Total data_BA

Methods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic b...

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Bibliographic Details
Author: Casado, Marta
Format: conjunto de datos
Publication Date:2022
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/280249
Online Access:http://hdl.handle.net/10261/280249
https://doi.org/10.20350/digitalCSIC/14755
Access Level:Open access
Keyword:COX-2
PGE2
BDL
Liver Bile Acids
Cholestasis
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spelling Total data_BACasado, MartaCOX-2PGE2BDLLiver Bile AcidsCholestasisMethods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic beads (Precellys). For each 50 mg of tissue, 300 μl of methanol and the deuterated internal standards were added and tissues were homogenized in a Precellys 24 Dual system equipped with a Criolys cooler (Precellys). Samples were analyzed using an Acquity UPLC system (Waters, UK) equipped with an Acquity UPLC BEH C18 column (1.7μm, 2.1 x 100 mm; Waters). The MS analysis was performed using a Waters Xevo TQ-XS mass spectrometer (Waters) with an ESI source working in the negative-ion mode.Cyclooxygenase-2 (COX-2) is involved in different liver diseases, but little is known about the significance of COX-2 or its metabolites in cholestatic injury. This study was designed to elucidate the role of COX-2 expression during the pathogenesis of cholestasis. Thus, we investigated the mechanisms underlying the role of COX-2 and its derived prostaglandins in modulating cell survival, inflammation, oxidative stress status and the synthesis and excretion of bile acids (BA) in response to cholestatic liver injury. We used genetically modified mice constitutively expressing human COX-2 (hCOX-2-Tg) specifically in hepatocytes. Transgenic mice (hCOX-2-Tg) and their wild-type (Wt) littermates were either subjected to a common bile duct ligation (BDL) to establish an experimental model of obstructive cholestasis. We performed an exhaustive analysis of the different types of bile acids (total, primary, secondary, conjugated, non-conjugated and hydrophilic, α-, β- and ω-muricholic acid) in plasma and in liver tissue from Wt and h-COX-2 Tg mice. Samples were analyzed at Instituto de Investigación Sanitaria La Fe (Valencia, Spain) detecting a total of 31 analytes.Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación 10.13039/501100011033 (PID2019-108977RB-I00)NoDIGITAL.CSICMinisterio de Ciencia e Innovación (España)Casado, Marta [0000-0001-6457-4650]Casado, Marta [mcasado@ibv.csic.es]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1application/vnd.ms-excelhttp://hdl.handle.net/10261/280249https://doi.org/10.20350/digitalCSIC/14755reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108977RB-I00MicrosoftSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2802492026-05-22T06:33:51Z
dc.title.none.fl_str_mv Total data_BA
title Total data_BA
spellingShingle Total data_BA
Casado, Marta
COX-2
PGE2
BDL
Liver Bile Acids
Cholestasis
title_short Total data_BA
title_full Total data_BA
title_fullStr Total data_BA
title_full_unstemmed Total data_BA
title_sort Total data_BA
dc.creator.none.fl_str_mv Casado, Marta
author Casado, Marta
author_facet Casado, Marta
author_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Casado, Marta [0000-0001-6457-4650]
Casado, Marta [mcasado@ibv.csic.es]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv COX-2
PGE2
BDL
Liver Bile Acids
Cholestasis
topic COX-2
PGE2
BDL
Liver Bile Acids
Cholestasis
description Methods 20 μL of plasma samples were spiked with deuterated internal standards stock solution. Then proteins were precipitated and supernatants were dried and reconstituted in methanol:water (50:50, V/V). Besides, approximately 50 mg of each tissue were placed in 2 ml tubes containing CK14 ceramic beads (Precellys). For each 50 mg of tissue, 300 μl of methanol and the deuterated internal standards were added and tissues were homogenized in a Precellys 24 Dual system equipped with a Criolys cooler (Precellys). Samples were analyzed using an Acquity UPLC system (Waters, UK) equipped with an Acquity UPLC BEH C18 column (1.7μm, 2.1 x 100 mm; Waters). The MS analysis was performed using a Waters Xevo TQ-XS mass spectrometer (Waters) with an ESI source working in the negative-ion mode.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/280249
https://doi.org/10.20350/digitalCSIC/14755
url http://hdl.handle.net/10261/280249
https://doi.org/10.20350/digitalCSIC/14755
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108977RB-I00
Microsoft
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/vnd.ms-excel
dc.publisher.none.fl_str_mv DIGITAL.CSIC
publisher.none.fl_str_mv DIGITAL.CSIC
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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