Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates

The design of synthetic bone grafts to foster bone formation is a challenge in regenerative medicine. Understanding the interaction of bone substitutes with osteoclasts is essential, since osteoclasts not only drive a timely resorption of the biomaterial, but also trigger osteoblast activity. In thi...

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Autores: Ciapetti G, di Pompo G, Avnet S, Martini D, Diez-Escudero A, Montufar EB, Ginebra MP, Baldini N
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:fsjd.fundanetsuite.com:p10591
Acesso em linha:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10591
http://hdl.handle.net/2117/104354
Access Level:acceso abierto
Palavra-chave:Bone resorption, Differentiation, Hydroxyapatite, Ionic exchange, Osteoclasts, Topography
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spelling Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substratesCiapetti Gdi Pompo GAvnet SMartini DDiez-Escudero AMontufar EBGinebra MPBaldini NBone resorption, Differentiation, Hydroxyapatite, Ionic exchange, Osteoclasts, TopographyThe design of synthetic bone grafts to foster bone formation is a challenge in regenerative medicine. Understanding the interaction of bone substitutes with osteoclasts is essential, since osteoclasts not only drive a timely resorption of the biomaterial, but also trigger osteoblast activity. In this study, the adhesion and differentiation of human blood-derived osteoclast precursors (OCP) on two different micro-nanostructured biomimetic hydroxyapatite materials consisting in coarse (HA-C) and fine HA (HA-F) crystals, in comparison with sintered stoichiometric HA (sin-HA, reference material), were investigated. Osteoclasts were induced to differentiate by RANKL-containing supernatant using cell/substrate direct and indirect contact systems, and calcium (Ca++) and phosphorus (P5+) in culture medium were measured. We observed that OCP adhered to the experimental surfaces, and that osteoclast-like cells formed at a rate influenced by the micro- and nano-structure of HA, which also modulate extracellular Ca++. Qualitative differences were found between OCP on biomimetic HA-C and HA-F and their counterparts on plastic and sin-HA. On HA-C and HA-F cells shared typical features of mature osteoclasts, i.e. podosomes, multinuclearity, tartrate acid phosphatase (TRAP)-positive staining, and TRAP5b-enzyme release. However, cells were less in number compared to those on plastic or on sin-HA, and they did not express some specific osteoclast markers. In conclusion, blood-derived OCP are able to attach to biomimetic and sintered HA substrates, but their subsequent fusion and resorptive activity are hampered by surface micro-nano-structure. Indirect cultures suggest that fusion of OCP is sensitive to topography and to extracellular calcium.ELSEVIER SCI LTD2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10591http://hdl.handle.net/2117/104354Acta BiomaterialiaISSN: 17427061ISSNe: 18787568reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:fsjd.fundanetsuite.com:p105912026-05-27T12:37:41Z
dc.title.none.fl_str_mv Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
title Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
spellingShingle Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
Ciapetti G
Bone resorption, Differentiation, Hydroxyapatite, Ionic exchange, Osteoclasts, Topography
title_short Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
title_full Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
title_fullStr Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
title_full_unstemmed Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
title_sort Osteoclast differentiation from human blood precursors on biomimetic calcium-phosphate substrates
dc.creator.none.fl_str_mv Ciapetti G
di Pompo G
Avnet S
Martini D
Diez-Escudero A
Montufar EB
Ginebra MP
Baldini N
author Ciapetti G
author_facet Ciapetti G
di Pompo G
Avnet S
Martini D
Diez-Escudero A
Montufar EB
Ginebra MP
Baldini N
author_role author
author2 di Pompo G
Avnet S
Martini D
Diez-Escudero A
Montufar EB
Ginebra MP
Baldini N
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bone resorption, Differentiation, Hydroxyapatite, Ionic exchange, Osteoclasts, Topography
topic Bone resorption, Differentiation, Hydroxyapatite, Ionic exchange, Osteoclasts, Topography
description The design of synthetic bone grafts to foster bone formation is a challenge in regenerative medicine. Understanding the interaction of bone substitutes with osteoclasts is essential, since osteoclasts not only drive a timely resorption of the biomaterial, but also trigger osteoblast activity. In this study, the adhesion and differentiation of human blood-derived osteoclast precursors (OCP) on two different micro-nanostructured biomimetic hydroxyapatite materials consisting in coarse (HA-C) and fine HA (HA-F) crystals, in comparison with sintered stoichiometric HA (sin-HA, reference material), were investigated. Osteoclasts were induced to differentiate by RANKL-containing supernatant using cell/substrate direct and indirect contact systems, and calcium (Ca++) and phosphorus (P5+) in culture medium were measured. We observed that OCP adhered to the experimental surfaces, and that osteoclast-like cells formed at a rate influenced by the micro- and nano-structure of HA, which also modulate extracellular Ca++. Qualitative differences were found between OCP on biomimetic HA-C and HA-F and their counterparts on plastic and sin-HA. On HA-C and HA-F cells shared typical features of mature osteoclasts, i.e. podosomes, multinuclearity, tartrate acid phosphatase (TRAP)-positive staining, and TRAP5b-enzyme release. However, cells were less in number compared to those on plastic or on sin-HA, and they did not express some specific osteoclast markers. In conclusion, blood-derived OCP are able to attach to biomimetic and sintered HA substrates, but their subsequent fusion and resorptive activity are hampered by surface micro-nano-structure. Indirect cultures suggest that fusion of OCP is sensitive to topography and to extracellular calcium.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10591
http://hdl.handle.net/2117/104354
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=10591
http://hdl.handle.net/2117/104354
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER SCI LTD
publisher.none.fl_str_mv ELSEVIER SCI LTD
dc.source.none.fl_str_mv Acta Biomaterialia
ISSN: 17427061
ISSNe: 18787568
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
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