Effect of the extract and constituents from Hancornia speciosa fruits in osteoclasts
Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential...
| Autores: | , , , , , , , , , , , |
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| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2019 |
| País: | Brasil |
| Recursos: | Universidade Federal de Minas Gerais (UFMG) |
| Repositorio: | Repositório Institucional da UFMG |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.ufmg.br:1843/81917 |
| Acesso em linha: | https://doi.org/10.1055/a-0829-0454 http://hdl.handle.net/1843/81917 https://orcid.org/0000-0002-6642-3650 https://orcid.org/0000-0001-9468-376X https://orcid.org/0000-0001-9623-7835 https://orcid.org/0009-0001-2072-5282 https://orcid.org/0000-0003-2437-5707 https://orcid.org/0000-0002-9939-6900 https://orcid.org/0000-0002-4044-9932 https://orcid.org/0000-0002-1770-9978 https://orcid.org/0000-0002-6589-4784 https://orcid.org/0000-0002-0986-0184 https://orcid.org/0000-0002-7910-8975 |
| Access Level: | acceso abierto |
| Palavra-chave: | Hancornia speciosa Apocynaceae Cell culture Osteoclasts Bone resorption Cell culture techniques Osteogenesis |
| Resumo: | Hancornia speciosa is a medicinal species traditionally used in Brazilian folk medicine to treat a variety of conditions. Compounds isolated from the leaves, bark, and trunk of this plant have shown therapeutic properties, but only recently have the fruits of H. speciosa been explored for potential pharmacological applications. The present study investigated the effects of an ethanolic extract from the fruits, fractions, and compounds thereof in bone resorbing cells. Primary osteoclast cultures from bone marrow cells and osteoclasts derived from a monocyte/macrophage cell line, RAW 264.7, were incubated with different concentrations of the ethanolic extract, ethyl acetate fraction, water fraction, quinic acid, and L-(+)-bornesitol. In RAW 264.7 cell cultures, quinic acid significantly reduced osteoclast formation. In bone marrow cell-derived osteoclasts, the ethyl acetate fraction induced a decrease in the number of osteoclasts, promoting a remarkable reduction in the mean area of those cells and in their resorption activity. The compounds quinic acid and bornesitol also affected bone marrow cell-derived osteoclasts. In both cell cultures, the substances tested did not affect cell viability/proliferation. In conclusion, components extracted from H. speciosa fruit affected the cells responsible for bone resorption, making them promising tools for interference in osteoclastogenesis. |
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