Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment

Pulmonary surfactant protein C (SP-C) may play a key role in alveolar homeostasis by modulating vesicle uptake in alveolar cells. This study explores how SP-C regulates internalization of model unilamellar lipid vesicles by type II alveolar epithelial cells (AECII) and alveolar macrophages (AMϕ), fo...

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Detalles Bibliográficos
Autores: Morán Lalangui, Juranny Michelle, Villoslada González, Marta, Hevia Lorenzo, Carmen, Pérez Gil, Jesús, García Álvarez, María Begoña
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/129300
Acceso en línea:https://hdl.handle.net/20.500.14352/129300
Access Level:acceso abierto
Palabra clave:616.24
577.1
577.27
576
SP-C
Lipid vesicle uptake
Palmitoylation
Alveolar like cells
SP-B
LPS
Neumología
Bioquímica (Biología)
Biología molecular (Biología)
Biología celular (Biología)
Inmunología
3205.08 Enfermedades Pulmonares
2403 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2412 Inmunología
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oai_identifier_str oai:docta.ucm.es:20.500.14352/129300
network_acronym_str ES
network_name_str España
repository_id_str
spelling Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environmentMorán Lalangui, Juranny MichelleVilloslada González, MartaHevia Lorenzo, CarmenPérez Gil, JesúsGarcía Álvarez, María Begoña616.24577.1577.27576SP-CLipid vesicle uptakePalmitoylationAlveolar like cellsSP-BLPSNeumologíaBioquímica (Biología)Biología molecular (Biología)Biología celular (Biología)Inmunología3205.08 Enfermedades Pulmonares2403 Bioquímica2415 Biología Molecular2407 Biología Celular2412 InmunologíaPulmonary surfactant protein C (SP-C) may play a key role in alveolar homeostasis by modulating vesicle uptake in alveolar cells. This study explores how SP-C regulates internalization of model unilamellar lipid vesicles by type II alveolar epithelial cells (AECII) and alveolar macrophages (AMϕ), focusing on the effect of lipid composition, palmitoylation state, and interactions with external stimuli like lipopolysaccharides (LPS) or the other hydrophobic surfactant protein SP-B. Using fluorescence-based techniques, we demonstrated that SP-C enhances vesicle uptake in a lipid-dependent manner. While AECII internalize vesicles regardless of lipid composition, AMϕ show a preference for vesicles with specific lipid profiles. The palmitoylation of SP-C is essential for efficient vesicle uptake, highlighting the importance of membrane-protein interactions in this process. Furthermore, SP-C colocalizes with acidic organelles within both cell types, suggesting its involvement in intracellular trafficking and surfactant homeostasis. Notably, SP-C facilitates LPS uptake by AMϕ, potentially contributing to immune modulation in the alveolar spaces. The contribution of SP-C to metabolism and pulmonary immunity has important implications in lung diseases involving surfactant dysfunction or immune dysregulation.ElsevierUniversidad Complutense de Madrid20252025-12-0120252025-12-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/129300reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengMICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-124932OB-100MICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-094564-B-100MICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2024-2027 PID2024-156556OB-100open accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1293002026-06-02T12:44:21Z
dc.title.none.fl_str_mv Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
title Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
spellingShingle Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
Morán Lalangui, Juranny Michelle
616.24
577.1
577.27
576
SP-C
Lipid vesicle uptake
Palmitoylation
Alveolar like cells
SP-B
LPS
Neumología
Bioquímica (Biología)
Biología molecular (Biología)
Biología celular (Biología)
Inmunología
3205.08 Enfermedades Pulmonares
2403 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2412 Inmunología
title_short Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
title_full Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
title_fullStr Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
title_full_unstemmed Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
title_sort Pulmonary surfactant protein SP-C regulates lipid vesicle uptake by alveolar type II cells and macrophages: Role of lipids, palmitoylation, and environment
dc.creator.none.fl_str_mv Morán Lalangui, Juranny Michelle
Villoslada González, Marta
Hevia Lorenzo, Carmen
Pérez Gil, Jesús
García Álvarez, María Begoña
author Morán Lalangui, Juranny Michelle
author_facet Morán Lalangui, Juranny Michelle
Villoslada González, Marta
Hevia Lorenzo, Carmen
Pérez Gil, Jesús
García Álvarez, María Begoña
author_role author
author2 Villoslada González, Marta
Hevia Lorenzo, Carmen
Pérez Gil, Jesús
García Álvarez, María Begoña
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 616.24
577.1
577.27
576
SP-C
Lipid vesicle uptake
Palmitoylation
Alveolar like cells
SP-B
LPS
Neumología
Bioquímica (Biología)
Biología molecular (Biología)
Biología celular (Biología)
Inmunología
3205.08 Enfermedades Pulmonares
2403 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2412 Inmunología
topic 616.24
577.1
577.27
576
SP-C
Lipid vesicle uptake
Palmitoylation
Alveolar like cells
SP-B
LPS
Neumología
Bioquímica (Biología)
Biología molecular (Biología)
Biología celular (Biología)
Inmunología
3205.08 Enfermedades Pulmonares
2403 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2412 Inmunología
description Pulmonary surfactant protein C (SP-C) may play a key role in alveolar homeostasis by modulating vesicle uptake in alveolar cells. This study explores how SP-C regulates internalization of model unilamellar lipid vesicles by type II alveolar epithelial cells (AECII) and alveolar macrophages (AMϕ), focusing on the effect of lipid composition, palmitoylation state, and interactions with external stimuli like lipopolysaccharides (LPS) or the other hydrophobic surfactant protein SP-B. Using fluorescence-based techniques, we demonstrated that SP-C enhances vesicle uptake in a lipid-dependent manner. While AECII internalize vesicles regardless of lipid composition, AMϕ show a preference for vesicles with specific lipid profiles. The palmitoylation of SP-C is essential for efficient vesicle uptake, highlighting the importance of membrane-protein interactions in this process. Furthermore, SP-C colocalizes with acidic organelles within both cell types, suggesting its involvement in intracellular trafficking and surfactant homeostasis. Notably, SP-C facilitates LPS uptake by AMϕ, potentially contributing to immune modulation in the alveolar spaces. The contribution of SP-C to metabolism and pulmonary immunity has important implications in lung diseases involving surfactant dysfunction or immune dysregulation.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-12-01
2025
2025-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/129300
url https://hdl.handle.net/20.500.14352/129300
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-124932OB-100
MICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-094564-B-100
MICIU Plan Estatal de Investigación Científica y Técnica y de Innovación 2024-2027 PID2024-156556OB-100
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial 4.0 International
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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