Carbon nanodots modified-electrode for peroxide-free cholesterol biosensing and biofuel cell design
[EN] The determination of cholesterol is greatly important because high concentrations of this biomarker are associated to heart disease. Moreover, cholesterol can be used as a fuel in enzymatic fuel cells operating under physiological conditions. Here, we present a cholesterol biosensor and a perox...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/343993 |
| Acceso en línea: | http://hdl.handle.net/10261/343993 |
| Access Level: | acceso abierto |
| Palabra clave: | Carbon nanodots Dehydrogenase Cholesterol Biosensor Biofuel cell |
| Sumario: | [EN] The determination of cholesterol is greatly important because high concentrations of this biomarker are associated to heart disease. Moreover, cholesterol can be used as a fuel in enzymatic fuel cells operating under physiological conditions. Here, we present a cholesterol biosensor and a peroxide-free biofuel cell based on the electrocatalytic oxidation of the NADH generated during the enzymatic reaction of cholesterol dehydrogenase (ChDH) as an alternative to the HO biosensing strategies used with cholesterol oxidase-bioelectrodes. Azure A functionalized-carbon nanodots were used as NADH oxidation electrocatalysts and for ChDH covalent immobilization. The biosensor responded linearly to cholesterol concentrations up to 1.7 mM with good sensitivity (4.50 mA cm M) and at a low potential. The ChDH bioelectrode was combined with an O-reducing bilirubin oxidase cathode to produce electrical energy using cholesterol as fuel and O as oxidant. Furthermore, the resulting enzymatic fuel cell was tested in human serum naturally containing free cholesterol. |
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