Impact of CSF storage volume on the analysis of Alzheimer's disease biomarkers on an automated platform

Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10...

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Bibliographic Details
Authors: Delaby, C, Munoz, L, Torres, S, Nadal, A, Le Bastard, N, Lehmann, S, Lleo, A, Alcolea, D
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repository:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p2966
Online Access:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=2966
https://hal.umontpellier.fr/hal-02626119
Access Level:Open access
Keyword:Cerebrospinal fluid
Biomarkers
Amyloid
Tau
Lumipulse
Alzheimer's disease
Description
Summary:Objectives: To assess the potential influence of the ratio between the storage tube surface area and the volume of cerebrospinal fluid (CSF) (surface/volume) on the quantifications of four Alzheimer's disease (AD) biomarkers on the Lumipulse G600II automated platform. Methods: CSF samples of 10 consecutive patients were stored in 2 ml polypropylene tubes containing four different CSF volumes: 1.5 ml, 1 ml, 0.5 ml and 0.25 ml. Concentration of CSF A beta 1-42, A beta 1-40, t-Tau and p-Tau were measured in all aliquots using the LUMIPULSE G600II automated platform from Fujirebio. Results: Levels of CSF A beta 1-42 and A beta 1-40 were lower in samples stored with lower volumes (higher surface/volume ratios). This decrease was partly compensated by using the ratio A beta 1-42/A beta 1-40. Quantification of t-Tau and p-Tau were not influenced by this pre-analytical condition. Conclusion: The surface/volume ratio can potentially influence the results of amyloid AD biomarkers. It appears essential to take into account the surface/volume ratio of the storage tubes when quantifying CSF biomarkers in clinical routine.

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