Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region

OBJECTIVES: To examine the relationship between antibody status and cycle threshold (Ct) values, the prognostic value of the latter for COVID-19 patients, and the inter-assay comparability of SARS-CoV-2 Ct values. METHODS: In 347 COVID-19 inpatients, SARS-CoV-2 Ct values (via reverse transcription-q...

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Autores: Markewitz, Robert, Torge, Antje, Wandinger, Klaus-Peter, Pauli, Daniela, Dargvainiene, Justina, Franke, Andre, Bujanda Fernández de Pierola, Luis, Marimón Ortiz de Zarate, José María, Bañales Asurmendi, Jesús María, Gutiérrez Stampa, María Angeles, Nafría, Beatriz, Junker, Ralf
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/53138
Acceso en línea:http://hdl.handle.net/10810/53138
Access Level:acceso abierto
Palabra clave:COVID-19
Ct value
RT-qPCR
SARS-CoV-2
anti-SARS-CoV-2 antibodies
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spelling Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence regionMarkewitz, RobertTorge, AntjeWandinger, Klaus-PeterPauli, DanielaDargvainiene, JustinaFranke, AndreBujanda Fernández de Pierola, LuisMarimón Ortiz de Zarate, José MaríaBañales Asurmendi, Jesús MaríaGutiérrez Stampa, María AngelesNafría, BeatrizJunker, RalfCOVID-19Ct valueRT-qPCRSARS-CoV-2anti-SARS-CoV-2 antibodiesOBJECTIVES: To examine the relationship between antibody status and cycle threshold (Ct) values, the prognostic value of the latter for COVID-19 patients, and the inter-assay comparability of SARS-CoV-2 Ct values. METHODS: In 347 COVID-19 inpatients, SARS-CoV-2 Ct values (via reverse transcription-quantitative polymerase chain reaction) on admission were compared between 2 assays and correlated with the antibody response (in the course of the disease), the clinical course and the time since onset of symptoms. RESULTS: Ct values for 2 of 3 target genes showed significant differences between the 2 assays used (P=0.012 and P<0.0001). Ct values were significantly higher for antibody positive patients (P<0.0001) and positively correlated with the amount of time since onset of symptoms (R: 0.332-0.363; P<0.001). Patients with fatal outcomes showed higher viral loads than survivors (P<0.0001). CONCLUSIONS: Ct values depend strongly on assay used and target gene examined and should not be used as quantitative values to guide therapeutic or diagnostic decisions. The inverse association between antibody status and viral load suggests that antibodies contribute to the elimination of the virus, independent of the outcome, which is influenced by the viral load on admission and might depend more strongly on other parts of the immune response.Elsevier202120212021info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/53138reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.sciencedirect.com/science/article/pii/S1201971221005701?via%3Dihubinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/2021 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/by-nc-nd/4.0/ )Atribución-NoComercial-SinDerivadas 3.0 Españaoai:addi.ehu.eus:10810/531382026-06-18T09:23:17Z
dc.title.none.fl_str_mv Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
title Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
spellingShingle Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
Markewitz, Robert
COVID-19
Ct value
RT-qPCR
SARS-CoV-2
anti-SARS-CoV-2 antibodies
title_short Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
title_full Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
title_fullStr Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
title_full_unstemmed Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
title_sort Analysis of SARS-CoV-2 reverse transcription-quantitative polymerase chain reaction cycle threshold values vis-à-vis anti-SARS-CoV-2 antibodies from a high incidence region
dc.creator.none.fl_str_mv Markewitz, Robert
Torge, Antje
Wandinger, Klaus-Peter
Pauli, Daniela
Dargvainiene, Justina
Franke, Andre
Bujanda Fernández de Pierola, Luis
Marimón Ortiz de Zarate, José María
Bañales Asurmendi, Jesús María
Gutiérrez Stampa, María Angeles
Nafría, Beatriz
Junker, Ralf
author Markewitz, Robert
author_facet Markewitz, Robert
Torge, Antje
Wandinger, Klaus-Peter
Pauli, Daniela
Dargvainiene, Justina
Franke, Andre
Bujanda Fernández de Pierola, Luis
Marimón Ortiz de Zarate, José María
Bañales Asurmendi, Jesús María
Gutiérrez Stampa, María Angeles
Nafría, Beatriz
Junker, Ralf
author_role author
author2 Torge, Antje
Wandinger, Klaus-Peter
Pauli, Daniela
Dargvainiene, Justina
Franke, Andre
Bujanda Fernández de Pierola, Luis
Marimón Ortiz de Zarate, José María
Bañales Asurmendi, Jesús María
Gutiérrez Stampa, María Angeles
Nafría, Beatriz
Junker, Ralf
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv COVID-19
Ct value
RT-qPCR
SARS-CoV-2
anti-SARS-CoV-2 antibodies
topic COVID-19
Ct value
RT-qPCR
SARS-CoV-2
anti-SARS-CoV-2 antibodies
description OBJECTIVES: To examine the relationship between antibody status and cycle threshold (Ct) values, the prognostic value of the latter for COVID-19 patients, and the inter-assay comparability of SARS-CoV-2 Ct values. METHODS: In 347 COVID-19 inpatients, SARS-CoV-2 Ct values (via reverse transcription-quantitative polymerase chain reaction) on admission were compared between 2 assays and correlated with the antibody response (in the course of the disease), the clinical course and the time since onset of symptoms. RESULTS: Ct values for 2 of 3 target genes showed significant differences between the 2 assays used (P=0.012 and P<0.0001). Ct values were significantly higher for antibody positive patients (P<0.0001) and positively correlated with the amount of time since onset of symptoms (R: 0.332-0.363; P<0.001). Patients with fatal outcomes showed higher viral loads than survivors (P<0.0001). CONCLUSIONS: Ct values depend strongly on assay used and target gene examined and should not be used as quantitative values to guide therapeutic or diagnostic decisions. The inverse association between antibody status and viral load suggests that antibodies contribute to the elimination of the virus, independent of the outcome, which is influenced by the viral load on admission and might depend more strongly on other parts of the immune response.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/53138
url http://hdl.handle.net/10810/53138
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1201971221005701?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Atribución-NoComercial-SinDerivadas 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Atribución-NoComercial-SinDerivadas 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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