Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host

Classical swine fever virus (CSFV) remains a highly important pathogen, causing major losses in the swine industry. Persistent infection is highly relevant for CSFV maintenance in the field; however, this form of infection is not fully understood. An increase in the granulocyte population has been d...

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Autores: Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757, Muñoz-González, Sara, Pérez-Simó, Marta, Revilla, Concepción, Domínguez, Javier|||0000-0001-7256-388X, Ganges, Llilianne|||0000-0002-8644-3560
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:253335
Acesso em linha:https://ddd.uab.cat/record/253335
https://dx.doi.org/urn:doi:10.3390/v11090822
Access Level:acceso abierto
Palavra-chave:CSFV
Viral persistence
Immunosuppression
Peripheral blood
Bone marrow cells
6D10 cells
MDSC
Interferon gamma
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spelling Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the HostBohórquez Garzón, José Alejandro|||0000-0002-6715-1757Muñoz-González, SaraPérez-Simó, MartaRevilla, ConcepciónDomínguez, Javier|||0000-0001-7256-388XGanges, Llilianne|||0000-0002-8644-3560CSFVViral persistenceImmunosuppressionPeripheral bloodBone marrow cells6D10 cellsMDSCInterferon gammaClassical swine fever virus (CSFV) remains a highly important pathogen, causing major losses in the swine industry. Persistent infection is highly relevant for CSFV maintenance in the field; however, this form of infection is not fully understood. An increase in the granulocyte population has been detected in CSFV persistently infected animals. The aim of this work was to evaluate the possible immunosuppressive role of these cells in CSFV persistent infection. The phenotype of peripheral blood and bone marrow cells from persistently infected and naïve animals was evaluated by flow cytometry, and the capacity of specific cell subsets to reduce the interferon gamma (IFN-γ) response against unspecific and specific antigen was determined using co-culture assays. The frequency of granulocytic cells was increased in cells from CSFV persistently infected pigs and they showed a phenotype similar to immunosuppressive cell populations found in persistent infection in humans. These cells from persistently infected animals were able to reduce the IFN-γ response against unspecific and specific antigen. Our results suggest that immature immunosuppressive cell populations play a role in CSFV persistent infection in swine. The information obtained by studying the role of myeloid derived suppressor cells (MDSC) during CSFV persistent infection may extrapolate to other viral persistent infections in mammals. 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/253335https://dx.doi.org/urn:doi:10.3390/v11090822reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RTI2018-100887-B-100Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 AGL2015-66907Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 FPI2016open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2533352026-06-06T12:50:31Z
dc.title.none.fl_str_mv Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
title Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
spellingShingle Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
CSFV
Viral persistence
Immunosuppression
Peripheral blood
Bone marrow cells
6D10 cells
MDSC
Interferon gamma
title_short Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
title_full Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
title_fullStr Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
title_full_unstemmed Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
title_sort Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host
dc.creator.none.fl_str_mv Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
Muñoz-González, Sara
Pérez-Simó, Marta
Revilla, Concepción
Domínguez, Javier|||0000-0001-7256-388X
Ganges, Llilianne|||0000-0002-8644-3560
author Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
author_facet Bohórquez Garzón, José Alejandro|||0000-0002-6715-1757
Muñoz-González, Sara
Pérez-Simó, Marta
Revilla, Concepción
Domínguez, Javier|||0000-0001-7256-388X
Ganges, Llilianne|||0000-0002-8644-3560
author_role author
author2 Muñoz-González, Sara
Pérez-Simó, Marta
Revilla, Concepción
Domínguez, Javier|||0000-0001-7256-388X
Ganges, Llilianne|||0000-0002-8644-3560
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CSFV
Viral persistence
Immunosuppression
Peripheral blood
Bone marrow cells
6D10 cells
MDSC
Interferon gamma
topic CSFV
Viral persistence
Immunosuppression
Peripheral blood
Bone marrow cells
6D10 cells
MDSC
Interferon gamma
description Classical swine fever virus (CSFV) remains a highly important pathogen, causing major losses in the swine industry. Persistent infection is highly relevant for CSFV maintenance in the field; however, this form of infection is not fully understood. An increase in the granulocyte population has been detected in CSFV persistently infected animals. The aim of this work was to evaluate the possible immunosuppressive role of these cells in CSFV persistent infection. The phenotype of peripheral blood and bone marrow cells from persistently infected and naïve animals was evaluated by flow cytometry, and the capacity of specific cell subsets to reduce the interferon gamma (IFN-γ) response against unspecific and specific antigen was determined using co-culture assays. The frequency of granulocytic cells was increased in cells from CSFV persistently infected pigs and they showed a phenotype similar to immunosuppressive cell populations found in persistent infection in humans. These cells from persistently infected animals were able to reduce the IFN-γ response against unspecific and specific antigen. Our results suggest that immature immunosuppressive cell populations play a role in CSFV persistent infection in swine. The information obtained by studying the role of myeloid derived suppressor cells (MDSC) during CSFV persistent infection may extrapolate to other viral persistent infections in mammals.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/253335
https://dx.doi.org/urn:doi:10.3390/v11090822
url https://ddd.uab.cat/record/253335
https://dx.doi.org/urn:doi:10.3390/v11090822
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 RTI2018-100887-B-100
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 AGL2015-66907
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 FPI2016
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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