Importance of cerebrospinal fluid storage conditions for the Alzheimer's disease diagnostics on an automated platform
Objectives Alzheimer's disease (AD) is considered the most common cause of dementia in older people. Cerebrospinal fluid (CSF) A beta 1-42, A beta 1-40, total Tau (t-Tau), and phospho Tau (p-Tau) are important biomarkers for the diagnosis, however, they are highly dependent on the pre-analytica...
| Autores: | , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
| Repositorio: | r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| OAI Identifier: | oai:iibsantpau.fundanetsuite.com:p8470 |
| Acceso en línea: | https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=8470 https://ddd.uab.cat/record/271555 |
| Access Level: | acceso abierto |
| Palabra clave: | Alzheimer's disease amyloid automated platforms biomarkers cerebrospinal fluid Tau |
| Sumario: | Objectives Alzheimer's disease (AD) is considered the most common cause of dementia in older people. Cerebrospinal fluid (CSF) A beta 1-42, A beta 1-40, total Tau (t-Tau), and phospho Tau (p-Tau) are important biomarkers for the diagnosis, however, they are highly dependent on the pre-analytical conditions. Our aim was to investigate the potential influence of different storage conditions on the simultaneous quantification of these biomarkers in a fully-automated platform to accommodate easier pre-analytical conditions for laboratories. Methods CSF samples were obtained from 11 consecutive patients. A beta 1-42, A beta 1-40, p-Tau, and t-Tau were quantified using the LUMIPULSE G600II automated platform. Results Temperature and storage days significantly influenced A beta 1-42 and A beta 1-40 with concentrations decreasing with days spent at 4 degrees C. The use of the A beta 1-42/A beta 1-40 ratio could partly compensate it. P-Tau and t-Tau were not affected by any of the tested storage conditions. For conditions involving storage at 4 degrees C, a correction factor of 1.081 can be applied. Diagnostic agreement was almost perfect in all conditions. Conclusions Cutoffs calculated in samples stored at -80 degrees C can be safely used in samples stored at -20 degrees C for 15-16 days or up to two days at RT and subsequent freezing at -80 degrees C. For samples stored at 4 degrees C, cutoffs would require applying a correction factor, allowing to work with the certainty of reaching the same clinical diagnosis. |
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