Securin Is a Target of the UV Response Pathway in Mammalian Cells

All eukaryotic cells possess elaborate mechanisms to protect genome integrity and ensure survival after DNA damage, ceasing proliferation and granting time for DNA repair. Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the on...

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Detalhes bibliográficos
Autores: Romero Portillo, Francisco, Gil-Bernabé, Ana M., Sáez, Carmen, Pintor Toro, José Antonio, Tortolero García, María Dolores
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2004
País:España
Recursos:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/70790
Acesso em linha:https://hdl.handle.net/11441/70790
https://doi.org/10.1128/MCB.24.7.2720-2733.2004
Access Level:acceso abierto
Palavra-chave:Cell DNA
Messenger RNA
Unclassified drug
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spelling Securin Is a Target of the UV Response Pathway in Mammalian CellsRomero Portillo, FranciscoGil-Bernabé, Ana M.Sáez, CarmenPintor Toro, José AntonioTortolero García, María DoloresCell DNAMessenger RNAUnclassified drugAll eukaryotic cells possess elaborate mechanisms to protect genome integrity and ensure survival after DNA damage, ceasing proliferation and granting time for DNA repair. Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the onset of anaphase. At the metaphase-to-anaphase transition, Securin is degraded by the anaphase-promoting complex or cyclosome, and Separase contributes to the release of cohesins from the chromosome, allowing for the segregation of sister chromatids to opposite spindle poles. Here we provide evidence that human Securin (hSecurin) has a novel role in cell cycle arrest after exposure to UV light or ionizing radiation. In fact, irradiation downregulated the level of hSecurin protein, accelerating its degradation via the proteasome and reducing hSecurin mRNA translation, but the presence of hSecurin is necessary for cell proliferation arrest following UV treatment. Moreover, an alteration of UV-induced hSecurin downregulation could lead directly to the accumulation of DNA damage and the subsequent development of malignant tumors.Ministerio de Ciencia y Tecnología SAF2002-04177-C04Junta de Andalucía DGUIMicrobiologíaCitología e Histología Normal y Patológica Ministerio de Ciencia y Tecnología (MCYT). EspañaJunta de Andalucía2004info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/70790https://doi.org/10.1128/MCB.24.7.2720-2733.2004reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular and Cellular Biology, 24 (7), 2720-2733.http://dx.doi.org/10.1128/MCB.24.7.2720-2733.2004info:eu-repo/semantics/openAccessoai:idus.us.es:11441/707902026-06-17T12:51:07Z
dc.title.none.fl_str_mv Securin Is a Target of the UV Response Pathway in Mammalian Cells
title Securin Is a Target of the UV Response Pathway in Mammalian Cells
spellingShingle Securin Is a Target of the UV Response Pathway in Mammalian Cells
Romero Portillo, Francisco
Cell DNA
Messenger RNA
Unclassified drug
title_short Securin Is a Target of the UV Response Pathway in Mammalian Cells
title_full Securin Is a Target of the UV Response Pathway in Mammalian Cells
title_fullStr Securin Is a Target of the UV Response Pathway in Mammalian Cells
title_full_unstemmed Securin Is a Target of the UV Response Pathway in Mammalian Cells
title_sort Securin Is a Target of the UV Response Pathway in Mammalian Cells
dc.creator.none.fl_str_mv Romero Portillo, Francisco
Gil-Bernabé, Ana M.
Sáez, Carmen
Pintor Toro, José Antonio
Tortolero García, María Dolores
author Romero Portillo, Francisco
author_facet Romero Portillo, Francisco
Gil-Bernabé, Ana M.
Sáez, Carmen
Pintor Toro, José Antonio
Tortolero García, María Dolores
author_role author
author2 Gil-Bernabé, Ana M.
Sáez, Carmen
Pintor Toro, José Antonio
Tortolero García, María Dolores
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Microbiología
Citología e Histología Normal y Patológica
Ministerio de Ciencia y Tecnología (MCYT). España
Junta de Andalucía
dc.subject.none.fl_str_mv Cell DNA
Messenger RNA
Unclassified drug
topic Cell DNA
Messenger RNA
Unclassified drug
description All eukaryotic cells possess elaborate mechanisms to protect genome integrity and ensure survival after DNA damage, ceasing proliferation and granting time for DNA repair. Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the onset of anaphase. At the metaphase-to-anaphase transition, Securin is degraded by the anaphase-promoting complex or cyclosome, and Separase contributes to the release of cohesins from the chromosome, allowing for the segregation of sister chromatids to opposite spindle poles. Here we provide evidence that human Securin (hSecurin) has a novel role in cell cycle arrest after exposure to UV light or ionizing radiation. In fact, irradiation downregulated the level of hSecurin protein, accelerating its degradation via the proteasome and reducing hSecurin mRNA translation, but the presence of hSecurin is necessary for cell proliferation arrest following UV treatment. Moreover, an alteration of UV-induced hSecurin downregulation could lead directly to the accumulation of DNA damage and the subsequent development of malignant tumors.
publishDate 2004
dc.date.none.fl_str_mv 2004
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/70790
https://doi.org/10.1128/MCB.24.7.2720-2733.2004
url https://hdl.handle.net/11441/70790
https://doi.org/10.1128/MCB.24.7.2720-2733.2004
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular and Cellular Biology, 24 (7), 2720-2733.
http://dx.doi.org/10.1128/MCB.24.7.2720-2733.2004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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