles, L
Gomez, M
Jimenez, E
Bustos, G
de Andres, J
Melgar, A
Escuder, D
Fernandez, L
del Campo, R
Rodriguez, JM
author_role author
author2 Gomez, M
Jimenez, E
Bustos, G
de Andres, J
Melgar, A
Escuder, D
Fernandez, L
del Campo, R
Rodriguez, JM
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv preterm infants
stomach
gastric content
microbiome
microbiota
topic preterm infants
stomach
gastric content
microbiome
microbiota
description Studies focused on the stomach microbiota are relatively scarce, and most of them are focused on the adult population. The aim of this work is to describe the bacterial communities inhabiting the gastric content (GC) of preterm neonates. For that purpose, GC samples were collected weekly from a total of 13 preterm neonates during their first month of life within their hospital stay. Samples were analyzed by using both culture-dependent and -independent techniques. The former allowed the isolation of bacteria belonging mainly to the genera Enterococcus, Staphylococcus, Streptococcus, Serratia, Klebsiella, and Escherichia. The cultured dominant species in the GC samples during all the hospitalization period were Enterococcus faecalis and Staphylococcus epidermidis. Multilocus sequence typing (MLST) analysis revealed the presence of high-risk clonal complexes associated with the hospital environment, which may colonize enteral feeding tubes. Similarly, the 16S rRNA sequencing showed that Streptococcus, Staphylococcus, Lactobacillus, Enterococcus, Corynebacterium, and Propionibacterium were the dominant genera present at 75% of the gastric samples. However, the genera Serratia, Klebsiella, and Streptococcus were the most abundant. Own mother's milk (OMM) and donor milk (DM) were collected after their pass through the external feeding tubes to assess their bacterial content. OMM and DM had a similar bacterial pattern to GC. Based on these data, the GC of preterm neonates is dominated by Proteobacteria and Firmicutes and harbors high-risk bacterial clones, which may colonize enteral feeding tubes, and therefore the feeds that pass through them.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/10391
url https://fisabio.portalinvestigacion.com/publicaciones/10391
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv FRONTIERS MEDIA SA
publisher.none.fl_str_mv FRONTIERS MEDIA SA
dc.source.none.fl_str_mv Frontiers in Nutrition
ISSN: 2296861X
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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