Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix

Mycotoxins are toxic compounds for humans and animals that are produced by fungi. Mycotoxin contamination in feed is a global safety concern and effective control of these compounds in this matrix is needed. This study proposes a simple, cost-effective analytical method based on liquid chromatograph...

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Autores: Muñoz-Solano, B. (Borja)|||/items/a1333408-1dae-4822-8868-f7ccab1743f5, Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/60081
Acceso en línea:https://hdl.handle.net/10171/60081
Access Level:acceso abierto
Palabra clave:Feed
Mycotoxins
Liquid chromatography
Fluorescence
Multidetection
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spelling Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrixMuñoz-Solano, B. (Borja)|||/items/a1333408-1dae-4822-8868-f7ccab1743f5Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833fFeedMycotoxinsLiquid chromatographyFluorescenceMultidetectionMycotoxins are toxic compounds for humans and animals that are produced by fungi. Mycotoxin contamination in feed is a global safety concern and effective control of these compounds in this matrix is needed. This study proposes a simple, cost-effective analytical method based on liquid chromatography coupled with a fluorescence detector, which is suitable for the routine monitoring of some of the most important mycotoxins in feed: aflatoxins (G2, G1, B2, and B1), zearalenone, and ochratoxins A and B. Mycotoxin extraction, chromatographic separation and quantification are carried out simultaneously for all mycotoxins. The extraction procedure is performed using acetonitrile, water and orthophosphoric acid (80:19:1). Purification of the extract is carried out using an OASIS PRIME HLB solid-phase extraction cartridge followed by a dispersive liquid–liquid microextraction procedure. Aflatoxins G1 and B1 are derivatized post-column (photochemical reactor at 254 nm) to increase their signal. The method has been validated in feed for pigs, cows, sheep, and poultry with very satisfactory results. The detection limits are 2 μg/kg for aflatoxins B1 and G1, 0.64 μg/kg for aflatoxins B2 and G2, 42 μg/kg for zearalenone, and 5 μg/kg for ochratoxins A and B. These values are low enough to allow for monitoring of these mycotoxins in feed. Global recovery values were between 73.6% and 88.0% for all toxins with a relative standard deviation (RSD) % < 7%. This methodology will facilitate laboratory control and analysis of mycotoxins in feed.MDPIDadun. Depósito Académico Digital Universidad de Navarra20212021-02-1120202020-01-0120202020-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/60081reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/600812026-06-21T12:47:57Z
dc.title.none.fl_str_mv Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
title Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
spellingShingle Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
Muñoz-Solano, B. (Borja)|||/items/a1333408-1dae-4822-8868-f7ccab1743f5
Feed
Mycotoxins
Liquid chromatography
Fluorescence
Multidetection
title_short Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
title_full Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
title_fullStr Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
title_full_unstemmed Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
title_sort Mycotoxin determination in animal feed: an LC-FLD method for simultaneous quantification of aflatoxins, ochratoxins and zearelanone in this matrix
dc.creator.none.fl_str_mv Muñoz-Solano, B. (Borja)|||/items/a1333408-1dae-4822-8868-f7ccab1743f5
Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
author Muñoz-Solano, B. (Borja)|||/items/a1333408-1dae-4822-8868-f7ccab1743f5
author_facet Muñoz-Solano, B. (Borja)|||/items/a1333408-1dae-4822-8868-f7ccab1743f5
Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
author_role author
author2 Gonzalez-Peñas, E. (Elena)|||/items/0e4b9ed7-4224-4664-a4f1-c6403a8c833f
author2_role author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Feed
Mycotoxins
Liquid chromatography
Fluorescence
Multidetection
topic Feed
Mycotoxins
Liquid chromatography
Fluorescence
Multidetection
description Mycotoxins are toxic compounds for humans and animals that are produced by fungi. Mycotoxin contamination in feed is a global safety concern and effective control of these compounds in this matrix is needed. This study proposes a simple, cost-effective analytical method based on liquid chromatography coupled with a fluorescence detector, which is suitable for the routine monitoring of some of the most important mycotoxins in feed: aflatoxins (G2, G1, B2, and B1), zearalenone, and ochratoxins A and B. Mycotoxin extraction, chromatographic separation and quantification are carried out simultaneously for all mycotoxins. The extraction procedure is performed using acetonitrile, water and orthophosphoric acid (80:19:1). Purification of the extract is carried out using an OASIS PRIME HLB solid-phase extraction cartridge followed by a dispersive liquid–liquid microextraction procedure. Aflatoxins G1 and B1 are derivatized post-column (photochemical reactor at 254 nm) to increase their signal. The method has been validated in feed for pigs, cows, sheep, and poultry with very satisfactory results. The detection limits are 2 μg/kg for aflatoxins B1 and G1, 0.64 μg/kg for aflatoxins B2 and G2, 42 μg/kg for zearalenone, and 5 μg/kg for ochratoxins A and B. These values are low enough to allow for monitoring of these mycotoxins in feed. Global recovery values were between 73.6% and 88.0% for all toxins with a relative standard deviation (RSD) % < 7%. This methodology will facilitate laboratory control and analysis of mycotoxins in feed.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01
2020
2020-01-01
2021
2021-02-11
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/60081
url https://hdl.handle.net/10171/60081
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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