UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium

In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein...

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Autores: Palma Dovis, Leopoldo, Ruiz de Escudero Fuentemilla, Íñigo, Mañeru Oria, Francisco Javier, Berry, Colin, Caballero Murillo, Primitivo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/52301
Acceso en línea:https://hdl.handle.net/2454/52301
Access Level:acceso abierto
Palabra clave:Bacillus megaterium
Bacillus thuringiensis
Bioinsecticide formulations
Biological control
Insect pests
Microencapsulation
Priestia megaterium
Vip3 proteins
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spelling UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megateriumPalma Dovis, LeopoldoRuiz de Escudero Fuentemilla, ÍñigoMañeru Oria, Francisco JavierBerry, ColinCaballero Murillo, PrimitivoBacillus megateriumBacillus thuringiensisBioinsecticide formulationsBiological controlInsect pestsMicroencapsulationPriestia megateriumVip3 proteinsIn this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light.This research was funded by the Spanish Ministry of Science and Innovation grant number AGL2009-13340-C02 and by the Universidad Pública de Navarra. Leopoldo Palma would like to express gratitude to the Spanish Government-Universities Ministry, the NextGenerationEU and Recovery, Transformation, and Resilience plans for funding his awarded María Zambrano contract (ref. ZA21-003).ElsevierInstitute for Multidisciplinary Research in Applied Biology - IMAB2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/52301reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MICINN//AGL2009-13340-C02-02© 2024 The Author(s). This is an open access article under the CC BY license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/523012026-06-17T12:41:47Z
dc.title.none.fl_str_mv UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
title UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
spellingShingle UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
Palma Dovis, Leopoldo
Bacillus megaterium
Bacillus thuringiensis
Bioinsecticide formulations
Biological control
Insect pests
Microencapsulation
Priestia megaterium
Vip3 proteins
title_short UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_full UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_fullStr UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_full_unstemmed UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
title_sort UV protection and insecticidal activity of microencapsulated Vip3Ag4 protein in Bacillus megaterium
dc.creator.none.fl_str_mv Palma Dovis, Leopoldo
Ruiz de Escudero Fuentemilla, Íñigo
Mañeru Oria, Francisco Javier
Berry, Colin
Caballero Murillo, Primitivo
author Palma Dovis, Leopoldo
author_facet Palma Dovis, Leopoldo
Ruiz de Escudero Fuentemilla, Íñigo
Mañeru Oria, Francisco Javier
Berry, Colin
Caballero Murillo, Primitivo
author_role author
author2 Ruiz de Escudero Fuentemilla, Íñigo
Mañeru Oria, Francisco Javier
Berry, Colin
Caballero Murillo, Primitivo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Institute for Multidisciplinary Research in Applied Biology - IMAB
dc.subject.none.fl_str_mv Bacillus megaterium
Bacillus thuringiensis
Bioinsecticide formulations
Biological control
Insect pests
Microencapsulation
Priestia megaterium
Vip3 proteins
topic Bacillus megaterium
Bacillus thuringiensis
Bioinsecticide formulations
Biological control
Insect pests
Microencapsulation
Priestia megaterium
Vip3 proteins
description In this study, secretable Vip3Ag4 protein was encapsulated in Bacillus megaterium and used for quantitative bioassays, in order to determine the UV photoprotective capacity of the cell, for preventing inactivation of the insecticidal activity of the protein. The non-encapsulated and purified protein was exposed to the UV light showing a LC50 of 518 ng/cm2 against Spodoptera littoralis larvae, whereas the exposed encapsulated protein exhibited 479 ng/cm2. In addition to the capability to accumulate Vip3 proteins for the development of novel insecticidal formulates, the B. megaterium cell has demonstrated to provide moderate protection against the deleterious action of UV light.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/52301
url https://hdl.handle.net/2454/52301
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2009-13340-C02-02
dc.rights.none.fl_str_mv © 2024 The Author(s). This is an open access article under the CC BY license.
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2024 The Author(s). This is an open access article under the CC BY license.
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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