RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The pH-dependence of RRM3/RNA interaction may have a significant effect in the ov...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/95466 |
| Acceso en línea: | http://hdl.handle.net/10261/95466 |
| Access Level: | acceso abierto |
| Palabra clave: | DNA/RNA binding protein (D/RBP) pH-dependence NMR RNA recognition motif (RRM) T-cell intracellular antigen-1 (TIA-1) |
| Sumario: | Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The pH-dependence of RRM3/RNA interaction may have a significant effect in the overall TIA-1 function. Significance: Unveiling the auxiliary role of RRM3 in RNA recognition sheds light on the TIA-1 function between cell compartments changes due to the protonation / deprotonation of TIA-1 RRM3 histidine residues. The auxiliary role of the Cterminal RRM3 domain in TIA-1 RNA recognition is poorly understood and this work provides an insight into its binding mechanisms |
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