Quantification of gene expression patterns to reveal the origins of abnormal morphogenesis
The earliest developmental origins of dysmorphologies are poorly understood in many congenital diseases. They often remain elusive because the first signs of genetic misregulation may initiate as subtle changes in gene expression, which are hard to detect and can be obscured later in development by...
| Autores: | , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Conselleria de Salut i Consum del Govern de les Illes Balears |
| Repositorio: | Docusalut |
| Idioma: | inglés |
| OAI Identifier: | oai:docusalut.com:20.500.13003/9120 |
| Acceso en línea: | https://hdl.handle.net/20.500.13003/9120 |
| Access Level: | acceso abierto |
| Palabra clave: | Mice, Inbred C57BL Acrocephalosyndactylia Tomography, X-Ray Computed Receptor, Fibroblast Growth Factor, Type 2 Animals Gene Expression Profiling Gene Expression Regulation, Developmental Biometry Disease Models, Animal Mutation, Missense Receptor Tipo 2 de Factor de Crecimiento de Fibroblastos Animales Tomografía Computarizada por Rayos X Mutación Missense Acrocefalosindactilia Ratones Endogámicos C57BL Biometría Regulación del Desarrollo de la Expresión Génica Perfilación de la Expresión Génica Modelos Animales de Enfermedad |
| Sumario: | The earliest developmental origins of dysmorphologies are poorly understood in many congenital diseases. They often remain elusive because the first signs of genetic misregulation may initiate as subtle changes in gene expression, which are hard to detect and can be obscured later in development by secondary effects. Here, we develop a method to trace back the origins of phenotypic abnormalities by accurately quantifying the 3D spatial distribution of gene expression domains in developing organs. By applying Geometric Morphometrics to 3D gene expression data obtained by Optical Projection Tomography, we determined that our approach is sensitive enough to find regulatory abnormalities that have never been detected previously. We identified subtle but significant differences in the gene expression of a downstream target of a Fgfr2 mutation associated with Apert syndrome, demonstrating that these mouse models can further our understanding of limb defects in the human condition. Our method can be applied to different organ systems and models to investigate the etiology of malformations. |
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