Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex

The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture o...

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Autores: Benabou Zdaou, Sanae, Eritja i Casadellà, Ramon, Gargallo Gómez, Raimundo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/151262
Acceso en línea:https://hdl.handle.net/2445/151262
Access Level:acceso abierto
Palabra clave:Àcids nucleics
G-estructures
Oligonucleòtids
Nucleic acids
G-structures
Oligonucleotides
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spelling Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplexBenabou Zdaou, SanaeEritja i Casadellà, RamonGargallo Gómez, RaimundoÀcids nucleicsG-estructuresOligonucleòtidsNucleic acidsG-structuresOligonucleotidesThe conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.Hindawi2020202020132020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion7 p.application/pdfhttps://hdl.handle.net/2445/151262Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1155/2013/631875ISRN Biochemistry, 2013, vol. 2013, p. 1-7https://doi.org/10.1155/2013/631875cc-by (c) Benabou Zdaou, Sanae et al., 2013http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1512622026-05-29T05:05:01Z
dc.title.none.fl_str_mv Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
title Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
spellingShingle Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
Benabou Zdaou, Sanae
Àcids nucleics
G-estructures
Oligonucleòtids
Nucleic acids
G-structures
Oligonucleotides
title_short Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
title_full Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
title_fullStr Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
title_full_unstemmed Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
title_sort Variable-temperature size exclusion chromatography for the study of the structural changes in G-quadruplex
dc.creator.none.fl_str_mv Benabou Zdaou, Sanae
Eritja i Casadellà, Ramon
Gargallo Gómez, Raimundo
author Benabou Zdaou, Sanae
author_facet Benabou Zdaou, Sanae
Eritja i Casadellà, Ramon
Gargallo Gómez, Raimundo
author_role author
author2 Eritja i Casadellà, Ramon
Gargallo Gómez, Raimundo
author2_role author
author
dc.subject.none.fl_str_mv Àcids nucleics
G-estructures
Oligonucleòtids
Nucleic acids
G-structures
Oligonucleotides
topic Àcids nucleics
G-estructures
Oligonucleòtids
Nucleic acids
G-structures
Oligonucleotides
description The conformational equilibria of a guanine-rich sequence found at the promoter region of the human c-kit oncogene are studied by means of circular dichroism spectroscopy (CD) and variable-temperature Size Exclusion Chromatography (SEC). It is shown that the wild sequence ckit21 exists as a mixture of monomeric and multimeric G-quadruplex. Appropriate mutation of several bases in the wild sequence produces the shift from parallel to antiparallel G-quadruplex, as well as the disappearance of multimeric species. The shift from the antiparallel to the parallel conformation induced by temperature is reflected in both CD and SEC profiles.
publishDate 2013
dc.date.none.fl_str_mv 2013
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/151262
url https://hdl.handle.net/2445/151262
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1155/2013/631875
ISRN Biochemistry, 2013, vol. 2013, p. 1-7
https://doi.org/10.1155/2013/631875
dc.rights.none.fl_str_mv cc-by (c) Benabou Zdaou, Sanae et al., 2013
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Benabou Zdaou, Sanae et al., 2013
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7 p.
application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869416485841010688
score 15,81155

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