Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent

Lung cancer (LC) is a leading cause of mortality, claiming more than 1.8 million deaths per year worldwide. Surgery is one of the most effective treatments when the disease is in its early stages. The study of metabolic alterations after surgical intervention with curative intent could be used to as...

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Detalles Bibliográficos
Autores: Sánchez Espirilla, Saida, Pereira Vega, Antonio, Callejón Leblic, María Belén, Díaz Olivares, Isabel, Gómez Ariza, José Luis, García Barrera, Tamara
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Huelva (UHU)
Repositorio:Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:inglés
OAI Identifier:oai:ariasmontano.uhu.es:10272/22612
Acceso en línea:https://hdl.handle.net/10272/22612
Access Level:acceso abierto
Palabra clave:Ultra-high performance liquid chromatography
Metabolomics
Mass spectrometry
Hydrophilicinteraction
Lung cancer
Serum
Surgery
23 Química
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spelling Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative IntentSánchez Espirilla, SaidaPereira Vega, AntonioCallejón Leblic, María BelénDíaz Olivares, IsabelGómez Ariza, José LuisGarcía Barrera, TamaraUltra-high performance liquid chromatographyMetabolomicsMass spectrometryHydrophilicinteractionLung cancerSerumSurgery23 QuímicaLung cancer (LC) is a leading cause of mortality, claiming more than 1.8 million deaths per year worldwide. Surgery is one of the most effective treatments when the disease is in its early stages. The study of metabolic alterations after surgical intervention with curative intent could be used to assess the response to treatment or the detection of cancer recurrence. In this study, we have evaluated the metabolomic profile of serum samples (n = 110) from preoperative (PRE) and postoperative (POST) LC patients collected at two different time points (1 month, A; 3-6 months, B) with respect to healthy people. An untargeted metabolomic platform based on reversed phase (RP) and hydrophilic interaction chromatography (HILIC), using ultra-high performance liquid chromatography (UHPLC) and mass spectrometry (MS), was applied (MassIVE ID MSV000092213). Twenty-two altered metabolites were annotated by comparing all the different studied groups. DG(14,0/22:1), stearamide, proline, and E,e-carotene-3,3 '-dione were found altered in PRE, and their levels returned to those of a baseline control group 3-6 months after surgery. Furthermore, 3-galactosyllactose levels remained altered after intervention in some patients. This study provides unique insights into the metabolic profiles of LC patients after surgery at two different time points by combining complementary analytical methodsAmerican Chemical Society20232023-10-0120232023-10-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/22612reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/226122026-06-02T14:58:11Z
dc.title.none.fl_str_mv Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
title Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
spellingShingle Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
Sánchez Espirilla, Saida
Ultra-high performance liquid chromatography
Metabolomics
Mass spectrometry
Hydrophilicinteraction
Lung cancer
Serum
Surgery
23 Química
title_short Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
title_full Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
title_fullStr Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
title_full_unstemmed Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
title_sort Untargeted Metabolomic Study of Lung Cancer Patients after Surgery with Curative Intent
dc.creator.none.fl_str_mv Sánchez Espirilla, Saida
Pereira Vega, Antonio
Callejón Leblic, María Belén
Díaz Olivares, Isabel
Gómez Ariza, José Luis
García Barrera, Tamara
author Sánchez Espirilla, Saida
author_facet Sánchez Espirilla, Saida
Pereira Vega, Antonio
Callejón Leblic, María Belén
Díaz Olivares, Isabel
Gómez Ariza, José Luis
García Barrera, Tamara
author_role author
author2 Pereira Vega, Antonio
Callejón Leblic, María Belén
Díaz Olivares, Isabel
Gómez Ariza, José Luis
García Barrera, Tamara
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Ultra-high performance liquid chromatography
Metabolomics
Mass spectrometry
Hydrophilicinteraction
Lung cancer
Serum
Surgery
23 Química
topic Ultra-high performance liquid chromatography
Metabolomics
Mass spectrometry
Hydrophilicinteraction
Lung cancer
Serum
Surgery
23 Química
description Lung cancer (LC) is a leading cause of mortality, claiming more than 1.8 million deaths per year worldwide. Surgery is one of the most effective treatments when the disease is in its early stages. The study of metabolic alterations after surgical intervention with curative intent could be used to assess the response to treatment or the detection of cancer recurrence. In this study, we have evaluated the metabolomic profile of serum samples (n = 110) from preoperative (PRE) and postoperative (POST) LC patients collected at two different time points (1 month, A; 3-6 months, B) with respect to healthy people. An untargeted metabolomic platform based on reversed phase (RP) and hydrophilic interaction chromatography (HILIC), using ultra-high performance liquid chromatography (UHPLC) and mass spectrometry (MS), was applied (MassIVE ID MSV000092213). Twenty-two altered metabolites were annotated by comparing all the different studied groups. DG(14,0/22:1), stearamide, proline, and E,e-carotene-3,3 '-dione were found altered in PRE, and their levels returned to those of a baseline control group 3-6 months after surgery. Furthermore, 3-galactosyllactose levels remained altered after intervention in some patients. This study provides unique insights into the metabolic profiles of LC patients after surgery at two different time points by combining complementary analytical methods
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-10-01
2023
2023-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10272/22612
url https://hdl.handle.net/10272/22612
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva
instname:Universidad de Huelva (UHU)
instname_str Universidad de Huelva (UHU)
reponame_str Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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