Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading

Omega-3 (ω3 or n-3) long-chain polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to be originated almost exclusively from aquatic (mostly marine) single-cell...

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Autores: Kabeya, Naoki, Gür, İbrahim, Oboh, Angela, Ove Evjemo, Jan, Malzahn, Arne M., Hontoria, Francisco, Navarro, Juan Carlos, Monroig, Óscar
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/218918
Acceso en línea:http://hdl.handle.net/10261/218918
Access Level:acceso abierto
Palabra clave:Biosynthesis
Methyl-end desaturase
N-3 long-chain PUFA
Polychaetes
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spelling Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgradingKabeya, NaokiGür, İbrahimOboh, AngelaOve Evjemo, JanMalzahn, Arne M.Hontoria, FranciscoNavarro, Juan CarlosMonroig, ÓscarBiosynthesisMethyl-end desaturaseN-3 long-chain PUFAPolychaetesOmega-3 (ω3 or n-3) long-chain polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to be originated almost exclusively from aquatic (mostly marine) single-cell organisms. Yet, a recent study has discovered that many invertebrates possess a type of enzymes called methyl-end desaturases (ωx) that enables them to endogenously produce n-3 long-chain PUFA and could make a significant contribution to production of these compounds in the marine environment. Polychaetes are major components of benthic fauna and thus important to maintain a robust food web as a recycler of organic matter and a prey item for higher trophic level species like fish. In the present study, we investigated the ωx enzymes from the common ragworm Hediste diversicolor, a common inhabitant in sedimentary littoral ecosystems of the North Atlantic. Functional assays of the H. diversicolor ωx demonstrated unique desaturation capacities. An ω3 desaturase mediated the conversion of n-6 fatty acid substrates into their corresponding n-3 products including DHA. A further enzyme possessed unique regioselectivities combining both ω6 and ω3 desaturase activities. These results illustrate that the long-chain PUFA biosynthetic enzymatic machinery of aquatic invertebrates such as polychaetes is highly diverse and clarify that invertebrates can be major contributors to fatty acid trophic upgrading in aquatic food webs.This study was funded through the project IMPROMEGA of the Ministry of Science, Innovation and Universities, Spanish Government (grant no. RTI2018-095119-B-I00, MCIU/AEI/FEDER, UE). N.K. was funded by the Japan Society for the Promotion of Science through Grant-in-Aid for JSPS Fellows (grant no. 16J06812). İ.G. received funding from the Ministry of National Education, Republic of Turkey. A.O. was funded by the Commonwealth Scholarship Commission (grant no. NGCS-2014-438) in the UK. A.M.M. acknowledges the Norwegian Research Council project POLYCHAETE (grant no. 280836) for funding.Peer reviewedRoyal Society (Great Britain)Ministerio de Ciencia e Innovación (España)European CommissionJapan Society for the Promotion of ScienceCommonwealth Scholarship CommissionNorwegian Research CouncilAgencia Estatal de Investigación (España)Hontoria, Francisco [0000-0003-2466-1375]Navarro, Juan Carlos [0000-0001-6976-6686]Monroig, Óscar [0000-0001-8712-0440]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/218918reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Español#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095119-B-I00RTI2018-095119-B-I00/AEI/10.13039/501100011033http://dx.doi.org/10.1098/rstb.2019.0654Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2189182026-05-22T06:33:51Z
dc.title.none.fl_str_mv Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
title Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
spellingShingle Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
Kabeya, Naoki
Biosynthesis
Methyl-end desaturase
N-3 long-chain PUFA
Polychaetes
title_short Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
title_full Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
title_fullStr Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
title_full_unstemmed Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
title_sort Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
dc.creator.none.fl_str_mv Kabeya, Naoki
Gür, İbrahim
Oboh, Angela
Ove Evjemo, Jan
Malzahn, Arne M.
Hontoria, Francisco
Navarro, Juan Carlos
Monroig, Óscar
author Kabeya, Naoki
author_facet Kabeya, Naoki
Gür, İbrahim
Oboh, Angela
Ove Evjemo, Jan
Malzahn, Arne M.
Hontoria, Francisco
Navarro, Juan Carlos
Monroig, Óscar
author_role author
author2 Gür, İbrahim
Oboh, Angela
Ove Evjemo, Jan
Malzahn, Arne M.
Hontoria, Francisco
Navarro, Juan Carlos
Monroig, Óscar
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Japan Society for the Promotion of Science
Commonwealth Scholarship Commission
Norwegian Research Council
Agencia Estatal de Investigación (España)
Hontoria, Francisco [0000-0003-2466-1375]
Navarro, Juan Carlos [0000-0001-6976-6686]
Monroig, Óscar [0000-0001-8712-0440]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biosynthesis
Methyl-end desaturase
N-3 long-chain PUFA
Polychaetes
topic Biosynthesis
Methyl-end desaturase
N-3 long-chain PUFA
Polychaetes
description Omega-3 (ω3 or n-3) long-chain polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to be originated almost exclusively from aquatic (mostly marine) single-cell organisms. Yet, a recent study has discovered that many invertebrates possess a type of enzymes called methyl-end desaturases (ωx) that enables them to endogenously produce n-3 long-chain PUFA and could make a significant contribution to production of these compounds in the marine environment. Polychaetes are major components of benthic fauna and thus important to maintain a robust food web as a recycler of organic matter and a prey item for higher trophic level species like fish. In the present study, we investigated the ωx enzymes from the common ragworm Hediste diversicolor, a common inhabitant in sedimentary littoral ecosystems of the North Atlantic. Functional assays of the H. diversicolor ωx demonstrated unique desaturation capacities. An ω3 desaturase mediated the conversion of n-6 fatty acid substrates into their corresponding n-3 products including DHA. A further enzyme possessed unique regioselectivities combining both ω6 and ω3 desaturase activities. These results illustrate that the long-chain PUFA biosynthetic enzymatic machinery of aquatic invertebrates such as polychaetes is highly diverse and clarify that invertebrates can be major contributors to fatty acid trophic upgrading in aquatic food webs.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/218918
url http://hdl.handle.net/10261/218918
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095119-B-I00
RTI2018-095119-B-I00/AEI/10.13039/501100011033
http://dx.doi.org/10.1098/rstb.2019.0654
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society (Great Britain)
publisher.none.fl_str_mv Royal Society (Great Britain)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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