Depletion of T-cell intracellular antigen proteins promotes cell proliferation

Background: T-cell intracellular antigen-1 (TIA-1) and TIA-1 related/like protein (TIAR/TIAL1), two DNA/RNA binding proteins broadly expressed in eukaryotic cells, participate in the regulation of gene expression through RNA metabolism. Despite the biological relevance of these regulators, there are...

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Detalhes bibliográficos
Autores: Reyes, Raquel, Alcalde, José, Izquierdo, José M.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2009
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24244
Acesso em linha:http://hdl.handle.net/10261/24244
Access Level:acceso abierto
Palavra-chave:T-Cell intracellular antigen (TIA)
RNAs
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spelling Depletion of T-cell intracellular antigen proteins promotes cell proliferationReyes, RaquelAlcalde, JoséIzquierdo, José M.T-Cell intracellular antigen (TIA)RNAsBackground: T-cell intracellular antigen-1 (TIA-1) and TIA-1 related/like protein (TIAR/TIAL1), two DNA/RNA binding proteins broadly expressed in eukaryotic cells, participate in the regulation of gene expression through RNA metabolism. Despite the biological relevance of these regulators, there are no genome-wide studies assessing global transcriptomic and phenotypic impacts after changes in the expression and/or function of these proteins.Results: Using high-throughput gene expression profiling, we found that the TIA-1/TIAR-depleted cell phenotype is linked to a transcriptome involved in the control of inflammation, cell-cell signaling, immune-suppression, angiogenesis, metabolism and cell proliferation. Induced genes included pro-inflammatory cytokines, inflammatory chemokines, growth-stimulating factors and pro-angiogenic inducers. Repressed genes involved the RAS oncogene family member RAB40B, regulators of cytoskeleton organization and biogenesis and a mitochondrial modulator. Consistent with these observations, depletion of TIA proteins in HeLa cells results in increased cell proliferation, altered cell-cycle and anchorage-independent growth. Mechanistically, the changes associated with the steady-state target mRNA levels regulated by TIA proteins are consistent with overlapping effects on gene basal transcription rate and mRNA turnover.Conclusions: Collectively, our findings suggest a role for TIA proteins as cellular sensors that modulate gene expression control at the transcriptional and post-transcriptional levels, coupling cell proliferation responses and metabolic homeostasis to cell survival and growth.This work was supported by grants from Fondo de Investigaciones Sanitarias (PI051605) and Ministerio de Ciencia e Innovación (BFU2008-00354). R Reyes is a recipient of a postgraduate fellowship from the Ministerio de Ciencia e Innovación. The CBMSO receives an institutional grant from Fundación Ramón Areces.Peer reviewedBioMed CentralInstituto de Salud Carlos IIIMinisterio de Ciencia e Innovación (España)Fundación Ramón Areces201020102009info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersion959742 bytesapplication/pdfhttp://hdl.handle.net/10261/24244reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1186/gb-2009-10-8-r87info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/242442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Depletion of T-cell intracellular antigen proteins promotes cell proliferation
title Depletion of T-cell intracellular antigen proteins promotes cell proliferation
spellingShingle Depletion of T-cell intracellular antigen proteins promotes cell proliferation
Reyes, Raquel
T-Cell intracellular antigen (TIA)
RNAs
title_short Depletion of T-cell intracellular antigen proteins promotes cell proliferation
title_full Depletion of T-cell intracellular antigen proteins promotes cell proliferation
title_fullStr Depletion of T-cell intracellular antigen proteins promotes cell proliferation
title_full_unstemmed Depletion of T-cell intracellular antigen proteins promotes cell proliferation
title_sort Depletion of T-cell intracellular antigen proteins promotes cell proliferation
dc.creator.none.fl_str_mv Reyes, Raquel
Alcalde, José
Izquierdo, José M.
author Reyes, Raquel
author_facet Reyes, Raquel
Alcalde, José
Izquierdo, José M.
author_role author
author2 Alcalde, José
Izquierdo, José M.
author2_role author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Ministerio de Ciencia e Innovación (España)
Fundación Ramón Areces
dc.subject.none.fl_str_mv T-Cell intracellular antigen (TIA)
RNAs
topic T-Cell intracellular antigen (TIA)
RNAs
description Background: T-cell intracellular antigen-1 (TIA-1) and TIA-1 related/like protein (TIAR/TIAL1), two DNA/RNA binding proteins broadly expressed in eukaryotic cells, participate in the regulation of gene expression through RNA metabolism. Despite the biological relevance of these regulators, there are no genome-wide studies assessing global transcriptomic and phenotypic impacts after changes in the expression and/or function of these proteins.
publishDate 2009
dc.date.none.fl_str_mv 2009
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24244
url http://hdl.handle.net/10261/24244
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1186/gb-2009-10-8-r87
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 959742 bytes
application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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